Posted by admin on Mar 31, 2016 in |
The present study was carried out to detect the phytoconstituents present in the bulbous part of Eleutherine bulbosa using Gas Chromatography-Mass Spectrometry (GC-MS) analysis. The mass spectrum of the crude ethyl acetate extract was compared with the available library sources (NIST08LIB, WILEY 8 and FAME). A total of 24 compounds were identified. Most of the major constituents were phenolic in nature. A phenolic compound detected to be present in highest concentration (34.20%) was, 1, 8-Naphthalenediol, 2, 7-diacetyl-3, 6-dimethyl. Carboxylic and sterol compounds were present in minute...
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Posted by admin on Mar 31, 2016 in |
Losartan potassium in tablet dosage form, separation achieved with C 18 column (Kromasil 100-5C18250mm x4.6mm E82860 i.d., 5µm particle), ambient temperature with isocratic mode with mobile phase containing 40:60 Acetonitrile: Buffer consist of 10.5mM of disodium hydrogen phosphate and 10mM potassium dihydrogen phosphate, pH 3.5 adjusted by 10 % Orthophosphoric acid and at flow rate 1.0ml/minute the eluent was monitored at 250nm. The selected chromatographic conditions were found to be effectively separate Losartan potassium 9.98min. The proposed method was found to be linear over the range of 6.4-9.6µg/ml. The drug was subjected to oxidation, acid, alkaline, photolysis and heat stress conditions for degradation. The method was validated for specificity, linearity, accuracy, robustness and solution...
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Posted by admin on Mar 31, 2016 in |
L-Glutaminase (EC.3.5.1.2) is an amidohydrolase which catalyses the hydrolytical deamination of L-glutamine resulting in the production of L-glutamic acid and ammonia. L-Glutaminase is gaining marked importance due to its application potential in cancer therapy, food industry and of high value chemicals like theronine. A variety of microorganisms, including bacteria, yeast, and filamentous fungi, from both soil and marine habitat have been reported to produce L-glutaminase .The optimization of L-glutaminase using one of the prominent bacterial isolate (NRBT2822) in batch mode of cultivation showed that a pH of 7.0, temperature of 37°C and agitation rate of 150 rpm are most suitable parameters for maximum enzyme synthesis in presence of 1.0 g/L of L-glutamine as an inducer. The kinetic studies revealed that the Km and Vmax for the enzyme were 66.83 mM and 0.755 mM/min respectively. It was also found that the enzyme showed highest activity at 37oC with a half life of 3...
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Posted by admin on Mar 31, 2016 in |
Diabetic foot ulcer (DFU) is one of the major problems encountered by nearly 15 % of diabetic patients. Combinations of different antibiotics are used to treat the infection which makes the microorganism multi drug resistant. Among various types of drug delivery system, transdermal drug delivery is preferred in treating DFU because it delivers the drug directly to the wound site, which increases the rate of healing. Terminalia chebula (King of medicine) is known to possess wound healing ability and high antimicrobial activity against almost all bacteria isolated from DFU. In this scenario, we have fabricated different combinations of drug loaded composite films of cellulose acetate and chitosan. The formulated films were characterized using UV-visible and FT-IR spectrophotometer. Physicochemical characterization of the films was done with respect to drug uniformity, moisture uptake, folding endurance, drug release and antimicrobial studies. The antimicrobial activity of the films was evaluated for E.coli and B.subtilis and observed a good control of the growth of microorganism. The results suggest that the cellulose based films are...
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Posted by admin on Mar 31, 2016 in |
The present work reports a prospective observational study to determine perioperative usage pattern of analgesics. Both general and regional anaesthesia and also different analgesics which is most effective to manage post operative pain were used. 120 patients were studied randomly as per criteria. Techniques of anaesthesia, pre, peri& post operative vitals and pain scores were noted in selected time interval. Pain score recorded 0-3 as mild, 4-6 as moderate & 7-10 as severe. Of all the analgesics administered in peri & postoperative period, Fentanyl (F) alone & in combination with diclofenac (D) were used maximum in perioperative period. These two groups were compared. In postoperative GA cases, majority patients received paracetamol (P) and tramadol (T) and in regional blocks, Pethidine (PE) Phenergan (PH) combination & tramadol were used. These groups werecompared to evaluate pain perception. Fentanyl alone found to be more effectivein maintaining hemodynamic stability. In fentanyl-diclofenac combination, pulse rate and blood pressure were higher than fentanyl alone. Pain scores were significantly higher in GA compared to regional blocks....
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