Posted by admin on May 31, 2020 in |
Nitrofurantoin is a broad-spectrum bactericidal antibiotic that affects both gram-negative and gram-positive bacteria. Nitrofurantoin exhibits bacteriostatic or bactericidal effects by inhibiting the synthesis of DNA, RNA, protein, and cell wall synthesis. Nanocrystals of NFT were prepared by a cold high-pressure homogenization technique. To improve the dissolution rate, the drug was formulated in a nanocrystal by using an emulsion diffusion method, high-pressure homo-genization or sonication. NFT was dispersed in aqueous surfactant solution containing poloxamer 188, PVPK 30, and HPMC E3 under continuous stirring. Poloxamer 188 was used as a surfactant for the preparation of the NCs. Formulation NC9B3 has a mean particle size 231 ± 9 nm with polydispersity index 0.09 ± 0.02, which indicates a very narrow particle size distribution. % Entrapment efficiency was 98.3 ± 0.7 slow drug release profile indicates the homogeneous dispersion of NFT in the lipid matrix. NCs have crystalline nature with rough surfaces, which has been confirmed using SEM analysis. XRPD spectra show the reduction in the crystalline behavior of the drug and the...
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Posted by admin on May 31, 2020 in |
Resistance to antimicrobials by pathogenic bacteria is a global menace. An attractive pathway to resolve this problem of resistance may be achieved by targeting the bacterial quorum sensing (QS) process. Fighting bacterial infections by interfering with their command language and disrupting virulence expression could serve as a viable alternative. One of the current strategies is to attenuate virulence gene expression of agr quorum sensing system of some Gram-positive bacteria through inhibition of AgrA_ P2/P3 interaction. Tertiary structures of AgrA proteins of some pathogenic bacteria like Listeria monocytogenes, Chlamydia trachomatis, Enterococcus faecalis, Streptococcus pyogenes and Macrococcus canis showing antimicrobial resistance are not yet available in structural databases like PDB. These proteins play a key role in AgrA quorum sensing. 3D structures of these proteins are essential to determine most of their functions, and for the development of agents that can be explored as therapeutic agents. In order to derive structures of these AgrA proteins, homology modeling approach was employed. The modeled proteins were validated by several methods, and the physico-chemical...
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Posted by admin on May 31, 2020 in |
Herbal medicine nowadays offers several options to modify the progress and symptoms of medicines. There has been a new trend in the preparation and marketing of drugs based on medicinal plants and their scientific and commercial significance. These plant-derived products are carefully standardized and their efficacy and safety for a specific application have been demonstrated. The objective of this work is to prepare different crude extracts from the leaves of a medicinal plant, Bacopa monnieri through the maceration method and determine their total phenolics, flavonoids and other components and also the antioxidant activity by established methods. The Bacopa monnieri leaves were collected from East Kolkata Wetland, India. The different crude extracts were prepared by using different solvents of different polarities. The total phenolic contents were determined by using the UV visible spectroscopy method. The content of the total phenolic of different leaves crude extracts was in the range of 17.43 mg/g-46.24 mg/g of dry crude extracts. The antioxidant activity was assessed by conventional a-diphenyl-b-s-picrylhydrazyl (DPPH) methods. The highest antioxidant...
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Posted by admin on May 31, 2020 in |
Cancer is the major cause of mortality in most of the developing countries. Recent times have seen an exceptional advancement in cancer biology Research. Researchers undertook the development of enormous chemotherapeutic agents. Pim family of serine/threonine kinases regulated by calcium/calmodulin have been identified as unique molecular targets in oncogenesis. It constitutes three members: Pim-1, Pim-2, and Pim-3 discovered by cloning the proviral integration site in Moloney murine leukemia virus (M-MuLV). Pim-1 was found to bear two isoforms Pim-1S and Pim-1L. It plays a dominant role in various processes of cell regulation like replicative senescence, drug resistance, apoptosis, epidynamics regulator, diagnostic tool, prostate cancer biomarker, and an immunotherapy agent. An insight into the structure of Pim kinases by crystal studies laid down a molecular basis for the development of selective and synergistic anti-cancer therapies. These proto-oncogenes are overexpressed in B lymphoid, myeloid cell lines, hematopoietic malignancies, and prostate cancer. This review emphasizes the importance and role of Pim-1 kinase in various molecular signaling pathways involved in tumorigenesis and the potential...
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Posted by admin on May 31, 2020 in |
The aim of the present study was to develop a controlled release formulation of Lornoxicam to maintain constant therapeutic levels of the drug for over 12 h. Guar gum, xantham gum, and carbopol 934 were employed as polymers. All the formulations were passed various physicochemical evaluation parameters, and they were found to be within limits. From the dissolution studies, it was evident that the formulation (F2) showed better and desired drug release patterns, i.e., 99.65% in 12 h. It contains the guar gum polymer. It followed the Higuchi order release kinetics...
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