Posted by admin on Nov 30, 2015 in |
High performance thin layer chromatographic method has been developed for the determination of Olopatadine (OLO) and Montelukast (MONT) in their combined dosage form. Merck HPTLC aluminum plates of silica gel G60 F254, (10 × 10 cm) was used for separation of combined with 250 μm thickness using Chloroform: Ethyl acetate: Methanol: Triethylamine (6: 4.5: 2.5: 0.8, v/v/v/v) as mobile phase. HPTLC separation of the both drugs were carried out and followed by densitometric measurement was performed in the absorbance mode at 254 nm. The drugs were resolved satisfactorily with Rf values of 0.39 ± 0.01 and 0.66 ± 0.01 for OLO and MONT, respectively. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantification and robustness. The developed HPTLC method can be applied for identification and quantitative determination of OLO and MONT in bulk drug and drug...
Read More
Posted by admin on Nov 30, 2015 in |
A simple, precise, and robust spectrophotometric method for the determination of Cefotaxime sodium has been investigated and validated. The proposed chemical reaction involves the reaction of primary amine of Cefotaxime sodium with nitrite solution in acidic medium to form a diazotized product, followed by the reaction of the diazotized product with suitable coupling reagent resulting in a colored product. Cefotaxime sodium has shown λmax 500nm. The method was found linear obeying Beer Lambert law at concentration range of 1-20 ppm. In the method detection limits was observed 0.744 ppm with quantification limit of 2.48 ppm and standard deviation of 0.248. In the present work an attempt was made to develop a simple, less expensive and more reliable method for the determination of Cefotaxime sodium in pure and pharmaceutical...
Read More
Posted by admin on Nov 30, 2015 in |
Natural products derived from plants and herbs have always had an important role in drug development. Especially in case of cancer, there is a huge demand for effective chemopreventive and chemotherapeutic agents with natural base and low toxic effects on normal non-cancerous cells. In this work, we have studied the effects of Pistacia atlantica sub kurdica (Baneh) extract on microtubule proteins and their biochemical features. Since microtubules are directly involved in spindle formation and cell division, they can be a very good target for anticancer drugs. We hypothesized that Baneh extract has the potential to inhibit microtubule polymerization and dynamics and as a result inhibit spindle formation during cell division. In order to analyze the effect of Baneh extract on microtubule proteins we decided to investigate these proteins biochemical characteristics in the presence of different concentrations of the extract. We used cell culture model to confirm cytotoxicity of Baneh extract. We then did some polymerization kinetics and dynamics study on microtubule proteins in the presence of various concentrations of...
Read More
Posted by admin on Nov 30, 2015 in |
Inflammation is one of the unique mechanism that help body to protect itself against burn, infection, allergens, toxic chemicals, or other noxious stimuli. It is a defense reaction of the body in order to eliminate or reduce the spread of injurious agent. However, over reaction of the body may be harmful or undesirable. This has resulted in extensive development of anti-inflammatory drugs. Despite the benefits that the drugs hold, it also carries the side effects such as gastric lesions, allergy reactions, tolerance and dependence, as well as resistance. Hence, researchers are working towards ideal medicines of anti-inflammatory with highest efficacy, best potency and lowest or none side effects. This research study evaluates the anti-inflammatory activity of trans-cinnamaldehyde and piperine combination. Preclinical testing of cinnamaldehyde and piperine was done earlier individually on inflammatory models but the combination was not validated for the treatment of inflammation. Therefore, the present study was carried out to evaluate the anti-inflammatory potential of the trans-cinnamaldehyde and piperine combination using carrageenan-induced paw edema model. The combination...
Read More
Posted by admin on Nov 30, 2015 in |
Candesartan cilexetil, a prodrug is a racemic mixture containing one chiral center at the cyclohexyloxy-carbonyloxy- ethyl ester group. It is soluble in dimethyl formamide, acetone, methanol, 0.1 N sodium hydroxide solution and insoluble in water. Objective of the present study is to develop a simple, sensitive, accurate, precise and rapid derivative spectrophotometric method for the estimation of candesartan in pure form. For the estimation of candesartan, solvent system employed was absolute methanol and wavelength of detection (λdet) was 294.3 nm for third order derivative spectroscopy. The linearity was obtained in the range 180 – 280 µg/ml. The regression equation was found to be y = 0.0001x + 0.003 & regression coefficient, R2 was found to be 0.999. The limit of detection is 6.7µg/ml and limit of quantification was fund to be 20.4 µg/ml. Obtained results showed that there is minimum intraday and inter day variation. The developed method was validated and recovery studies were also carried out. Sample recovery using the above method was in good agreement with their...
Read More
