Posted by admin on May 31, 2016 in |
A series of 2,4-diaminotriazines were synthesized as Mycobacterium tuberculosis(Mtb) dihydrofolatereductase (DHFR) inhibitors. These derivatives were evaluated in whole cells by employing resazurinmicrotitre plate assay (REMA) against MtbH37Rv. Further, these were tested against other gram positive and gram negative bacterial strains to check specificity for Mtb. Cytotoxicity assessment was performed using HepG2 cell line and the compounds were found to be non-cytotoxic. The results indicated that some of the derivatives exhibited promising activity. The most active compound in the REMA assay was selected for DHFR enzyme assay against both the Mtb and human enzymes. The enzyme assay results indicated that this derivative exhibited selectivity towards the pathogenic enzyme. The most active compound in the whole cell assay against MtbH37Rv showed low cytotoxicity, was specific towards Mtb and displayed selectivity in the DHFR enzyme assay. Thus this study provides promising insight for design of potent and selective Mtb DHFR...
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Posted by admin on May 31, 2016 in |
Bacteriological qualities of three different brands of chloroquine syrup (EZR, DMR and MBR) commonly sold in Calabar municipality, Nigeria were evaluated using standard microbiological techniques. Each brand was evaluated in duplicates and examined visually for possible abnormalities such as colour, turbidity and un-usual odour. This was followed by determination of viable cell count and identification of bacterial isolates. Chloroquine syrups sample EZR and MBR had mean bacterial count of 1.0x103cfu/ml respectively and DMR had 3.0x103cfu/ml. The isolates were staphylococcus aureus (47%), Escherichia coli (38%) and Pseudomonas aeruginosa (15%). All strain of S. aureus was sensitive to gentamycin (CN), ciprofloxacin (CPX), septin (SXT), Erythromycin (E) and resistance to Taravid (OFX), Amoxacillin (AM) and Ampicillin (AP). Escherichia coli strains isolated showed sensitivity to ciprofloxacin (CPX), Taravid (OFX) and septin (SXT) and was resistance to gentamycin (CN), Amoxacillin (AM) and Ampicillin (AP). P. aeroginosa strains were sensitive to Ciprofloxacin (CPX) and Taravid (OFX), and was resistant to Gentamycin (CN). The colony forming unit (cfu) for the sample DMR found in the study...
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Posted by admin on May 31, 2016 in |
This study compares the performance of Mycobacterium Growth Indicator Tube (MGIT) culture against conventional Lowenstein Jensen (LJ) media and direct AFB smear examination in pulmonary and extra pulmonary tuberculosis specimens for isolation and drug sensitivity testing (DST) using solid (LJ) and liquid (BACTEC-MGIT-320) media at a tertiary care centre in North India A total of 132 consecutive clinical samples were obtained from suspected cases of TB for smear-microscopy and culture on LJ and BACTEC-MGIT-320. Positive cultures underwent DST for streptomycin, isoniazid, rifampicin and ethambutol. A total of 132 specimens were cultured on LJ and the BACTEC-MGIT-320. The mean (range) time to detection was 14.2 (3–40) and 23.2 (8–42) days for MGIT-320 and LJ, respectively 69 (52.3%) were positive with either MGIT or culture of which 45 (65.2%) were smear positive and 24(34.8%) were smear negative. On comparing MGIT and LJ culture, 65 (94.2%) were positive with MGIT-320 whereas 62(89.8%) LJ cultures were positive DST was conducted on both MGIT-320 and LJ media in the 58 concordant culture-positive isolates. Thirty...
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Posted by admin on May 31, 2016 in |
The electrochemical behavior of Manidipine is studied by employing cyclic voltammetry (CV) and Differential Pulse Polaro graphy (DPP) in universal buffers of PH ranging from 2.0 to 12.0. The kinetic parameters, such as transfer co-efficient, diffusion co-efficient, and heterogeneous forward rate constant values are evaluated by employing these techniques. Differential Pulse Polarography is employed for the estimation of Manidipine in selected pharmaceutical...
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Posted by admin on May 31, 2016 in |
Introduction: Proteolytic enzymes like serratiopeptidase, trypsin and chymotrypsin are used for prolonged period for edema resolution in post-operative patients, arthritis, tooth extraction etc. either alone or with other anti-inflammatory agent. Various trials have supported and refused the role of enzymes as anti-inflammatory agents. No study was found for analgesic property of proteolytic enzymes. Aims: This study was designed to investigate the anti-inflammatory and analgesic activity of these three proteolytic enzymes alone and in combination with diclofenac at therapeutic doses. Methods and Material: Carrageenan induced paw edema was used for acute inflammation. Edema was measured with the help of plethysmometer at 0 hour and 3 hour. Modified formalin test was used to evaluate the analgesic property. 1% formalin was injected into the forepaw and scoring was done by appropriate and standard method. Statistical Analysis: Edema changes of hind paw and licking duration in seconds of a fore paw with different treatments were compared with one-way ANOVA followed by Tukey-Kramer multiple comparison test with the help of graph pad instat software....
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