Posted by admin on Jan 1, 2016 in |
Simple and rapid Reverse Phase High-Performance Liquid Chromatography (RP-HPLC) and UV-spectrophotometry method was developed for the determination of Paracetamol, Pamabrom and Dicyclomine hydrochloride, detection was carried out using photo diode array detector. Chromatographic separation of the analytes was achieved within 3.12, 4.25, 5.35 min for Paracetamol, Pamabrom and Dicyclomine hydrochloride by LC-GC Qualisil Gold-C18 (250 x 4.6 mm i.d., 5µm) column, mobile phase was methanol : water (1% TFA with pH adjusted to 3.0 with ammonia) in the ratio of (83:17), 0.4% (v/v) TEA and was filtered and degassed., flow rate was 1.0 mL/min, and the detection was carried out at 221 nm. Calibration curve was linear (r2=0.9993) in the range of 13-78 g/mL for Paracetamol, r2=0.9991 in the range of 1-6 g/mL for Pamabrom and r2=0.9993 in the range of 250-1500 g/mL for Dicyclomine hydrochloride. The commonly used excipients and additives present in the pharmaceutical formulations were free from interfering components, as illustrated by Specificity of the method. Accuracy was performed as recovery study and results showed the...
Read More
Posted by admin on Jan 1, 2016 in |
Objective: The aim of this current research is to study a novel carrier, niosome, for its enhanced transdermal delivery of tenoxicam. Methods: Niosomes are self assembled vesicles composed primarily of synthetic surfactants, cholesterol & edge activator (surfactant). Niosomes were prepared by Organic solvent injection technique and characterized by particle size, entrapment efficiency, transmission electron microscopy (TEM), ex-vivo skin corrosive test, in-vitro dissolution, ex-vivo permeation studies and in-vivo study. Results: Vesicle size and % EE of the optimized niosomal formulation was found to be 79.46 ± 55 nm and 88.8 ± 4.5% respectively. Zeta potential of the optimized niosomal formulation was found to be -39.6 ± 7.43 mv. The ex-vivo study showed transdermal flux of niosomal gel to be 43.09± 0.106µg/cm2/h which was 2.9 fold higher than that obtained after application of plain drug gel whose flux was 15.05±0.09 µg/cm2/h). Conclusions: Data obtained from this experimental work concludes niosomal formulation is safe, very effective and promising drug carrier for transdermal delivery of...
Read More
Posted by admin on Jan 1, 2016 in |
A simple sensitive, accurate, precise and stability indicating high performance thin layer chromatographic method has been developed and validated for the estimation of troxipide in bulk drug and formulation. The method was developed using silica Gel 60 F254TLC plates, with hexane: ethyl acetate: methanol: water: ammonia 1: 5: 5: 1.5:0.2 (v/v) as mobile phase. The densitometric scanning was performed at λ = 263 nm. The method was validated for linearity, accuracy, precision, specificity and robustness according to ICH guidelines. The calibration plots were linear in the range 200-700 ng/band with r2= 0.998. The LOD and LOQ were found to be 20.39 and 61.81 ng/spot, respectively. Troxipide was subjected to acidic, alkaline hydrolysis, oxidation, photo degradation and thermal degradation. The drug undergoes degradation in acidic as well as alkaline hydrolysis and thermal degradation; however it was found to be relatively stable for photolytic and oxidative degradation conditions. As the drug peak and the degradation product peaks were well separated from each other; this method can be employed as stability indicating...
Read More
Posted by admin on Jan 1, 2016 in |
Decalepis hamiltonii Wight and Arn. is an indigenous medicinal plant, which has a folk reputation in Western and southern India as hypoglycemic agent. The present investigation was carried out to evaluate the antidiabetic effect of ethanolic seed extract of Decalepis hamiltonii in alloxan induced diabetic Albino rats. Blood glucose levels and body weights of control and diabetic rats were monitored. In the present study activities of liver enzymes such as glucokinase, glucose -6- phosphatase and fructose -1- 6-diphosphatase were also determined. Glibenclamide an antidiabetic oral drug was used as reference in the present investigation. Oral administration of ethanolic seed extract (20 mg/kg body weight) for 21 days resulted in a significant (P<0.05) decline in blood glucose from 264.03±10.07 to 90.16±2.82 mg/dl and significant recovery in body weight of diabetic rats. There was also a significant (P<0.05) reduction in the activities of glucose-6-phosphatase and fructose-1-6-disphosphatase , glutathione levels, lipid peroxidation as oxidative stress γ-Glutamyl transpeptidase, Glutathione S-transferase, aspartate aminotransferase (AST), alanine aminotransferase (ALT), acid and alkaline phosphatases in liver were...
Read More
Posted by admin on Jan 1, 2016 in |
Asparaginase is used to treat acute lymphoblastic leukemia. Acute lymphoblastic leukaemia is seen in both adults and children. It is a most common pediatric cancer, the disease is more common in ages between 2-5 years children. The causative source of acute lymphoblastic leukaemia to be multiple routes, by genetic susceptibility, endogenous exposure or exogenous exposure. L-asparaginase catalyzes the hydrolysis of L-asparagine to L-aspartic acid. L-asparaginase is commonly used as an antineoplastic agent in lymphoblastic leukaemia chemotherapy. We assumed that a structural bioinformatics approach utilizing homology-based molecular modelling and docking approaches. We used sequence data and a homology-based approach to construct a 3D-structural model of asparaginase-like protein from NCBI (GenBank: AAM28434.1). The predicted model was then used to perform molecular docking simulations with natural flavonoid derivatives to assess their ability. The ligand binding residues were found to be similar to the predicted active site...
Read More
