Posted by admin on Sep 30, 2018 in |
Oxidative stress is involved in the pathogenesis of various degenerative diseases like cancer, inflammation etc. Thus, the study of antioxidant activity of natural molecules has remained a fertile ground for the researchers. In continuation of that effort, the present study was designed to evaluate the antioxidant, cytotoxic, antibacterial and anti-inflammatory potential of chloroform extract of Stereospermum chelonoides leaves (SCLC). Preliminary phytochemical analysis followed by total phenol and total flavonoid determination assays were carried out. Antioxidant potential was evaluated using DPPH radical scavenging assay, total antioxidant capacity, ferric reducing antioxidant power and cupric reducing antioxidant assays. Brine shrimp lethality bioassay and MTT based cytotoxicity assay was also conducted. Antibacterial assay was carried out using disc diffusion method on both gram-positive and gram-negative bacteria. Croton oil and xylene induced ear oedema models were used to determine anti-inflammatory activity. Phytochemical screening ensured the presence of various secondary metabolites notably polyphenols (38.04 ± 0.77 mg/g, GAE) and flavonoids (130 ± 7.86, mg/g, QE) which explained its dose dependent antioxidant activities. Moreover, cupric ion...
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Posted by admin on Sep 30, 2018 in |
The efficiency of drug discovery and designing process can be increased by effective strategies given by computational methods. Drug designing is an intense, time consuming and an interdisciplinary venture. Traditionally, drugs were discovered by synthesizing compounds in a long-drawn-out and multi-step process. Recent studies have shown an increase in day by day demand of novel and more efficient drugs. Due to availability of low-cost computer power, the use of such computers has become a leading topic in medicinal chemistry. In search of potent drugs computational techniques like docking, homology modelling, pharmaco-phore modelling, are employed by researchers around the globe in order to achieve the goal. The main purpose of this document is to give a summary of drug design process and specifically the role of computational modelling techniques. These involve some techniques from binding sites prediction to high throughput screening of large compound...
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Posted by admin on Sep 30, 2018 in |
Naturally occurring non-starch polysaccharides are known for their therapeutic value. This paper describes isolation, characterization and evaluation of glucose lowering potential of one such plant polysaccharide isolated from the leaves of the plant Vitex negundo. It was isolated and characterized using various analytical techniques and biochemical tests which confirmed the presence of carbohydrates and elements such as C, H and O. It demonstrated a reduction in glucose concentration in-vitro, however non-significant in comparison to standards. In-vivo, it was given per orally in fasting male C57 mice at 10, 30 and 100 mg/kg single dose post 30 min of which serum glucose was recorded. Glucose load was given 30 min after administering crude polysaccharide after which serum glucose concentrations were estimated at 15, 30, 60 and 120 min. Obtained results suggest a non-significant reduction in AUC as well as individual glucose levels at all the time points in crude polysaccharide treated animals in comparison to vehicle control in fasting as well as glucose loaded conditions. Thus, the crude polysaccharide isolated...
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Posted by admin on Sep 30, 2018 in |
The study carried out to find the effect of Aloe vera extract on the toxicity induced by INH – RIF in male Wistar albino rats. We have perform the experiments on rats for 30 days and found the effect on histological alternations of liver by two different method. Firstly, by Compound microscopy and the same tissues were further process through SEM microscopy. We have found the effect of Aloe vera extract and isoniazid and rifampicin on histological architecture of liver. We found that there is partial restoration of hepatic function as evident from normalization of serum markers of liver function, and we were able to show hepatoprotection against INH+RIF induced hepatotoxicity, as evidenced by the partial reversal of increased serum transaminases showed trend towards returning to normal (but partially) by supplementation of Aloe vera indicating partial hepatoprotective effect. Co-administration of Aloe vera extract along with anti-tuberculosis drugs showed hepatoprotection in some extent against INH-RIF...
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Posted by admin on Sep 30, 2018 in |
The objective of the study was to assess the effect of Pharmaceutical care training on Knowledge, Attitude and Practices of Pharmacists from various settings. In this prospective interventional study, a validated Knowledge, Attitude and Practice questionnaire was administered, before and after an educational training to the pharmacists belonging to hospital, community and corporate settings of Pune district of Maharashtra. The Post-training impact on knowledge, attitude and practices was assessed by administering the same questionnaire after 3 months to the same set of pharmacists. The results were analyzed using paired ‘t’ test and simple descriptive analysis. A total of 446 pharmacists were enrolled in the study. In the post-educational intervention, the overall Knowledge scores of participants were found to have significantly increased from 177.40 ± 59.62 to 325.8 ± 76.11; a mean score with value of p= 0.0089, a significant improvement in Attitude from 302.40 ± 82.80 to 409.80 ± 22.90; a mean score with value of p = 0.0234 and improvement in Practice component was from 149.20 ± 53.03...
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