Posted by admin on Mar 1, 2016 in |
To provide a scientific basis to traditional uses of seeds of Anethum graveolens, seed extract was evaluated for its antioxidative potential. Firstly, indeed, aqueous extract of Anethum graveolens was prepared and analyzed for the phytochemical compounds contents. Furthermore, antioxidant activity was assessed using the Total Antioxidant Capacity (TAC) methods: 2.2’Azinobis (3-ethylbenzthiazoline-6-sulfonic acid (ABTS. +), 2, 2-Diphenyl-1-picrylhydrazyl (DPPH.+), Ferric reducing antioxidant power (FRAP) and also the Hydroxyl Radical Scavenging Activity (HRSA) assays. The total phenolic, catechins and tannins contents of this extract were also assessed. Finally, the protection effect on DNA damage was evaluated. Results obtained by all of the radical scavenging activity methods used showed higher antioxidant activity. Also, the results indicate that the extracts of A. graveolens showed stronger hydroxyl radical scavenging activity with an inhibition percentage of 43.56 %. However, results obtained showed that the extract was not providing a protective effect upon DNA fragmentation by the method...
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Posted by admin on Mar 1, 2016 in |
A gradient reverse phase Ultra Performance Liquid Chromatographic (RP-UPLC) method was developed and validated for determination of Micafungin sodium and its synthetic impurities. The successful separation of Micafungin sodium and its synthetic impurities was achieved using Phenomenax Aeris peptide XB C18 (150×2.1 mm i.d., 1.7 µ particle size) column maintained at 45 °C temperature with mobile phase consisting 0.01 M phosphate buffer pH 2.9 and acetonitrile in a gradient programme. The mobile phase flow rate was 0.3 ml/min and the detection wavelength was 279 nm. The retention time in developed method is comparatively less than the reported HPLC methods for determination of Micafungin sodium offering less time consuming and fast analytical method. The developed RP-UPLC method was validated according to ICH guidelines with respect to linearity, accuracy, precision, specificity and robustness and also the LOD and LOQ values were...
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Posted by admin on Mar 1, 2016 in |
Women on different contraceptive methods have been linked with the development of various changes in lipids profile. Therefore, the relationship between contraceptive use and lipids changes needs to be investigated. Method: This was a cross-sectional randomized study conducted on non-lactating, non-pregnant women who were on different methods of contraceptives for at least a period of 6 months and were attending the Family Planning room at Al-shomaly hospital south of Babylon government. The study population consisted of a countryside population. One hundred twenty-five divided into five groups 25 women each group: (1): taken oral contraceptive (microgynon tablet), (2) taken oral contraceptive (microgynon tablet) with 400 IU Vitamin E per day, (3) on injectable contraceptive (depo Provera), and (4): on injectable (depo Provera) with 400 IU Vitamin E per day and (5): not taken any things as control individuals. Conclusion: contraceptives have a series role in elevation of lipids profiles, and use of Vitamin E can reduce a degree of these...
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Posted by admin on Mar 1, 2016 in |
Human eye is a challenging subject for topical administration of the drugs because of its peculiar anatomical arrangements of surface tissue and impermeability of the cornea. Topical instillation of drugs through eye drops is the most important and well-accepted route of administration for the treatment of various eye disorders. Conventional ophthalmic drug delivery systems often result in poor bioavailability and therefore poor therapeutic response. Several new preparations have been developed to prolong the contact time of the medicament on the ocular surface. Successful results have been obtained with inserts and collagen shields. However, these preparations have some disadvantages, such as poor patient compliance, especially by geriatric patients. This problem can be overcome by using in situ gel forming systems of polymers that exhibit reversible phase transition. Such system can be formulated as eye drops suitable for administration by instillation into the eye, which upon exposure to the eye converts to the gel phase. The advantage of these formulations is that unlike inserts and films they do not require sophisticated...
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Posted by admin on Mar 1, 2016 in |
A freshwater microalga, Nostoc sp. isolated from Tamdil Wetland of Mizoram was tested for its algicidal, antibacterial and antifungal activity. Extracts were prepared with methanol, ethanol:water and dichloromethane:isopropanol and tested for their antimicrobial activity against nine microorganisms comprising of three chlorophycean algae (Chlorella vulgaris, Scenedesmus quadricauda and Selenastrum capricornatum), one cyanobacterium (Anabaena variabilis), three bacterial strains (Bacillus subtilis, Bacillus pumilus and Escherichia coli) and two fungal strains (Fusarium udum and Fusarium culmorum). Among the three extracts tested the methanol extract was found most effective on algal test organisms. The highest (60%) inhibitory effect was observed against A. variabilis by methanol extract followed by 31% inhibition by dichloromethane:isopropanol. When tested on bacterial strains, the highest inhibition zone (23.67±1.58mm) was observed in B. subtilis by dichloromethane:isopropanol extract followed by ethanol:water extract. In case of fungal activity, the highest inhibition zone (11.00±0.58mm) was observed in F. culmorum by dichloromethane:isopropanol extract and the rest of the extracts showed almost similar effect. MICs of each extract on all the organisms varied. The extracts were...
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