Posted by admin on Oct 31, 2015 in |
The core finding of the present preclinical study demonstrated that, administration of increasing doses of Agmatine is remarkably neuroprotective in male wistar rats against 6-Hydroxydopamine induced neurotoxicity. Parkinson’s disease (PD) is a chronic and progressive neurodegenerative disease with multiple motor and non-motor features that contribute to the impairment of health-related quality of life (QOL). It is associated with the loss of brown pigment neuromelanin with preferential loss of the dopaminergic neurons of the substantia nigra pars compacta results in oxidative stress and mitochondrial dysfunction. It is characterized by reduced movement, rigidity, and tremor. Agmatine (AGM) (1-Amino-4-guanidinobutane, 4 (Aminobutyl) Guanidine,1- guanidinobutane) pharmacological and electrophysiological evidence indicate that Agmatine act at N-methyl-d-aspartate (NMDAr) and nitric acid synthase (NOS) to exert anti-excitatory effects throughout the central nervous system. Agmatine act as neuroprotective agent & memory enhancer. Relevent doses of Agmatine treatment protects behavioural changes, significantly attenuated oxidative damage & improved mitochondrial complexs enzyme activities in different regions (striatum, cortex and hippocampus) of rat brain against 6-OHDA induced neurotoxicity. I.C.V. administration of 6-Hydroxydopamine is...
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Posted by admin on Oct 31, 2015 in |
Objective: The objectives are to assess the drug dose administration and reduce the dose toxicity in geriatric patients. Method: A Prospective interventional study has been carried out on drug dose administration in geriatric patients with respect to creatinine clearance rate at a tertiary care hospital for about 6 months. A total number of 204(47 baseline+157study) geriatric patients are included who has attended the general medicine ward. Results: In our study, we observed that Creatinine clearance was decreased as age increases. In study population 60-65yrs (48.6ml/min) was more, in 80-85yrs (29.2ml/min) was less and also similar in baseline study. So we can understand that geriatrics has a moderate renal failure. In a baseline among 47 subjects, 53% require dose modifications. In baseline 11 (44%) subjects experienced adverse drug reactions. Mostly Augmented types of adverse effects were observed. Among 157 study population, 28% were undergone dose modification by the suggestion of a pharmacist. It was observed that in 31 (70.4%) dose of the drug was reduced, in 6(13.6%) dose interval was...
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Posted by admin on Oct 31, 2015 in |
The manuscript devoted to investigation of some patterns of the mode of action of retabolil, compound LES-2222 (new thiopyrano[2,3-d]thiazole derivative (2-(2-Oxo-5a,11b-dihydro-5H,6H-7-oxa-1,4-dithia-3-aza-cyclopenta[c] phenanthren-3-yl)-N-(3-trifluoromethyl-phenyl)-acetamide) – synthesized at the Department of Pharmaceutical, Organic and Bioorganic Chemistry) and testosterone in rats under food deprivation. The changes of the activity of antioxidant enzymes – glutathione peroxidase (GPO) and catalase (CAT) were investigated in the different tissues under food deprivation and compounds treatment. The effects of all tested compounds were similar. The GPO activity decreased under the LES-2222 application in the liver and kidney and was increased in spleen and testes, but in the myocardium and skeletal muscle its level was normalized. Testosterone and retabolil treatment did not change the GPO activity in the liver and spleen. While, the decrease of activity level in kidney and skeletal muscle and normalisation in the myocardium and testes were detected. The changes of CAT activity under the tested compounds action were noticeable, but not statistically significant in the liver and testes. Significantly increased level of CAT activity in...
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Posted by admin on Oct 31, 2015 in |
Di-butyl phthalate (DBP) is an environmental contaminant used in the production of plastics, cosmetics and medical devices. In the present study, the effects of exposure to DBP on reproduction of female mice were investigated. Adult female mice given DBP through gastric intubation at 0 (control), 250 (low dose) and 1000 (high dose) mg / Kg BW/ day for 10 days. After treatment, body weight and ovarian weight of DBP treated mice decreased significantly at high dose while uterine weight decreased significantly both low and high dose as compared to control. Gonadosomatic index (GSI) and uterosomatic index (USI) of these mice were found significantly lower at both doses of DBP. DBP treated mice showed altered estrous cyclicity at both DBP doses as compared to control. Serum estradiol level was found significantly lower but the progesterone level was normal in DBP treated mice as compared to control. In ovary total cholesterol level was almost same in all three groups but activity of 3-β hydroxysteroid dehydrogenase (3-β HSD) was found significantly lower...
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Posted by admin on Oct 31, 2015 in |
This paper describes a simple, precise, and validated high-performance liquid chromatographic method for the simultaneous quantitative determination of Acetylcysteine and Cefixime in pharmaceutical formulation in the presence of degradation products. The separation was achieved using a Kromasil C8 reverse phase column (25X 0.46mm I.D, 5µ particle size) at room temperature with an isocratic mixture of methanol:KH2PO4 (50mM, pH 3.0) (30:70) at a flow rate of 1ml/min and detection at 228 nm. To establish stability indicating capability of the method, drug product was subjected to the stress conditions of acid, base, oxidative, hydrolytic, thermal and photolytic degradation. The degradation products were well resolved from Acetylcysteine and Cefixime. The developed method was validated as per ICH guidelines with respect to specificity, linearity, accuracy, precision and robustness. The developed methodology is an economic, time-saving, straight forward and precise assay for the determination of Acetylcysteine and Cefixime in pharmaceutical preparation. It can be readily utilized for Quality Assurance (Q.A) and Research and Development (R&D) laboratories of pharmaceutical...
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