Posted by admin on Mar 1, 2016 in |
Male Wistar rats were procured from ‘The Bombay Veterinary College’, Parel, Mumbai. The animals were maintained and housed in cages and were acclimatized in laboratory conditions for ten days prior to the experiment. The experimental rats were fed orally with isoniazid 300 mg/ 70 kg body weight / day; rifampicin 450 mg / 70 kg body weight / day and Aloe vera extract 50 mg/ kg body weight / day for 30 days. Blood samples of the above groups (A to H) were taken after 30th day from cardiac puncture for estimation of mean concentration serum of AST, ALT, ALP, and ACP, bilirubin, total proein, total albumin and total globulin in serum of rats treated with Aloe vera, Isoniazid and Rifampicin independently as well as in combinations. In our study the more commonly measured ‘liver’ enzymes are showing significant increase in the levels of AST, ALT, ALP, and ACP, bilirubin total proein, total albumin and total globulin in serum of rats treated with Isoniazid and Rifampicin individually and in...
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Posted by admin on Mar 1, 2016 in |
Metallic contaminants in the soil are hazardous and their accumulation in plants and water may be dangerous. Microorganisms have properties to adsorb, absorb and accumulate heavy metals. Moreover, they play the role of problem solver by resolving key problems associated with different kinds of metals. The study was undertaken to isolate and identify heavy metal resistant bacteria in soil around iron industries of Sonipat district in Haryana (India). Eight isolates were selected on the basis of heavy metal tolerance and antibiotic resistance in the secondary screening. Out of eight, one of the strain was authentically identified as Rhizobium halophytocola strain KT327204.1 (RT7) on the basis of morphological, biochemical, 16S rDNA gene sequencing and phylogeny analysis. The minimal inhibitory concentration (MIC) of isolate against Mn (7mM), Ni (1mM), Pb (3mM) and Fe (1mM) was determined in solid media. The identified isolate showed optimum growth at 37°C, 7.0pH and at NaCl concentration of 1%...
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Posted by admin on Mar 1, 2016 in |
Several 3, 6-substituted – [1,2,4] Triazolo [3,4-b][1,3,4] Thiadiazole}-2H-Chromen-2-ones(5a-5j) have been synthesized from ethyl-2-oxo-2H-chromene-3-carboxylate (1) through a multistep reaction sequence. Compound 1 reacted with hydrazine hydrate in the presence of ethanol to give 2-oxo-2H-chromene-3-carbohydrazide (2) which on treatment with carbon disulphide and methanolic potassium hydroxide yielded corresponding potassium dithiocarbazates (3). They were then converted in to 3-(4-amino-5-sulfonyl-4H-1, 2, 4-triazol-3-yl)-2H-chromene-2-one (4) by refluxed with aqueous hydrazine hydrate. The title compounds 5a-5j was prepared by condensing 4 with various substituted aromatic acids in the presence of phosphorus oxy chloride. The synthesized compounds have been characterized by the physical (melting point and TLC) and spectral (IR, 1NMR, 13C NMR and MASS) data. All the compounds were screened for their antimicrobial and anti-tubercular activities. The antimicrobial activity was examined against four types of bacteria by cup plate method. Compounds 5b, 5c, 5g, 5e & 5a showed maximum activity at both 50 and 100µg/ml concentrations. Compounds 5a, 5b, 5c, 5d, 5e, 5g, 5h & 5i showed significant activity against ATCC 27294 H37 RV strain and...
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Posted by admin on Mar 1, 2016 in |
A simple high-performance liquid chromatographic method was developed and validated for the determination of Temozolomide in rat plasma using paracetamol as internal standard. The chromatographic separation was carried out with an isocratic elution using mobile phase consisting of 0.5% glacial acetic acid and methanol. Plasma samples were run at a flow-rate of 1 ml/min using a kromasil C18(4.6×250mm) column and ultraviolet detection at a wavelength of 265nm. The retention times of Temozolomide and paracetamol were found to be 6.92 and 11.2 minutes respectively. The linearity of the method was achieved over the concentration range of 10-60µg/ml demonstrated good linearity with correlation coefficient 0.999. The method was validated for accuracy, precision, linearity, recovery in compliance to international regulatory...
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Posted by admin on Mar 1, 2016 in |
Ropivacaine is a promising drug for subarachnoid block because of its lower cardiotoxic and neurotoxic potential, but onset and duration of anaesthesia is also low and motor block is often insufficient which may lead to use of its higher doses. The aim of this randomized double-blinded study was to see whether the addition of small dose clonidine to small dose ropivacaine for spinal anesthesia prolonged the duration of postoperative analgesia while minimizing the side effects associated with higher doses of ropivacaine. We randomized 60 patients to 2 groups receiving intrathecal isobaric ropivacaine 18 mg (2.4 ml) combined with normal saline (Group R) or clonidine 30 μg (Group RC); all solutions were diluted with saline to 2.6 ml. We compared block characterstics, hemodynamic changes, post-operative analgesia and adverse effects of both the groups. Results showed that clonidine not only significantly reduced the onset time both of sensory and motor block, but also prolonged the duration. Hypotension and bradycardia was more with clonidine group during first hour. The addition of clonidine...
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