Posted by admin on Jan 31, 2016 in |
The periodontal tissues undergo constant changes of remodelling when subject to bacterial infection and the resultant host response. The balance between enzymes secreted by the host such as matrix metalloproteinases (MMPs) and their antagonists tissue inhibitor of metalloproteinases (TIMPs) are major contributors to the progression of periodontal destruction. A sub-type of MMPs referred to gelatinases (MMP-2 and 9) are known to be responsible for the pathologic tissue degradation, with MMP-9 playing a potential role in periodontal pocket formation in periodontitis. The use of anti-collagenase host modulatory agents such as doxycycline hydrochloride regulates the extent of resultant tissue destruction and provides an ideal therapeutic approach in the treatment of periodontitis. However, data regarding the effect doxycycline hydrochloride on gelatinases has not been extensively documented. Therefore, the aim of this study was to identify the expressions of MMP-2 and 9 by an immunohistochemical assay in experimentally induced-periodontitis animal models and to evaluate the effects of a prepared doxycycline hydrochloride-impregnated collagen scaffold on their...
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Posted by admin on Jan 31, 2016 in |
The present study was carried out to explore anti-diabetic, antidyslipoproteinemic and anti oxidant activities of Tinospora cordifolia (Menispermaceae) in type 2 diabetic patients. In this study clinical trial was conducted on type 2 diabetic patients attending the diabetes OPD, at Kaya Chikitsha Vibhag, Rajkiya Ayurvedic Chikitsha Mahavidyalaya, Touriya Ganj, Lucknow, following all ethical guide lines. All biochemical assays were carried out by the standard kit methods. A marked increase in plasma levels of fasting blood sugar, lipid profile accompanied with increase in the lipids and apo-protein levels of serum β lipoproteins following decrease in lipid and protein constituents of α lipoprotein were noted in type 2 diabetic patients compared to healthy controls. The results after prescription of Tinospora cordifoia (powered stem; 50 mg/kg body weight, p. o.) for 15 days in type 2 diabetic patients resulted significant decrease in the level of fasting blood sugar, total cholesterol, β lipoproteins and triglycerides. The decrease in lipids and apoprotein levels of β lipoproteins was accompanied with stimulation of plasma lecithin cholesterol...
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Posted by admin on Jan 31, 2016 in |
The aim of the present study is to probe the antimicrobial activities of methanol leaf extract of Leucas sp. Antifungal susceptibilities of certain clinically isolated dermatophytes to methanol extracts of Leucas aspera and Leucas zeylanica leaves were performed using agar well diffusion method. The result obtained shows that all the extracts expressed remarkable antifungal activity with zone of inhibition ranging from 5 to 10mm. Maximum inhibition zone was recorded with Penicillium sp. (10 mm) while minimum inhibition zone of was recorded for Candida tropicalis (3 mm). From the study it is evident that Leucas aspera and Leucas zeylanica possess potential antidermatophytic activity and further study on these plants may lead to explore novel bioactive...
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Posted by admin on Jan 31, 2016 in |
Aim: The present study aimed to evaluate the antioxidant potential and lipid profile parameters, in relation to lipid peroxidation in patients with maternally inherited type 2 diabetes mellitus and in healthy normal individuals. Methods: All standard methods were used to estimate enzymatic antioxidants [Catalase, Copper containing Superoxide Dismutase (CuSOD), Manganese containing Superoxide Dismutase (MnSOD) and Glutathione Peroxidase (GPX)], non enzymatic antioxidant [Reduced Glutathione (GSH)], lipid peroxidation [Malondialdehyde (MDA)]; and lipid profile parameters [High Density Lipoprotein (HDL), Low Density Lipoprotein (LDL), Triglyceride (TG) and Cholesterol]. Correlations between MDA and all parameters were sought using Spearman’s rank correlation coefficient. Results: Significantly lower mean activities of catalase, CuSOD, MnSOD and GSH and significantly higher mean activity of GPX and higher mean concentration of MDA were reported in plasma samples from patients with type 2 diabetes mellitus as compared to healthy individuals. A significantly lower mean level of HDL and significantly higher mean levels of LDL, TG and Cholesterol were noted in patients with type 2 diabetes mellitus than healthy control individuals. Further,...
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Posted by admin on Jan 31, 2016 in |
An accurate, precise and rapid RP-HPLC method was developed and subsequently validated for the determination of Empagliflozin in API. Better separation of the drug was achieved on Intersil column(150x40mm, 5 μm) with the mobile phase consisted of mixture of 0.01 M acetate buffer, methanol in ratio of (30:70v/v ) at flow rate of 2ml/min, with detection at 260nm using PDA detector. The retention time was found to be 1.223min. The method was found to be linear in the range of 2-150ug/ml with a correlation coefficient (r2) of 0.999. The LOD and LOQ of the method were calculated to be 0.7 and 1.91μg/ml respectively. The Precision was estimated by employing repeatability; intra-day and inter-day studies and the results were calculated as %RSD values and were found to be within the limits. Recovery of Empagliflozin was found to be in the range of 99.7-100-% which confirms the accuracy of the method. The proposed HPLC method is validated using standard ICH...
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