Posted by admin on Jun 30, 2016 in |
The crude methanolic study of Vanda roxburghii root was investigated for antioxidant, analgesic and cytotoxic activity. The extract of Vanda roxburghii root showed antioxidant activity in DPPH radical scavenging activity, nitric oxide scavenging power assay. In DPPH and NO scavenging assay the extract shown adequate antioxidant activity and the IC50 values in DPPH radical scavenging and NO scavenging assays were found to be 113.35±1.27 and 127.31±0.26ug mL-1 while the IC50 values of ascorbic acid were 12.30±0.11 and 18.64±0.22 ug ml-1. Reducing power activity of the extract increased in a dose dependent manner. Analgesic activity of the crude extract was evaluated using acetic acid induced writhing model of pain in mice. The crude extract at 200 and 400 mg kg-1b.wt. doses displayed significant (p<0.001) reducing in acetic acid induced writhing in mice with a maximum effect of 75.89% reduction at 400 mg kg-1b.wt. Which is comparable to the standard diclofenac sodium (86.52%). The extract was also investigated for cytotoxic activity using Brine Shrimp lethality bioassay. In this the extract showed...
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Posted by admin on Jun 30, 2016 in |
The best QSAR model were generated with left of adept and significant descriptors like electronic, lipophilic and topological, using multiple linear regression (MLR) and partial least square (PLS), model further explained by using forward feed neural network analysis (FFNN). QSAR is a kind of technique that directly correlates in between chemical structure to their biological activity. The best MLR statistical expressions were evaluated with good predictive and authenticated ability and the values were S =0.367, F =53.06 r =0.910, r² =0.828, r2(cv)=0.780. The r2 (training and test-set) values of MLR, PLS and FFNN are 0.82, 0.71, 0.82, 0.71 and 0. 81, 0.74 respectively, which predicts the soundness of the model. The model reveals that total dipole moment, bond lipole and kappa 3 are prerequisite descriptors for determining further promising GSK-3β antagonist with high and liable potency against target. In addition to QSAR modelling, Lipinski’s rule of five was employed on a series and we found no violation in it, which means 3-aryl- 4-(arylhydrazono) 1H pyrazol-5-ones has enough good pharmacokinetic...
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Posted by admin on Jun 30, 2016 in |
Objective: Conventionally, sustained release pellets have been prepared in the form of reservoir systems by coating non-pareil pellets which is an expensive and tedious process. The aim of present study was to develop sustained release matrix (SRM) pellets of Nateglinide (NT), an oral antidiabetic agent, commonly used in the treatment of Type 2 Diabetes mellitus. Methods: The SRM pellet formulations of NT were prepared with different polymers like HPMC K4, HPMC K15, Kollidon® SR (K-SR), Hydrogenated castor oil (K-HCO) by extrusion spheronization process. The SRM pellet formulations were characterized for micromeritics properties, aspect ratio, sphericity, friability, Kawakita analysis, Fourier transform infrared spectroscopy (FTIR), drug content and In vitro drug release studies. Results: The optimized formulation of SRM pellets (Batch F7) prepared by using K-SR (35%w/w) and K-HCO (15 %w/w) has shown desired sphericity and excellent micromeritics properties. The friability and drug content value were found in the range of 0.22- 0.81% and 96.8-99.7% respectively for all formulations. FTIR spectroscopy studies have not revealed any chemical interaction between the NT...
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Posted by admin on Jun 30, 2016 in |
Acne vulgaris, the inflammatory disease of sebaceous follicles of skin with Propioni bacterium acnes (an anaerobic pathogen) play deliberate role in its pathogenesis by inducing certain inflammatory mediators. The aim of present study was to evaluate the antibacterial activity of crude ethanolic extract of Mentha piperata and essential oil of the peel of Citrus limetta (extracted by hydrodistillation process) and control (Tetracycline) against Propioni bacterium acnes (MTCC 1951). Under the technique of Broth Micro dilution method, recommended by CLSI (NCCLS), for MICs as well as IC50 values analyzed. The oil of Citrus limetta (MIC: 2.99, IC50: 1.889) found to be highly active. Mentha piperata shows comparatively low activity (MIC: RANGE, IC50:0.345). The activities were compared with reading of standard...
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Posted by admin on Jun 30, 2016 in |
A simultaneous stability-indicating reversed-phase high performance liquid chromatography (HPLC) method for analysis of abacavir (ABC), dolutegravir (DTG) and lamivudine (3TC) as the bulk drug and in the formulation was developed. Compounds were separated on Kinetex 5 µ C18 100 A (250 mm x 4.6 mm). A gradient program of mobile phase at different proportions of acetonitrile (ACN) and water was used. The retention times of ABC, DTG and 3TC were 5.2, 8.4 and 3.1 minutes (mins) respectively. The drugs were subjected to the stress conditions of acid, base, oxidative, hydrolytic, humidity, thermal and photolytic degradation. The degradation products were well resolved from main peak and its impurities, proving the stability-indicating ability of the method. The method was linear in the concentration range of 20–100 μg/mL, 2-16μg/mL and 10-80 μg/mL for ABC, DTG and 3TC respectively. The method was accurate and precise with a limit of detection and limit of quantitation of 2.05 and 6.73 µg/ mL, 0.28 and 0.94 µg/ mL and 2.32 and 7.72 µg/ mL for ABC,...
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