Posted by admin on Jun 30, 2016 in |
Combination drug formulations are better in terms of effectiveness and hence are used many times for treatment of diseases. The physico-chemical properties of the different API used in a formulation have a significant impact in the development of a single method for the analysis of such drugs. In the current research a rapid analytical method employing GC with Mass Spectroscopy detection has been developed and validated for simultaneous quantification of the active ingredients Ornidazole and Miconazole from the cream formulation. The analytes were extracted from cream base and filtered. A 95% dimethyl polysiloxane column with 5% phenyl polysiloxaneis used for the separation of the analytes. The method involves simple temperature gradient and MS detection. Validation of the method showed response was a linear function of concentration in the range 50-150 µg/mL for both Ornidazole and Miconazole. The method was suitably validated and was found to be precise and robust, with recoveries for both the analytes being consistent and complete. The method has been successfully applied for the analysis of samples...
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Posted by admin on Jun 30, 2016 in |
The present study describes the biochemical, antioxidant and anticancer properties of the crude and melanin free ink of Sepia prabahari which is rich in both organic and inorganic components. The quantitative analysis define the presence of protein (1.16 – 1.49 mg/ml), lipid (0.25 – 1.42 mg/ml), carbohydrates (0.01 – 1.14 mg/ml), ash (0.4 & 0.1%) and moisture (93.33%) content. The native PAGE profile showed distinct bands of protein with molecular weight ranging from 3.5 to 205 kDa. The ink is composed of different amino acids, lipids and sugars which was presented by TLC. The ink revealed good antioxidant activity, thereby lowering or retarding the initiation of lipid oxidation process. The ink was screened for its anticancer activity against human breast adenocarcinoma cell line (MCF 7) and it exhibited a strong cytotoxicity by inhibiting the cell growth. The results of comet assay revealed the DNA damage in the cells treated with ink which is a hallmark of apoptosis. Thus the ink, especially the melanin free ink is known to possess...
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Posted by admin on Jun 30, 2016 in |
This research emphasis on microwave assisted extraction of fruits of Sapindus mukorrosai. Aqueous fruit extract is subjected for the isolation of saponins, focusing only on higher molecular weight of saponins using dialysis membrane. On treatment of aqueous solution of silver nitrate with isolated saponin, stable silver nanoparticles were rapidly formed. The green synthesized silver nanoparticles were characterized by UV-vis spectroscopy, scanning electron microscope (SEM), energy dispersive X-ray (EDX). The SEM confirms the synthesis of square shape of nanocrystalline particles with the size range of 60-90 nm. The presence of saponins has confirmed by FTIR spectra. These nanoparticle has been subjected for hypolipidaemic activity in animal model. It showed significant reduction in total cholesterol, triglyceride level, HDL and VLDL...
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Posted by admin on Jun 30, 2016 in |
Antimicrobial peptides (AMP) showed a broad spectrum of cytotoxic activity against cancer cells, which could provide a new class of anticancer drugs. In this study, we have evaluated the cytotoxic and apoptotic activity of magainin II, an AMP compared with 5-Fluorouracil (5-FU) in colo320 DM cancer cells, and magainin II cytotoxicity was assessed on vero cell line by MTT. The cells cultured in the presence or absence of magainin II and 5-FU. The magainin II inhibited cell viability in dose dependent manner was quantified by MTT assay. The IC50 of magainin II was found to be 89.3nM and at this concentration the cytotoxicity was not observed in vero cell line. The cytotoxicity of the magainin II was further confirmed by lactate dehydrogenase (LDH) assay and neutral red uptake assay (NRU). Apoptosis induction was verified by DNA fragmentation analysis. The morphological changes in colo 320 DM cells was measured by DAPI and acridine orange/ethidium bromide staining, showed nuclear condensation, and this substantiates the apoptotic action of magainin II. The cell...
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Posted by admin on Jun 30, 2016 in |
The crude methanolic study of Vanda roxburghii root was investigated for antioxidant, analgesic and cytotoxic activity. The extract of Vanda roxburghii root showed antioxidant activity in DPPH radical scavenging activity, nitric oxide scavenging power assay. In DPPH and NO scavenging assay the extract shown adequate antioxidant activity and the IC50 values in DPPH radical scavenging and NO scavenging assays were found to be 113.35±1.27 and 127.31±0.26ug mL-1 while the IC50 values of ascorbic acid were 12.30±0.11 and 18.64±0.22 ug ml-1. Reducing power activity of the extract increased in a dose dependent manner. Analgesic activity of the crude extract was evaluated using acetic acid induced writhing model of pain in mice. The crude extract at 200 and 400 mg kg-1b.wt. doses displayed significant (p<0.001) reducing in acetic acid induced writhing in mice with a maximum effect of 75.89% reduction at 400 mg kg-1b.wt. Which is comparable to the standard diclofenac sodium (86.52%). The extract was also investigated for cytotoxic activity using Brine Shrimp lethality bioassay. In this the extract showed...
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