Posted by admin on Jan 1, 2016 in |
The use of herbal medicines for the betterment of mankind is well practiced from centuries. Triphala is a well known Rasayana drug used in Indian systems of medicine. Due to its wide spread usage in herbal medicine it drives the attention of regulatory bodies for its safety and efficacy and hence quality become major concern. In the present study we have standardized herbs by Pharmacognostical evaluation and selected chemical markers as part of quality control tool. Gallic acid and total tannins are selected as stability indicating assay for triphala caplet. The drug was subjected to short time accelerated stability study (400C/75%RH) as per ICH guidelines. Presence of stone cells, starch grains and calcium oxalate crystals are noted for differentiate along with the xylem elements (fibers and vessels) based on their structure. The content of these active constituents were monitored throughout the study and were found to be stable (Gallic acid: 3.72 to 5.24 %w/w, Total tannins: (17.16%w/w to 23.49%w/w). The HPTLC chromatographic fingerprint was also found to be complying...
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Posted by admin on Jan 1, 2016 in |
New tetradentate Schiff base and its Co(II), Ni(II), Cu(II), Zn(II), Cd(II) and Hg(II) complexes formed by the condensation of 5-fluoro-3-hydrazonoindolin-2-one with isophthalaldehyde. The coloured complexes were prepared of [MLX2], Where L = Schiff base hydrazone, M = Co(II), Ni(II), Cu(II), Zn(II), Cd(II) and Hg(II), X = Cl-. Physico-chemical characterization has been carried out to determine the structure of the complexes. All the synthesized compounds, were studied for their in vitro antibacterial, and antifungal activities, against two Gram-negative (Shigella flexneri and Enterococcus aerogens) and one Gram-positive (Micrococcus luteus,) bacterial strains and against three fungal strains (Candida krusiae Candida parasilopsis and Malassesia pachydermatis) by using cup-plate method. The DNA cleavage capacity of all the complexes was analysed by agarose gel electrophoresis...
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Posted by admin on Dec 31, 2015 in |
Docking is a method which predicts the preferred orientation of one molecule to a second when bound to each other to form a stable complex and is frequently used to predict the binding orientation of small molecule drug candidates to their protein targets and plays an important role in the rational design of drugs. The present study has been focused on the Docking studies of 1,3-disubstituted ureas derivatives as anti-tubercular agents that can explore the binding affinity of ligands to that of the epoxide hydrolase with the help of Schrodinger molecular modelling software. The G-score of the ligand 6s was found to be -8.03 as comparable with the G-score of co-crystallized ligand i.e. -3.77. The carbonyl oxygen of urea moeity of the ligand 6s showed a H-bond interaction with the phenolic oxygen of TYR381 and TYR465 amino acid of the protein residue with distances 2.24Å and 1.67Å respectively and the carbonyl oxygen of urea moeity of the co-crystallized ligand also showed a H-bond interaction with the phenolic oxygen of...
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Posted by admin on Dec 31, 2015 in |
Studies reported from Ethiopia depict poor health outcomes and management of diabetes cases and low patients’ adherence to recommended regimens including medications. Among factors determining adherence to medications that are amenable to interventions are patient perceptions to their recommended medications. This study thus aimed to assess adherence to anti-diabetic medications and determinant factors including medication related beliefs among type 2 diabetic patients attending their treatment in primary healthcare centers of Addis Ababa, Ethiopia using standardized measures. Methodology: A facility-based cross-sectional study design was followed to assess study participants recruited from among type 2 diabetic patients attending their treatment in three health centers in Kirkos Sub City, Addis Ababa from April to June, 2013. For the assessment interviewer administered structured questionnaires based on standardized measures such as the 8-item Morisk’s Medication Adherence Scale (MMAS) and the specific Belief about Medications Questionnaire (BMQ) were used. Result: A total of 155 study participants, all of whom were on oral hypoglycemic agents were involved in the study among whom 53.5% were male and...
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Posted by admin on Dec 31, 2015 in |
Aim: The present study evaluated the anti-diabetic, anti-hyperlipidimeic, antioxidant activities and renal protective effect of aqueous extract from Ganoderma. lucidum fruiting bodies in streptozotocin induced diabetic rats. Material and Results: Qualitative and quantitative phytochemical analysis for G. Lucidum aqueous extract showed the Presence of high concentration of tannins content. Thin layer chromatography (TLC) revealed the presence one tannin spot which high performance liquid chromatography (HPLC) analysis confirmed that the tannin compound is tannic acid. Extracted tannic acid showed highly antioxidant activity in vitro. Two doses of the extract were administered intraperitoneally. Tannic acid significantly decrease blood glucose levels and significant increase in serum insulin in addition, it improved serum lipid profile in diabetic rats compared to untreated diabetic group. Results: Showed that tannic acid also had potent antioxidant activity in vivo, as it significantly decreased MDA while GSH and GPx in erythrocytic and renal tissues were significantly increased. Histopathological examination of kidney tissue tannic acid treated rats showed mild glomerular atrophy and minimal tubular affection with noticeably dilated blood...
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