Posted by admin on May 31, 2020 in |
Resistance to antimicrobials by pathogenic bacteria is a global menace. An attractive pathway to resolve this problem of resistance may be achieved by targeting the bacterial quorum sensing (QS) process. Fighting bacterial infections by interfering with their command language and disrupting virulence expression could serve as a viable alternative. One of the current strategies is to attenuate virulence gene expression of agr quorum sensing system of some Gram-positive bacteria through inhibition of AgrA_ P2/P3 interaction. Tertiary structures of AgrA proteins of some pathogenic bacteria like Listeria monocytogenes, Chlamydia trachomatis, Enterococcus faecalis, Streptococcus pyogenes and Macrococcus canis showing antimicrobial resistance are not yet available in structural databases like PDB. These proteins play a key role in AgrA quorum sensing. 3D structures of these proteins are essential to determine most of their functions, and for the development of agents that can be explored as therapeutic agents. In order to derive structures of these AgrA proteins, homology modeling approach was employed. The modeled proteins were validated by several methods, and the physico-chemical...
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Posted by admin on May 31, 2020 in |
Herbal medicine nowadays offers several options to modify the progress and symptoms of medicines. There has been a new trend in the preparation and marketing of drugs based on medicinal plants and their scientific and commercial significance. These plant-derived products are carefully standardized and their efficacy and safety for a specific application have been demonstrated. The objective of this work is to prepare different crude extracts from the leaves of a medicinal plant, Bacopa monnieri through the maceration method and determine their total phenolics, flavonoids and other components and also the antioxidant activity by established methods. The Bacopa monnieri leaves were collected from East Kolkata Wetland, India. The different crude extracts were prepared by using different solvents of different polarities. The total phenolic contents were determined by using the UV visible spectroscopy method. The content of the total phenolic of different leaves crude extracts was in the range of 17.43 mg/g-46.24 mg/g of dry crude extracts. The antioxidant activity was assessed by conventional a-diphenyl-b-s-picrylhydrazyl (DPPH) methods. The highest antioxidant...
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Posted by admin on May 31, 2020 in |
Cancer is the major cause of mortality in most of the developing countries. Recent times have seen an exceptional advancement in cancer biology Research. Researchers undertook the development of enormous chemotherapeutic agents. Pim family of serine/threonine kinases regulated by calcium/calmodulin have been identified as unique molecular targets in oncogenesis. It constitutes three members: Pim-1, Pim-2, and Pim-3 discovered by cloning the proviral integration site in Moloney murine leukemia virus (M-MuLV). Pim-1 was found to bear two isoforms Pim-1S and Pim-1L. It plays a dominant role in various processes of cell regulation like replicative senescence, drug resistance, apoptosis, epidynamics regulator, diagnostic tool, prostate cancer biomarker, and an immunotherapy agent. An insight into the structure of Pim kinases by crystal studies laid down a molecular basis for the development of selective and synergistic anti-cancer therapies. These proto-oncogenes are overexpressed in B lymphoid, myeloid cell lines, hematopoietic malignancies, and prostate cancer. This review emphasizes the importance and role of Pim-1 kinase in various molecular signaling pathways involved in tumorigenesis and the potential...
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Posted by admin on May 31, 2020 in |
The aim of the present study was to develop a controlled release formulation of Lornoxicam to maintain constant therapeutic levels of the drug for over 12 h. Guar gum, xantham gum, and carbopol 934 were employed as polymers. All the formulations were passed various physicochemical evaluation parameters, and they were found to be within limits. From the dissolution studies, it was evident that the formulation (F2) showed better and desired drug release patterns, i.e., 99.65% in 12 h. It contains the guar gum polymer. It followed the Higuchi order release kinetics...
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Posted by admin on May 31, 2020 in |
The development of analytical methods is in need for the estimation of drugs in pure and different pharmaceutical formulations. A simple, sensitive, rapid, accurate, precise and economic spectrophotometric and spectro-fluorimetric method was developed and validated for Nadolol in pure and pharmaceutical formulations. The proposed method was validated according to the International Conference on Harmonization (ICH) guidelines. The wave length (λmax) used for the estimation of Nadolol is 267 nm by spectrophotometry, excitation (λEx)-267 nm and emission (λEm)-300 nm by spectrofluorimetry. The linearity of the calibration curve was validated by the high values of the correlation coefficient of regression. The percentage of drugs recovered 100.37 ± 0.94% and 99.9 ± 0.59% for spectrophotometric and spectrofluorimetric methods respectively. LOD and LOQ values for Nadolol were found to be 3.531 µg/ml and 10.70 µg/ml by spectrophotometry and 0.45 µg/ml and 1.37 µg/ml by spectrofluorimetry. The developed methods are simple and suitable for the determination of Nadolol in pure and pharmaceutical...
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