Posted by admin on Oct 31, 2015 in |
In this study, acute Poloxamer (P-407) and chronic cholesterol and cholic suspension (CCS) models were used in rats to evaluate the antihyperlipidemic potential of methanolic extract of Gloriosasuperba(MEGS). Two different doses of 200 mg/kg and 400 mg/kg were used in acute model.Acute model was used to confirm the prophylactic antihyperlipidemic properties of extract, and further most effective dose obtained was used in chronic model. Solution of P-407 was administered into rats after 8 days of treatment with extract via i.p. (intra-peritoneal) route. In chronic model the hyperlipidemia was induced prior to treatment for 28 days with most effective dose obtained from acute model. Blood was withdrawn from retro-orbital plexus and serum lipid profile levels were estimated from both the models. In chronic model, assay of liver enzymes were estimated after scarification of rats. 400mg/kg showed the most prominent effects from acute model. MEGS reduced 51.56% of total cholesterol (TC), and 98.05% reduction of LDL level were observed in chronic model. Reduced atherogenic index was also...
Read More
Posted by admin on Oct 31, 2015 in |
Amount of moisture content present in solid dosage forms plays a major role in influencing the physicochemical properties of tablets such as dissolution rate, disintegration time, hardness, etc. The aim of the current study was to investigate the effect of relative humidity (RH) exposure on various properties of acetaminophen tablets manufactured by direct compression and wet granulation methods. Microcrystalline cellulose was used as diluent and Plasdone™ K-29/32 and Kollidon® 90F as binder in different formulations. Wet granulation end point was determined using thermal effusivity measurements. Compressed tablets were exposed to 2%, 22%, 52% and 75% RH for 48 hours at room temperature (26 ± 10C). Powder rheometer was used to evaluate the flow properties of granules after RH exposure.USP dissolution apparatus II was used to study the in-vitro drug release profiles after humidity exposure compared to reference. Thermal properties of different humidity exposed granules were examined by modulated differential scanning calorimetry. Tablets were further analyzed for hardness and disintegration time. The observed results suggested that the initial drug release...
Read More
Posted by admin on Oct 31, 2015 in |
Rapanea ferruginea Mez. stem barks and fruits extracts have demonstrated anticholinesterasic and antimicrobial activities assigned to myrsinoic acids A and B (MAA and MAB), compounds that have also proven anti-inflammatory and antinociceptive activities. To stablish the quality control of nanotechnology-based herbal medicines, the aim of this study was develop and validate a stability indicating HPLC–DAD method for quantitative analysis of MAA and MAB markers in R. ferruginea stem barks and fruits extracts and nanoemulsions. Chromatographic separation was conducted on a Kinetex®C18 column (150 x 4.6 mm x 2.6 µm), with mobile phase consisted of acetonitrile: methanol: water (acidified with H3PO4 pH 2.50) and eluted in gradient mode with flow rate of 0.9 mL min-1, with 30 min of analysis and detection at 260 nm and 270 nm. The method was specific, linear, sensitive, accurate and robust for both markers. The chromatograms of stem barks and fruits extracts submitted to forced degradation study (acid, oxidative, UV and visible light) did not show impurities interference in MAA and MAB quantification, therefore,...
Read More
Posted by admin on Oct 31, 2015 in |
Background: Hepatic encephalopathy (HE) is a brain dysfunction caused by liver insufficiency and/or portosystemic shunting. It is associated with poor survival and high risk of recurrence along with reduced quality of life (QOL) of patients and their caregivers. Nitazoxanide (NTZ) is an oral antimicrobial that improved mental status and QOL score in HE patients and is well-tolerated. Aim: To evaluate the efficacy and safety of NTZ compared to metronidazole and rifaximin in patients with grade II-III HE and to evaluate its effect on patients’ QOL. Patients and Methods: A Prospective, Randomized, Controlled, Open-Label, Pilot study. Thirty four patients were randomly assigned to receive either Nitazoxanide (n=12), Metronidazole (n=11) or Rifaximin (n=11) for 7 days. Serum ammonia level, Clinical Hepatic Encephalopathy Staging Scale (CHESS) and Chronic Liver Disease Questionnaire (CLDQ) for QOL was measured at baseline and at end of treatment. Results: Baseline and after 1 week serum ammonia levels and CHESS scores, showed no significant difference among the 3 groups. There was no significant difference in serum ammonia level...
Read More
Posted by admin on Oct 31, 2015 in |
The study was aimed to investigate the activity of hydroalcoholic extract of Cucumis sativus in gastric ulcerated rats. Anti ulcer activity was evaluated via oral administration of HACS at the dose 250, 500 and 1000mg/kg thrice in a day for 5 days before pyloric ligation ulceration, indomethacin and ethanol induced ulceration. Various parameters such as ulcer index, gastric volume, pH of gastric juice, total acidity, estimation of total proteins, total carbohydrates, total hexoses, hexosamine, fucose, salic acid and histopathological parameters were studied in the control group and pretreated groups with hydroalcoholic extract of fruit pulp of Cucumis sativus and standard drug ranitidine. In indomethacin, ethanol and pyloric ligation model, the pretreatment with HACS and ranitidine significantly reduce the ulcer index, free acidity as compared with controlled group. The % protection of ulcers were evaluated. The extract showed significantly (P<0.05) increase in pH with significant decrease in volume of gastric juice, free and total acidity. Histopathological findings also confirm the antiulcer activity of Cucumis sativus fruit pulp extract in albino...
Read More
