Posted by admin on Nov 30, 2015 in |
Context: The leaves Morus alba have been traditionally used in Asian countries for treatment of different ailments. Objective: Present study was undertaken to evaluate the effect of ethanol extract from Morus alba leaves supplementation on gastric tissue glutathione level in indomethacin induced ulcers in rats. Materials and Methods: Ethanol extract from Morus alba leaves were administered at the doses 200 and 400 mg/kg/day, p.o. for seven days. Ulceration was induced by giving indomethacin. Ulcer index, gastric GSH level, gastric wall mucus level was estimated. The functioning of liver was also assayed. Results: The effects of ethanol extract from Morus alba leaves were significant and comparable to reference treated group of rats. The gastric GSH levels were replenished by Morus alba leaves in 400 mg/kg. Conclusion: From this study it was concluded that ethanol extract from Morus alba leaves possess significant ulcer protection by replenishing gastric GSH and increasing gastric mucus...
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Posted by admin on Nov 30, 2015 in |
The present study involves the investigation of anti-inflammatory activity for 2’, 4’ dihydroxy flavone by TPA [12- O- Techanoyl 13 – Myristate] induced inflammation in Polymorphonuclear leukocytes [PMNL]. The cell viability was assayed by Trypan blue dye exclusion assay, MTT assay, release of Cathepsin D, Nitrite and TNF-α levels. Diclofenac sodium was used as the standard drug. The selected dihydroxy flavone, 2’, 4’ dihydroxy flavone showed the significant increase in the number of viable PMNL cells and decreases the release of Cathepsin D, Nitrite and TNF-α. The present work evidenced the in-vitro anti-inflammatory effect of 2’, 4’ dihydroxy...
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Posted by admin on Nov 30, 2015 in |
The antioxidant activity of pomegranate fruit was evaluated using in vitro tests. Phenol content was more in G-137, 11.8±0.11 mg/g dry wt followed Arakta variety 10.1±0.05 mg/ g dry wt., respectively flavonol and flavonoid content was high in mrudula 3.5±0.05 mg/g dry wt, 7.0±0.05 mg/g dry wt., and proanthocyanin content was high in Bhagwa variety 19.62±0.05 mg/g dry wt, Anthocyanin content and Ascorbic acid content was high in Bhagwa variety. 40.8±0.3 %, 0.95±0.05 % Total carotenoid content, and β- carotene content was high in Bhagwa variety 10±0.1 mg/g fr...
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Posted by admin on Nov 30, 2015 in |
The pineapple (Ananas comosus) is a multiple fruit belonging to the Bromeliaceae family, has a crude extract Bromelain accumulated in the stem, fruit, peel and leaves of the pineapple which shows proteolytic activity. The present study was carried out to assess the accumulation of bromelain in the different parts of the pineapple. The techniques homogenization with sodium acetate buffer, followed by filtration and centrifugation were used to extract bromelain from pineapple. The crude bromelain was then purified by ammonium sulphate salt precipitation, dialysis and finally subjected to ion exchange chromatography. Lowry’s method and gelatine digestion unit (GDU) analytical method were performed to estimate the concentration and activity of the crude enzyme and purified bromelain from the different parts The molecular weight of the purified enzyme was determined by SDS-PAGE (sodium dodecyl sulphate – polyacrylamide gel electrophoresis) analysis and found to be 25 to 27 KDa. The final step was to find out the effect of bromelain from leaves and stem of pineapple on pathogens of periodontitis. The enzyme exhibited...
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Posted by admin on Nov 30, 2015 in |
In vitro antioxidant activity test was carried out on partially purification of methanol extract of Acorus calamus. Partially purification was done to obtain ten fractions. Partial purification was carried out by using hexane: ethyl acetate solvent system. These fractions were studied for the polyphenolics and antioxidant activity. S6 –S9 fractions shows maximum radical scavenging activity, ferric reducing antioxidant potential in S3, S7 and S9 and antioxidant properties by phosphomolybdenum method higher reduction of metals observed in S1 (34.18), S4 (33.95), S5 (33.02), S6 (31.33), S7 (27.9) and S10 (35.53) mM of ascorbic acid / gm of...
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