Posted by admin on Jun 30, 2016 in |
Ayurveda is the primeval complete serving system in medical field. However, one of the barriers in the acceptance of the Ayurvedic formulation is the paucity of standard quality control outline. World health organization (WHO) in 1999 has given a detail procedure for the standardization of herbal drugs comprising of a single content but not for standardization of polyherbal formulations. Mahashankhvati is official in Ayurvedic Formulary of India and is prescribed for treatment of haemorrhoids, malabsorption syndrome, dyspepsia and indigestion. In the proposed work, attempt has been made for standardization of Mahashankh Vati by developing chromatographic method. Piperine from Piper longum and Piper nigrum, Umbelliferone from Ferula asafoetida and Gallic acid from Terminaliachebula present in formulation were selected as marker compounds. A new, rapid, simple, precise, selective HPTLC method was developed for marketed preparation of Mahashankhvati. The separation was performed on TLC aluminium plates precoated with silica gel 60 F254, using toluene: ethyl acetate: methanol: formic acid (7:2:2.5:0.5 v/v/v/v) as mobile phase. The densitometric analysis was carried out at the...
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Posted by admin on Jun 30, 2016 in |
Protein kinases are key players in cellular signal transduction and perturbations in its activity leads to cancer. Targeting these seems to be sufficient to provoke apoptotic response, thus limiting the process of tumorogenesis. Among kinase inhibitors, imatinib has demonstrated magnificent clinical efficacy. But patients with advanced disease harbor faces resistance. Currently there is requisite of making strategies to overcome resistance and adverse effects in order to provide maximum benefit to the patients. Present work was to develop a new analog of Imatinib with the objective to provide a molecule with best combination of properties, safe and affordable drug. For this purpose Imatinib was taken as a lead molecule which has shown a successful positive bioactivity score of 0.59 for kinase receptor predicted using Molinspiration software. Imatinib was optimized using the concept of Bioisosteric replacement, in order to achieving better affinity towards protein kinase receptor. Newly designed analogs were found in compliance with Lipinski rule of five recommendations for new chemical entity to have good oral bioavailability with no violations....
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Posted by admin on Jun 30, 2016 in |
Oral squamous cell carcinoma is one of the leading causes of mortality in India. The various haematological and biochemical changes including liver enzymes imbalance occur in cancer treatment. The careful monitoring of serum biochemical and haematological changes had very important role in prognosis of disease. Therefore, the present study aimed to study haematological and biochemical changes in pre and post treatment of buccal mucosa carcinoma patients. In study, totally 198 buccal mucosa carcinoma subjected were included from both genders. We observed the Haematological parameters (level of completer haemogram, differential leucocytes count), biochemical and liver function parameters in pre and post treatment of subjects. The serum biochemical and haematological levels were lower in post treatment groups than before treatment of subjects, whereas glucose level did not show significant changes. Hence, the subjects did not affected by diabetics. Most of subjects had anaemic, treatment severely affected bone marrow. However, these changes were exists statistically significant by paired samples t-test at p˂0.001. The biochemical and haematological changes were additional risk for subjects....
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Posted by admin on Jun 30, 2016 in |
A simple, precise, accurate, reproducible and economical reverse phase liquid chromatography method was developed and validated for the quantitative simultaneous estimation of Bisoprolol Fumarate and Hydrochlorothiazide in bulk and marketed formulations. Estimation of drugs in this combination was done with a C18 column Kromasil 100-5C18 column [250mm x 4.6mm].using mobile phase of composition Acetonitrile and phosphate buffer (40:60 v/v, pH 3).The flow rate was 1 ml/min and the effluents were monitored at 228 nm. The retention time of Bisoprolol Fumarate and Hydrochlorothiazide were 3.3 min and 6.25 min respectively. The method was found to be linear over a concentration range of 20-100 mg/ml for both Bisoprolol Fumarate and Hydrochlorothiazide. The established method proved as reproducible one with a %RSD value of less than 2 and having the robustness and accuracy within the specified limits. Assay of marketed formulation was determined and find with 98.1% and 97.6% for Bisoprolol Fumarate and Hydrochlorothiazide respectively. The method was validated according to the guidelines of International Conference on Harmonization (ICH) and was successfully...
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Posted by admin on Jun 30, 2016 in |
The objective of the present investigation was to evaluate the antibacterial and antioxidant potential of n-hexane and methanolic extracts of Sterculia foeida L. bark. The antibacterial potential of Sterculia foetida L. bark was tested against human pathogens causing diarrhoea and dysentery such as Shigella flexneri (MTCC-9543), Salmonella enterica ser typhi (MTCC-733), Bacillus subtilis (MTCC-1305), Streptococcus mitis (MTCC-2897), Klebsiella pneumoniae (MTCC-109) and Staphylococcus aureus (MTCC-1430) by using agar well diffusion method. The result of the study revealed that n-hexane extract was highly effective against Shigella flexneri, whereas Klebsiella pneumoniae showed no response and Salmonella enterica ser typhi, Bacillus subtilis, Streptococcus mitis, Staphylococcus aureus responded moderately. The methnolic extract had high inhibition against Salmonella enterica ser typhi (15.16 ± 0.20 mm) whereas Shigella flexneri showed no response and moderate effect against other test bacteria. The antioxidant activity by DPPH scavenging method resulted in significant antioxidant potential of n-hexane and methanolic extracts with IC50 value of 51.26 and 66.84 respectively. Ciprofloxacin was taken as the reference...
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