Posted by admin on Apr 30, 2016 in |
Introduction: Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used drugs to manage a variety of acute and chronic conditions associated with pain and/or inflammation. In the Indian market, various NSAIDs group of drugs are available in different formulations. This creates a lot of problem with physician to decide the drug of choice for individual patients. Objective: To evaluate price variation of different single NSAIDs formulations available in Indian market. Materials and Methods: Cost of a particular drug in the same strength and dosage forms being manufactured by different companies was obtained from “Current Index of Medical Specialties” (CIMS) Jan-Apr, 2015 and “Indian Drug Review” (IDR) Issue 1,2015. Difference between the maximum and minimum cost of the same drug manufactured by different pharmaceutical companies was calculated and percentage cost variation was calculated. Spearman correlation analysis and regression coefficient was done to observe the correlation between no. of manufacturing companies and their percentage price variation with the help of IBM Statistical Package for Social Sciences (SPSS v.21). Results: For Oral preparations, Diclofenc(50mg...
Read More
Posted by admin on Apr 30, 2016 in |
Protein glycation is a spontaneous post translational modification of proteins by excess sugars causing formation of advanced glycation end products (AGEs) in diabetic individuals and responsible for diabetes complications. A wide variety of anti-glycating agents have been reported & recently there has been interest in natural products with anti-glycation properties. Momordica charantia L (M. Charantia L) has been used historically for medicinal purposes particularly for treatment of diabetes & cancers. M. Charantia L Extract contains potent antioxidant activity and it can be a useful anti-glycating agent. Material & methods: Human serum albumin was used for in vitro glycation. Various concentrations of extract of M. Charantia L were analyzed. Results: Co-incubation of the M. Charantia L. extract with HSA-fructose mixture intensify the fructose mediated glycation of HSA as indicated by increases fluorescence intensity in tryptophan fluorescence &AGE related fluorescence studies. Conclusion: M. Charantia L. seems to aggravate sugar mediated glycation of the protein and need further studies to pinpoint specific bioactive compounds responsible for the observed...
Read More
Posted by admin on Apr 30, 2016 in |
Objective: To analyze phytochemicals both qualitatively and quantitatively and to estimate in vitro antioxidant activity, total phenolic content and concentration of flavonoids of methanolic extract of leaf, stem and fruit of an important medicinal plant Murraya koenigii Linn. Methods: Phytochemical screening of extracts was carried out. Aluminium chloride colorimetric method was used to estimate TFC (total flavonoid content) and TPC (total phenolic content) was measured by Folin-ciocalteu method. Two different assays- DPPH free radical scavenging and LPO were used for determination of antioxidant activity. Results: Quantitative estimation of primary metabolites showed maximum amount of sugar, starch in stem, while lipid and protein in leaf. The TPC were ranging between 18.4±0.21 to 25.2±0.16 mg GAE/gm DW while TFC were found maximum 13.53±0.16 mg QE/gm DW in leaves. The DPPH radical scavenging capacity was found maximum in leaves (IC50 49.86±1.16) and maximum activity in LPO assay was shown by leaf extract (23.715±1.68 μM MDAg-1DW). Conclusions: These results are suggestive of primary bioactive compounds of commercially importance and also Murraya koenigii Linn...
Read More
Posted by admin on Apr 30, 2016 in |
Senna alata is an important medicinal and ornamental flowering tree, in the subfamily of Caesalpinioideae, which was found in diverse habitat in the tropics. Leaves or sap are used to treat several infections such as skin diseases, bronchitis, asthma and ringworm. The present study was design to investigate for conserve the important medicinal plant by establishing an efficient in vitro seed germination method. The seed germination was gradually decreased by increasing the age of the seeds. The seeds were pretreatment with different chemicals such as, sulphuric acid, hydrochloric acid and distilled water. After pretreatment the seeds were surface sterilized with various concentration of mercuric chloride. The objective of the present study the effect of different pretreatment as in vitro seed germination and seedling development of S. alata Linn. Seed propagation is still used as a specialized tool for breeding and propagation of pathogen-free...
Read More
Posted by admin on Apr 30, 2016 in |
A simple and accurate Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method has been developed for the estimation of ketoprofen in vitro samples and in vivo samples from bulk drug and from administered transdermal dosage form, using Spherosorb S5 ODS of 10cm X 4.6 mm column, (5μm particle size). Mobile phase for in vitro sample analysis and in vivo plasma samples consists of 0.01 M sodium dihydrogen phosphate (pH adjusted to 6.5 with ortho phosphoric acid), methanol, and acetonitrile, 4:3:3 (v/v) respectively. Isocratic elution technique was followed. The flow rate was 0.5ml/min and the detection was monitored out by UV detector at 265nm. The retention time for ketoprofen was found to be 2.982 in in vitro sample and 3.025 min in in vivo sample. Naproxen was used as internal standard for in vivo sample analysis. The proposed method has permitted the quantification of ketoprofen over linearity in the range of 100-1000...
Read More
