Posted by admin on Apr 30, 2016 in |
A simple, rapid and precise stability-indicating ultra high performance liquid chromatography (UHPLC) has been developed and validated for the estimation of telmisartan in drug substance and pharmaceutical dosage form. The chromatographic separation was achieved with a Poroshell 120EC-C18 column (4.6 x 50mm, 2.7µm) by using mobile phase acetonitrile: 50mM ammonium acetate buffer in the ratio of (45: 55 v/v), pH adjusted to 4.5 with acetic acid. The instrumental settings were flow rate of 0.5ml min-1, column temperature at 25 0C and detector wavelength of 290 nm using a photodiode array detector. Telmisartan was exposed to thermolytic, photolytic, acid, base, hydrolytic and oxidative stress conditions and the stressed samples were analyzed by the proposed method. The developed method shows excellent linearity over a range of 100-300 µg ml-1 for telmisartan. The recovery of telmisartan was above 96%. The proposed method was found to be suitable and accurate for quantitative determination and stability study of telmisartan pharmaceutical...
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Posted by admin on Apr 30, 2016 in |
The current investigation was carried out to develop and validate a fairly simple, accurate, precise, reproducible and robust RP-HPLC method for the estimation of Ticagrelor Hydrochloride. The separation was achieved using Agilent Infinity 1220, Infinity Fast-LC (Pressure limit up to 600 bars) with auto sampler and PDA detector. The Chromatographic analysis was performed on ZORBAX Eclipse Plus 300SB C18 (250 x 4.6mm, 5.0 micron, PN 880995-902) column. Mobile phase consist of (A) Acetonitrile: (B) 20mM Potassium dihydrogen ortho phosphate buffer (40:60 v/v) at a flow rate of 1.0 ml/min. The method showed linear in the mentioned concentrations having line equation y = 22.848x + 1.3214 with correlation coefficient R2 of 0.9995. The recovery values for Ticagrelor ranged from 99.63% to 100.34%. The % RSD was 0.49% and 0.54%, respectively for intraday and interday precision. The limit of detection and limit of quantification were 0.05μg/mL and 0.20μg/mL respectively. Newly developed method was statistically validated for accuracy, precision, linearity and solution stability; hence it is directly applicable for the estimation of...
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Posted by admin on Apr 30, 2016 in |
The present work narrates UV-Visible spectrophotometric methods have been developed for the determination of selected drugs viz., Lamotragine (LMT), Venlafaxine hydrochloride(VFX), Valacyclovir hydrochloride(VCL) and Ractopamine hydrochloride(RAC) in bulk and their pharmaceutical dosage forms by using Tetracyanoethylene (TCNE) as reagent. These methods have been developed based on the formation of charge transfer complexes of drugs as n-electron donor with TCNE as π-acceptor. The selected drugs were formed yellow color complex with TCNE in Acetonitrile and exhibited a doublet at 400 & 420nm. Under the optimized experimental conditions, Beer, s law is obeyed over the concentration ranges of 2.5-12.5µg/ml for the all selected drugs. The effect of polarity of solvents, reagent concentrations, LOQ, LOD, and effect of reaction time have been studied and optimized. These methods have been validated in terms of ICH guidelines and applied to the quantification of selected drugs in bulk and dosage...
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Posted by admin on Apr 30, 2016 in |
Postoperative pain management is prerequisite element for care of surgical patient. Objective of this study was to develop and evaluate nefopam hydrochloride loaded poly (meth) acrylates nanospheres (NFH-NS) for post-incisional pain treatment. Nefopam hydrochloride (NFH) is a centrally acting, non-opioid analgesic exclusive for treatment of post-incisional pain. NFH-NS were fabricated using eudragit RL 100: RS 100 by quasi solvent diffusion technique to provide sustained release of NFH. Entrapment efficiency (% EE), drug loading (% DL), z-average, polydispersity index and zeta potential (ζ) of NFH-NS was found 84.972 ± 1.23%, 21.41 ± 2.02 %, 648 ± 4.8 nm, 0.53 and + 4.48 mV, respectively. Scanning electron micrographs asserted smooth morphology and substantial spherical shape of nanospheres. In-vitro dissolution test revealed that NFH-NS followed fickian diffusion for drug transport. Mean dissolution time, dissolution efficiency and difference factor (f1) for NFH-NS was 5.68 h, 68.9 % and 31.83, respectively, which indicated dissimilarity in dissolution profiles as compared to NFH. % EE and % DL of NFH-NS at 25 ± 2°C/60 ± 5...
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Posted by admin on Apr 30, 2016 in |
Glucosamine-6-phosphate synthase (G6PS) (EC 2.6.1.16), is an one of the drug target for the anti microbial species. Systemic anti bacterial and anti fungal infections are of the growing problems in contemporary medicines, however only limited anti bacterial agents are in clinical practice for selective action with low toxicity. Then there is an emergency need for more effective version of existing molecules as well as new potential target specific molecules. In this scenario, our present study is an attempt to find out specific molecules via in silico screening of novel chalcone based series of compounds targeting the glucosamine-6-phosphate synthase. Among the twenty five novel designed chalcones skeleton series of compounds, all of them have found to be successfully docking inside the active binding domain of G6PS target with a binding energy in a range of -7.35 to -9.99 Kcal/mol with predicted IC50 value range of 4.11 micro molar to 47.68 nano molar...
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