Posted by admin on Nov 30, 2015 in |
The pineapple (Ananas comosus) is a multiple fruit belonging to the Bromeliaceae family, has a crude extract Bromelain accumulated in the stem, fruit, peel and leaves of the pineapple which shows proteolytic activity. The present study was carried out to assess the accumulation of bromelain in the different parts of the pineapple. The techniques homogenization with sodium acetate buffer, followed by filtration and centrifugation were used to extract bromelain from pineapple. The crude bromelain was then purified by ammonium sulphate salt precipitation, dialysis and finally subjected to ion exchange chromatography. Lowry’s method and gelatine digestion unit (GDU) analytical method were performed to estimate the concentration and activity of the crude enzyme and purified bromelain from the different parts The molecular weight of the purified enzyme was determined by SDS-PAGE (sodium dodecyl sulphate – polyacrylamide gel electrophoresis) analysis and found to be 25 to 27 KDa. The final step was to find out the effect of bromelain from leaves and stem of pineapple on pathogens of periodontitis. The enzyme exhibited...
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Posted by admin on Nov 30, 2015 in |
In vitro antioxidant activity test was carried out on partially purification of methanol extract of Acorus calamus. Partially purification was done to obtain ten fractions. Partial purification was carried out by using hexane: ethyl acetate solvent system. These fractions were studied for the polyphenolics and antioxidant activity. S6 –S9 fractions shows maximum radical scavenging activity, ferric reducing antioxidant potential in S3, S7 and S9 and antioxidant properties by phosphomolybdenum method higher reduction of metals observed in S1 (34.18), S4 (33.95), S5 (33.02), S6 (31.33), S7 (27.9) and S10 (35.53) mM of ascorbic acid / gm of...
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Posted by admin on Nov 30, 2015 in |
Backdrop: The design, development, formulation and in vitro evaluation of colon targeted drug delivery system with natural polysaccharides was studied with Guargum, Cyclodextrin, Amylose and Pectin. Hence it motivated us to find the combined effect of the natural polysaccharides with Celecoxib matrix tablet for specific targeting to colon. Process: Celecoxib matrix tablet was prepared by drug with equal ratio of Guargum+Cyclodextrin(A+B), Amylose+Pectin (C+D) were taken in the following equal ratio 1:0.2, 1:0.3 & 1:0.4 ratio of both gums, 2% of starch paste was added as binder. The micro crystalline cellulose was added to make the tablet total weight of 200mg.The formulations were coded as (A+B) EF1, (A+B) EF2, (A+B) EF3, (C+D) FF1, (C+D) FF2 and (C+D) FF3respectively. The preformulation studies such as Bulk density (g/cm3), Tapped density (g/cm3), Compressibility index (%), Angle of repose, Average particle size (µm) were determined. Outcome: The formulated Celecoxib Matrix tablet was evaluated for its hardness, friability, weight variation, thickness and diameter. The discharge summary of the matrix tablet was carried out in Simulated...
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Posted by admin on Nov 30, 2015 in |
Introduction: Green tea contains epigallocatechin gallate (EGCG) that widely use as antibacterial. Formulation development with buffer and emulsifier addition is needed to increase its stability and effectiveness. Buffer addition will increase EGCG stability while emulsifier will increase the cream stability. Objective: This study aimed to determine the optimal formulation from buffer combination between citric acid, ascorbat acid, and triethanolamine that produce pH 5 and also emulsifier combination from propylene glycol, tween 80 and span 80 using simplex lattice design method. Moreover, the effectiveness of optimum cream formula was tested against Propionibacterium acnes (P.acnes) and Staphylococcus epidermidis (S.epidermidis) while its sensitivity was tested on rabbit’s skin. Methods: Buffer optimization was obtained using 13 compositions with the combination of citric acid, ascorbat acid, and triethanolamine namely: 100-0-0,0-100-0,0-0-100,50-0-50,50-50-0,0-50-50,33-33-33,1,67-1,67-6,67, 6,67-1,67-1,67, 1,67-6,67-1,67, 100-0-0,0-100-0,0-0-100. Data was analyzed using design expert. The optimum buffer combination from ethyl acetate fraction of green tea leaves extract then formulated in the cream which contained various emulsifier composition. The comparison from 13 emulsifier composition of propylene glycol, tween 80, and...
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Posted by admin on Nov 30, 2015 in |
Ficus benghalensis is has been used in traditional medicinal practices such as Ayurveda, Unani, Siddha and homoeopathy. All parts of the tree such as fruits, aerial roots, bark and leaves are known to be traditionally associated with diarrhea, dysentery, menorrhagia, nervous disorders and tonic properties. However, in Ayurveda it is mainly known for the diabetes treatment.There are no reports giving a detailed profiling of phytochemicals present in the aerial roots of F. benghalensis. The study aims to evaluate its bioactive compounds and comprehend its biological activities with traditional knowledge.Methanol and ethanol extracts from the aerial roots healthy tree of F. benghalensis were prepared and their antibacterial activity against various pathogenic gram’s positive and gram’s negative bacteria were evaluated using disc diffusion assay. DPPH & FRAP bioassays were performed to establish its traditional use as antioxidants. Total phenol and total flavonoids were estimated to correlate its antioxidant potential. The chemical composition of methanol extract was accomplished by GC-MS and bioactivity of these compounds was determined. Lupenyl acetate (35.4 %), α-Amyrenyl...
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