Posted by admin on Mar 1, 2016 in |
Cancer is a global problem. However, in low-income and developing countries, the expenditures associated with its treatment are increasing disproportionately with resources available. Hence, reducing the drug wastage and financial burden placed on patients, family and society in general is becoming imperative. Our aim was to calculate therapeutic and economic benefits derived by the use of a centralized unit, which allows residual amounts of unused drugs to be reused by patients whose treatments are elaborated in the same working day. We calculated in a comprehensive manner the number of spared vials (flasks) for seven drugs generated from residual amounts of the same working day and, its consequent therapeutic and/or economic benefits. We did not take into account prescribed drug dosages that fitted exactly with doses contained in a vial. Over a six month period, there were: a total of 1524. 07 saved vials with an approximate consequent therapeutic benefit of 123.57 patients and, considering the price of the centralized unit, a total economic benefit of approximately 134, 348 (€),...
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Posted by admin on Mar 1, 2016 in |
Over the last decade the incidence of candidiasis has dramatically increased. To fight candidal infections vaccines with immune modulating properties have been actively investigating in recent years. In our previous studies it was found that the immunobiological drug under the conditional name “Candidocyde” based on the antigens of C. albicans and C. tropicalis fungi possessed the protective and therapeutic effect. The aim of this work is to substantiate experimentally the preservative in the composition of the immunobiological drug based on the antigens of C. albicans and C. tropicalis fungi. Various preservatives (phenol, merthiolate and formaldehyde) in the composition of the immunobiological drug have been studied. Compatibility of the preservative and the immunobiological drug was determined by the antibody titers for preventing and treating candidal infections in mice. The results of the ELISA study when preventing candidal infection have shown that after double injection of the immunobiological drug with phenol in the concentration of 0.25 % and merthiolate in the concentration of 0.01 % as preservatives there is 8 times...
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Posted by admin on Mar 1, 2016 in |
The interaction of losartan potassium (LP) with bovine serum albumin (BSA) was studied by fluorescence quenching in combination with UV–Vis spectroscopic method under near physiological conditions. The fluorescence quenching rate constants and binding constants for BSA–LP system were determined at different temperatures. The fluorescence quenching of BSA by LP is due to static quenching and energy transfer. The results of thermodynamic parameters, ∆H (‒134.3 kJ mol‒1), ∆S (‒368 J mol‒1 K‒1) and ∆G (‒24.52 to ‒20.83 kJ mol‒1), indicated that van der Waals interaction and hydrogen bonding played a major role for LP–BSA association. The competitive experiments demonstrated that the primary binding site of LP on BSA was located at site II in sub-domain IIIA of BSA. The distance between LP and a tryptophane unit was estimated to be 3.183 nm based on the Förster resonance energy transfer theory. The binding constant (Ka) of BSA–LP at 298K was 1.932×104 L mol‒1. Synchronous fluorescence and three-dimensional fluorescence studies showed that the presence of LP could change the conformation of BSA...
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Posted by admin on Mar 1, 2016 in |
The aim of the study to determine antioxidant and cytotoxic properties of the bark extracts of Syzygium cymosum along with phytochemical study for the presence of phytochemical constituents. Total phenolic and flavonoid content of the S.Cymosum extracts was determined by using the Folin- Ciocalteu reagent and aluminum chloride (AlCl3) method, respectively. The crude methanolic extract of S.Cymosum was studied to evaluate its antioxidant activity by DPPH radical scavenging assay and reducing power assay. Brine Shrimp Lethality Bioassay was done for cytotoxic activity. Bark extracts of S.Cymosum have been shown to possess phytoconstituents including carbohydrates, alkaloid, glycosides, steroids, tannins and saponin. The extract was found to contain significant amount of phenol and flavonoid in Folin-Ciocalteau and total flavonoid content assay. Total antioxidant capacity of the extract was estimated to be 505.5±3.535 mg/g of ascorbic acid equivalent. Its IC50 value in DPPH method was 64.99079µg/ml. The extract showed concomitant increase in reducing power with the increase of concentration of the extract. In case of brine shrimp cytotoxicity assay, LC50 value was...
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Posted by admin on Mar 1, 2016 in |
Background: Currently oral bisphosphonates such as alendronate are the preferred therapeutic option for the treatment of postmenopausal osteoporosis by increasing bone mineral density and decreasing both vertebral and non-vertebral fracture risk. Silymarin is mainly used as hepato-protectant but in recent studies on ovariectomized rats, it was found that silymarin can be a promising antiosteoporotic pharmacological agent and selective estrogen receptor modulator. Objective: To evaluate the clinical outcome of combination therapy of alendronate and silymarin on postmenopausal osteoporotic women compared to the monotherapy of each. Methods: This was prospective, interventional, randomized study. A total of 69 patients were classified into 3 groups: Group1 (n=22) received 70 mg alendronate tablet once weekly for two years, Group 2 (n=23) received 140 mg silymarin capsule three times daily for two years, Group 3 (n=24) received 35 mg alendronate once weekly and 140 mg silymarin three times daily for two years. The bone mineral density of the patients was monitored using DXA scan at 0,1 and 2 years. Results: Silymarin showed improvement in the...
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