Posted by admin on May 31, 2016 in |
The present work was designed to perform the comparative study between the seeds of two selected plants i.e., Butea monosperma & Nigella sativa. The study includes phytochemical screening of primary and secondary metabolites, physico-chemical analysis, pharmacological studies, elemental analysis, and flouroscent studies. The comparative priliminary studies of Butea monosperma and Nigella sativa seeds in different solvent extracts shows the presence of primary metabolites such as carbohydrates, proteins, lipids and secondary metabolites such as Alkaloids, phenolic compounds, Flavonoides, Steroids, Tannins & saponins etc. In the preliminary screening of Nigella sativa ethanolic extract was more effective and shows the presence of maximum number of secondary metabolites, whereas In the preliminary screening of Butea monosperma methanolic extract was more effective and shows the presence of maximum number of secondary metabolites. The pharmacognostic studies was also carried out in order to check the adulteration and quality of crude drugs, which involves the evaluation of organoleptic characters such as colour, odour, taste and other macroscopical physical characters. The comparative physicochemical analysis of total ash...
Read More
Posted by admin on May 31, 2016 in |
Patients with DM are often afflicted by debilitating urologic complications. The main aim of this study is to investigate the effect of streptozotocin (STZ)-induced type I diabetes on contractile responses to numerous stimuli, intracellular cation concentrations and morphology in the rat. Adult rats (n=12) were humanely killed and detrusor muscles of urinary bladder located and excised rapidly and placed in organ baths. Then urinary bladder detrusor muscles were blotted, weighed and dissolved in concentrated nitric acid for the measurements of cation contents. The diabetic rats had significantly (P<0.05) elevated blood glucose compared to control rats. The contractile responses induced by EFS were significantly (P<0.05) increased in the diabetic urinary bladder tissue in comparison to the control urinary bladder tissue. The increase in the contractile force was calcium-dependent in both control and diabetic urinary bladder tissues. The results also showed no significant differences in the levels of sodium, potassium, calcium, between the diabetic and control urinary bladder (p>0.05). However, there was a significant (P<0.05) decrease in magnesium in the diabetic...
Read More
Posted by admin on Apr 30, 2016 in |
The purpose of research work was to develop and optimize mucoadhesive microspheres of levocetirizine for nasal delivery with the aim to enhance the residence time and improve therapeutic efficacy and at the same time increase the Local absorption of drug and reducing systemic side effects and also to develop unique delivery system for patients suffering from allergy and rhinitis. Chitosan (mucoadhesive) based microspheres of levocetirizine were prepared by emulsification-crosslinking method. Glutaraldehyde was used as crosslinking agent. The mean particle size was significantly increased when high concentration of chitosan was used. Aqueous to oil phase ratio, stirring rate and dioctyl sodium sulfosuccinate (DOSS) concentration also influenced the particle size distribution of the microspheres. Microspheres were evaluated with respect to the production yield, particle size, entrapment efficiency, swelling index, FT-IR, in vitro mucoadhesion, % cumulative drug release, histological study and stability studies. Formulation Lf3 was found to be optimized. The optimized formulation Lf3 was mucoadhesive in nature which adhere onto the mucus and increase the residence time within the nasal...
Read More
Posted by admin on Apr 30, 2016 in |
A wide range of compounds are found to be effective against cancer for example Vitamin D3, lysine, proline, gallate, quercetin, etc. Glucosinolates from cabbage are secondary metabolites hydrolyzed by the enzyme myrosinase 1. Their breakdown products such as isothiocyanates, nitriles and thiocyanates, are chemically very reactive, and they are known for their bioactive characteristics, such as anticarcinogenic, fungicidal and bactericidal properties 2. The objective of this study was to formulate a nutrient medium containing glucosinolates from cabbage, Vitamin D3, proline and selenium and study its effect on the SGOT, SGPT and ALP levels. To see the synergistic effect of these nutrients, along with glucosinolates from cabbage two nutrient medium were formulated, NM1 (100 mg/kg BW of rats of Brassica oleracea extract+ 25 mg/kg BW of Vitamin D3+ 4 µg/kg BW selenium+ 500 mg/kg BW of proline) and NM2 (200 mg/kg BW of rats of Brassica oleracea extract+ 50 mg/kg BW of Vitamin D3+ 6 µg/kg BW selenium+ 1000 mg/kg BW of proline) 3. These NM were administered to Wistar...
Read More
Posted by admin on Apr 30, 2016 in |
We have carried out solid supported ,solvent free condition, microwave assisted rapid synthesis of 1- (2,5- substituted diphenyl)-1, 3, 4- oxadiazol -3(2H) – yl) ethanone (3a-3k) by cyclization of N’- (substituted phenyl methylidene) substituted benzohydrazide (2a-2k) in the presence of acetic anhydride and silica gel ( solid support) for 3-4 minutes by microwave irradiation giving excellent yield in short reaction time are notable advantages of this method. The structure elucidations of all the synthesized compounds have been accomplished by elemental analysis, IR, NMR and Mass spectroscopic method. The synthesized compounds (3a-3k) were screened in vitro for their antibacterial activities against S. aureus ( gram positive ) and E. coli ( gram negative ) by cup plate method . Some of the products of series were found to have quite good activities as compared to the standard drug streptomycin. The introduction of -F, -NO2, -Cl (electron withdrawing) group enhancing antibacterial...
Read More
