Posted by admin on Mar 1, 2016 in |
The aim of the study to determine antioxidant and cytotoxic properties of the bark extracts of Syzygium cymosum along with phytochemical study for the presence of phytochemical constituents. Total phenolic and flavonoid content of the S.Cymosum extracts was determined by using the Folin- Ciocalteu reagent and aluminum chloride (AlCl3) method, respectively. The crude methanolic extract of S.Cymosum was studied to evaluate its antioxidant activity by DPPH radical scavenging assay and reducing power assay. Brine Shrimp Lethality Bioassay was done for cytotoxic activity. Bark extracts of S.Cymosum have been shown to possess phytoconstituents including carbohydrates, alkaloid, glycosides, steroids, tannins and saponin. The extract was found to contain significant amount of phenol and flavonoid in Folin-Ciocalteau and total flavonoid content assay. Total antioxidant capacity of the extract was estimated to be 505.5±3.535 mg/g of ascorbic acid equivalent. Its IC50 value in DPPH method was 64.99079µg/ml. The extract showed concomitant increase in reducing power with the increase of concentration of the extract. In case of brine shrimp cytotoxicity assay, LC50 value was...
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Posted by admin on Mar 1, 2016 in |
Background: Currently oral bisphosphonates such as alendronate are the preferred therapeutic option for the treatment of postmenopausal osteoporosis by increasing bone mineral density and decreasing both vertebral and non-vertebral fracture risk. Silymarin is mainly used as hepato-protectant but in recent studies on ovariectomized rats, it was found that silymarin can be a promising antiosteoporotic pharmacological agent and selective estrogen receptor modulator. Objective: To evaluate the clinical outcome of combination therapy of alendronate and silymarin on postmenopausal osteoporotic women compared to the monotherapy of each. Methods: This was prospective, interventional, randomized study. A total of 69 patients were classified into 3 groups: Group1 (n=22) received 70 mg alendronate tablet once weekly for two years, Group 2 (n=23) received 140 mg silymarin capsule three times daily for two years, Group 3 (n=24) received 35 mg alendronate once weekly and 140 mg silymarin three times daily for two years. The bone mineral density of the patients was monitored using DXA scan at 0,1 and 2 years. Results: Silymarin showed improvement in the...
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Posted by admin on Mar 1, 2016 in |
Vesicovaginal fistula (VVF) is an abnormal fistulous tract that extends between the bladder and the vagina – thus allowing the continuous involuntary discharge of urine into the vaginal vault of the affected patient. This study evaluated presumptively the prevalence and antibiogram of pathogenic bacteria isolated from pre- and post-surgery VVF patients. Urine samples were collected in duplicates from the VVF patients; and these were bacteriologically analyzed using cysteine lactose electrolyte deficient (CLED) agar, MacConkey agar and blood agar. Susceptibility studies were carried out on the isolated pathogens using the disk diffusion method. Klebsiella pneumoniae, Escherichia coli, S. pyogenes and S. aureus were the most isolated bacteria from the urine samples of pre- and post-surgery VVF patients. The organisms showed highest levels of resistance to ceftazidime, cotrimoxazole, amoxicillin, nitrofurantoin, cefuroxime, amoxicillin-clavulanic acid and tetracycline. With the exception of some S. aureus isolates, all the organisms including K. pneumoniae, E. coli and S. aureus were completely resistant to imipenem. However, gentamicin, ciprofloxacin, and ofloxacin showed appreciable levels of antimicrobial efficacy against...
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Posted by admin on Mar 1, 2016 in |
Brhanmanjisthadi Kvatha Curna (BRH) an Ayurvedic preparation, is used as a traditional medicine in filariasis in the northern part of the country. After 45 consecutive days of chronic administration of the BRH preparation in rats its toxicological aspects was studied. In the study the total protein content in the plasma was increased (2.80 %) in BRH treated male rats in comparison to their control male groups. The result showed no significant difference between the control and the BRH treated groups, yet the increase was noticeable (p=0.072). But the albumin content was significantly (p<0.001) increased (17.11 %) in BRH treated male rats & the increase was statistically very highly significant. In the female rats group the total protein (6.80 %) and the albumin content in the plasma were also increased in comparison to their control groups. A statistically very highly significant (p<0.001) increase was noted (15.09 %) only in the case of albumin. In this investigation, none of the changes in terms of the Lipid profile, namely Triglycerides (2.18 %...
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Posted by admin on Mar 1, 2016 in |
The objective of the present investigation was to study the complexation of silymarin, a poorly water soluble herbal drug, with β-cyclodextrin alone and in the presence of sodium carboxymethyl cellulose to evaluate the feasibility of enhancing the solubility and dissolution rate of silymarine. Phase solubility study and kneading method was employed to develop and formulate solid inclusion complexes. Solid inclusion complexes of silymarin –β-cyclodextrin in 1:1 molar ratio were prepared with and without carboxymethyl cellulose by kneading method. Obtained binary and ternary complexes were evaluated for solubility dissolution and physical nature by FTIR, XRD and NMR. The aqueous solubility of silymarin was linearly increased as a function of the concentration of β-cyclodextrin alone and in the presence of sodium carboxymethyl cellulose. Beta cyclodextrin formed binary molecular inclusion complexes with silymarin in1:1 molar ratio as revealed by phase solubility diagram. Sodium carboxymethyl cellulose in ternary complexes has resulted in 2.37 and 1.66 fold enhancements in complexation and solubilizing efficiencies of β-cyclodextrin respectively. Dissolution rate of silymarin in binary and ternary...
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