Posted by admin on Jan 31, 2016 in |
Bacterial diseases are highly virulent and cause significant crop loss in Anthereae mylittadrury (Daba TV).Therefore an attempt has been made to evaluate the efficient concentration of sodium hypochlorite in controlling bacterial infection through studies on mortality, Total haemocytes, Protein carbonyl content, Alanine transaminase, Aspartate transaminase in the fifth in star larvae. Results reveal that supplementation of 0.05% sodium hypochlorite concentration had significantly reduced the mortality rate due to bacterial infections. In comparison with the control (14788±123), the haemocyte count in 0.05% sodium hypochlorite supplemented larvae were recorded low as14125±146 whereas 0.1% and 0.01%concentration supplementation resulted in an increase of 15025±158 and 14965±185 haemocytes. In comparison with the control results also show that protein carbonyl content was doubled with 0.05% supplementation whereas an increase of 33.3% and 16.6% was recorded with 0.01% and 0.1% respectively. Alanine aminotransferase had shown significant increase of 138.7% with 0.05% sodium hypochlorite supplementation followed by 39% increase with 0.01% concentration in its activity. Supplementation with 0.1% did not show much variation in Alanine aminotransferase from...
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Posted by admin on Jan 31, 2016 in |
Microspheres are one of the drug delivery systems used to target drug at the desired site. The objective of the present study was to prepare, characterize and evaluate colon targeted microspheres of budesonide for the treatment of ulcerative colitis. Microspheres were prepared by ionotropic gelation process using tripolyphosphate as cross- linking agent. Budesonide containing chitosan microspheres were coated with eudragit S-100 by solvent evaporation technique to prevent drug release in the stomach. Prepared microspheres were evaluated for particle size, surface morphology, entrapment efficiency and in vitro drug release. The microspheres formed have rough surface and spherical shape as observed in scanning electron microscopy. Particle size increases with increasing concentration of polymer and entrapment efficiency ranged from 77-92%. All values are statistically significant. It was observed that the coated microspheres showed no release in the simulated gastric fluid, negligible release in the simulated intestinal fluid and maximum release in presence of rat caecal content. Budesonide microspheres prepared with chitosan show promising result in in vitro...
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Posted by admin on Jan 31, 2016 in |
Subgingival obligate anaerobes present within the biofilm such as Porphyromonas gingivalis, Tannerella forsythia, Prevotella intermedia and Aggregati bacter actinomycetemcomitans have been implicated in the initiation and progression of periodontal disease. Recent documentation has demonstrated that P.gingivalis is capable of maintaining its viability when exposed to oxidative stress by expressing Bat A and Bat B genes of the aerotolerance operon acting as virulence factors offering protection and further disease progression in the host. The present study aims to determine the presence of bat A and bat B genes in other periodontopathogenic species viz. Aggregatibacter actinomycetemcomitans, Tannerella forsythia and Prevotella intermedia, and the degree of homology using BLAST...
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Posted by admin on Jan 31, 2016 in |
The objective of the present investigation is to study the possibilities to improve the aqueous solubility and dissolution rate of Tamoxifen citrate (TC) – an oral anticancer drug, based on inclusion complexes with a– cyclodextrin (α –CD). The inclusion processes are discussed based on absorption, emission, FT-IR, 1H-NMR, SEM and phase solubility study. The α –CD study shows that the drug form 1:1 inclusion complexes. The solubility and dissolution results revealed that there was a considerable increase in solubility and dissolution of all inclusion complexes. Scanning Electron Microscopy (SEM) to analyze physicochemical interaction between drug and carrier and evaluation of the crystal morphology and their approximate size. The inclusion complex appears as tiny aggrigates of amorphous pieces of irregular sizes. In both absorption and emission the intensity increase with increase in concentration of α –CD. The aromatic ring is encapsulated in the non-popular α –CD cavity. The formation constant value reveals a hydrogen bonding interaction is present between the drug and α –CD cavity. The presence of aliphatic chains...
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Posted by admin on Jan 31, 2016 in |
The aim of this study was to investigate the potential antidiabetic activity of Piper betle in alloxen induced diabetics. Rats were divided into 6 groups and Piper betle was administered containing 50, 100 and 200 mg/kgbwt powder, respectively in 1ml water orally in group A, B and C rats. Metformin (150 mg/kgbwt) used as a reference standard drug. Blood glucose (BG), triglycerides (TG), total cholesterol (TC), high density lipoproteins (HDL), low density lipoproteins (LDL), serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) were estimated from the serum by using standard kits. Piper betle juice had shown significant lowered the blood glucose levels in all groups. In addition, body weight, organ (liver, kidney, heart and pancreas) weight, food intake, water intake were also examined in all treated groups and compared against diabetic control group. After 22 days daily administration of Piper betle, diabetic treated rats showed improvement in body weight, water intake as compared to diabetic control rats. In alloxan induced diabetic rats the maximum reduction in...
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