Posted by admin on Jun 30, 2016 in |
Mentha piperitais currently one of the most economically important aromatic and medicinal crops in all over the world. Phytochemical investigation of the leaves of Mentha piperita (L.) (Lamiaceae) yielded a new compound, which is decarboxyrosemarinic acid galactoside. Formulate as 3’, 4’-dihydroxy-β-phenyl ethyl caffeate-4-(3’’’-menthyl)-4’-β-D-galacto pyranoside. 13C NMR, 1H NMR, FABMS, IR were used for structural characterization. Wound healing responses of this compound was evaluated from biochemical as well as biophysical parameters and analyze the role of isolated compound. The parameters studied included rate of wound contraction and the period of epithelialization in excision wound model. Tissues were removed on different days of intervals and subsequently analyzed for specific assays Tensile strength in incision wound model was assessed along with histopathological examinations. Compound-1was found to increase the cellular proliferation and collagensynthesis at the wound site, as indicated by increases in amounts of DNA synthesized, protein content. Compound-1treatment was also shown to decrease the levels of lipid peroxides (LPs), while the activity of enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase...
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Posted by admin on Jun 30, 2016 in |
Paracetamol tablets are popular over the counter (OTC) products among the patients as a good analgesics and antipyretics. The objective of this study was to compare the quality of the paracetamol tablet formulations those are locally available in Trinidad & Tobago pharmaceutical market manufactured by various pharmaceutical companies with pharmacopoeia standards. The four popular brands (A, B, C, D) of paracetamol conventional tablet of 500 mg strength were chosen. The paracetamol tablets were obtained from government hospital pharmacies as well as from local private pharmacies. To compare the quality of tablet formulations of different brands various official parameters like friability, weight variation, disintegration time, dissolution and drug assay tests were performed as per the pharmacopoeia. The result of all these parameters of different brands were in the pharmacopoeial limits so it could be concluded that marketed pharmaceutical tablets of paracetamol of these brands are safe, effective and efficacious as well as satisfy quality control limits of...
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Posted by admin on May 31, 2016 in |
Inhibition of viral coating protein is an established therapeutic strategy for the treatment of many influenza treatment regimes. Although resistance to such inhibitors is common phenomena in different influenza treatment, such resistance can be averted by targeting coating proteins with phytochemicals. Swine Flu neuraminidase protein (H1N1) has been currently focused target for influenza inhibitor research. We used computational high throughput screening approach to identify novel phytochemical inhibitors from phytochemical knowledgebase. Our computational method used in this study is an integration of qualitative models obtained from consensus hydrated virtual screening protocols for H1N1 Neuraminidase 1 (N1) to identify the novel phytochemical inhibitors. Using the available N1 crystal structures in protein databank flexibility and hydration states were analyzed and validated. The three representative crystal structures with open and closed conformations with highly conserved waters were used to screened the phytochemical database and identified novel inhibitors, among them a first in class alkaloids inhibitor that have shown better affinity against neuraminidase protein over marketed drugs. Our studies suggest that this computational screening...
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Posted by admin on May 31, 2016 in |
Background – Drugs affecting gastrointestinal motility find place in the treatment of gastrointestinal motility disorders. Objective: To evaluate the effect of erythromycin base on the isolated tissue of rabbit jejunum, guinea pig ileum & rat colon in vitro and also to see whether it alters the contractile responses induced by acetylcholine. Materials and Methods: a) Rabbit jejunum experiment: After recording the baseline spontaneous rhythmic contractions, erythromycin was added to the organ bath and the responses were taken every 3 min for 30 min. The effect on frequency & the amplitude of spontaneous contractions were noted. (Erythromycin doses used – 1, 2, 10 & 20 µM; in six rabbits). b) Guinea pig ileum & rat colon experiment: Initially responses were recorded with acetylcholine (1-100µg). After giving wash, erythromycin was added to the organ bath and response was recorded for 30 seconds; then after a contact period of 3 min with erythromycin, responses to selected dose of acetylcholine were recorded again. (Erythromycin doses used – 100, 300 500 & 1000 µM;...
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Posted by admin on May 31, 2016 in |
In diabetes mellitus antioxidant vitamins are commonly employed to prevent or mitigate the long term complications of the disorder. Diabetic retinopathy, nephropathy, coronary and cerebral vascular insufficiency are examples of such complications. They are more common in Type 2 diabetes mellitus. The present study was conducted to observe the effect of Vit A on blood glucose level and its possible interactions with commonly prescribed oral antidiabetic agents i.e. glimepiride, gliclazide and metformin. The study was performed on healthy albino rabbits of either sex in which hyperglycemia was induced by giving oral glucose. We observed that Vit.A significantly lowered the blood glucose level but antagonized the effect of oral antidiabetic agents in co-administration therapy. It is therefore advisable that caution must be exercised when giving high doses of VitA to produce antioxidant effects in diabetic patients because it is not only unnecessary but may also have potentially deleterious effect on glycemic...
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