Posted by admin on Nov 30, 2015 in |
Magnetic nanocarriers have been used for specific drug delivery to release therapeutic drugs into target cancer cells. We used bifunctional methotrexate (MTX) conjugated magnetic nanoparticles (MNPs) (~37 nm) engineered by dopamine–polyethylene glycol to targeted folate receptor (FR)-positive cancer cells. For this purpose, MTX was chemically loaded on to MNPs with N, N-dicyclohexylcarbodiimide (DCC) in the absence of NHS at 0°C. Activation process of MTX was analyzed via synthesis of different MTX methyl esters through HPLC instrument. The HPLC analysis showed that in a selective condition and in the presence of 1.1 equiv. of reactiveagent,a-carboxyl group of MTX was more active. Enzymatic release study of MTX demonstrated that the highest rate of drug release was at pH=3.8 with protease enzyme (85.12%). Also, cytotoxicity assay revealed that Fe3O4-DPA-PEG-MTX NPs were able to target over expressed FR cell line (MCF-7) but did not have any effect on FR-negative A549 cells and significantly inhibit...
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Posted by admin on Nov 30, 2015 in |
Simple, sensitive, accurate and precise spectrophotometric methods for quantitative determination of five drugs viz., Tramadol Hydrochloride (TDH) , Dobutamine (DOB), Trimetazidine (TRMZ), Terazocin (TRZ) and Esmolol (ESM) were developed. The method for each drug depends upon oxidation of drugs by Ce (IV) (Excess) and estimating the amount of un reacted Ce (IV) by Rhodamine-B dye at 557 nm. The calibration curves obeyed Beer’s law over the concentration range of 14-130μg ml-1 (TDH), 1-14 μg ml-1 (DOB), 8-120μg ml-1 (TRMZ), 16-130 μg ml-1 (TRZ), and 12-84 μg ml-1 (ESM). The methods have been validated in terms of guidelines of ICH and has been applied to the analysis of...
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Posted by admin on Nov 30, 2015 in |
The aim of the present study was to develop and validate a new, simple, selective and economical stability indicating RP-HPLC method for the quantitative determination of resveratrol in bulk drug and pharmaceutical formulations. A Box-Behnken design supported optimization was carried out to identify the optimum chromatographic conditions. The developed method was validated for linearity, range, accuracy, precision, reproducibility, LOD, LOQ and robustness as per ICH guidelines. Forced degradation studies were carried out in different stress conditions to detect degradation peak using validated method. Optimum chromatographic separation was achieved by mobile phase consisting of methanol, water and acetic acid in 69:30:1 ratio respectively. The flow rate of 1 ml min-1 with standard RT of 2.8 min was optimized in the present study. The method was linear in the concentration range of 7.5-60 µg mL-1 with a regression coefficient (R2) of 0.999. The LOD and LOQ was found to be 1.463 and 4.737 µg mL-1 respectively. Degradation study showed major decomposition of resveratrol in photolytic stress condition. The stability indicating method...
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Posted by admin on Nov 30, 2015 in |
Aim: The aim of the study is to assess effect of lifestyle modifications and implementation of DASH diet in daily life on blood pressure in hypertensive patients. Method: A prospective observational study was conducted for a period of six months from Dec- 2013 to May-2014. The data was collected by self prepared form and analyzed using descriptive statistics. Counselling on lifestyle changes and implementation of The DASH diet were given to the study population and post counseling changes were collected at visit 2. The significant difference in systolic and diastolic blood pressure was calculated by unpaired t-test. Results: A total of 186 patients were enrolled in the study, the mean (SD) age was 55.89(9.44), the percentage distribution of the study population showed that 103(55.37%) females and males 83(44.62%). The current study showed that overall effect of life style changes and implementation of the DASH diet in their daily life had a significant decrease in systolic (p<0.0001) and diastolic blood (P<0.0001) pressure of the study population. There was significant difference...
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Posted by admin on Nov 30, 2015 in |
A simple, precise, and robust spectrophotometric method for the determination of Ceftriaxone sodium has been investigated and validated. The proposed chemical reaction involved involves the reaction primary amine of Ceftriaxone sodium with nitrite solution in acidic medium to form a diazotized product followed by the reaction of the diazotized product with suitable coupling reagent to obtained a colored product. Ceftriaxone sodium has shown λmax 510nm with absorbance of 0.126. The method obeys Beer Lambert law at concentration range of 1-20ppm by showing linearity curve between absorbance and concentration. In the method detection limits was observed 0.726ppm with quantification limit of 2.42ppm and standard deviation of 0.242. In the present work an attempt was made to develop a simple, less expensive and more reliable method for the determination of Ceftriaxone sodium in pure and pharmaceutical...
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