Posted by admin on Jul 1, 2012 in |
Napoleonaea imperialis is used to treat wounds in most parts of Nigeria. Against this background, N-hexane extract of the leaves were screened against some microorganisms to ascertain this claim and to recommend it for further investigation for possible inclusion into official compendium. The plant leaves were dried, powdered and extracted by cold maceration with N-hexane for 24hours. Phytochemical screening was done for alkaloids, saponin, steroidal nucleus, simple sugar, starch, glycoside, proteins and flavonoid using standard procedures. Antimicrobial screenings were carried out using agar diffusion technique. Antibacterial activity was conducted by screening against six pathogens comprising both Gram positive (B. subtilis and S. aureus) and Gram negative bacteria (P. aeruginosa, Klebsiella, E. coli and S. typhi) obtained from pharmaceutical Microbiology laboratory stock. The extract was screened against 24hour broth culture of bacteria seeded in the nutrient agar at concentrations 400, 200, 100, 50, 25, 12.5, 6.25and 3.125 mg/ml in DMSO and incubated at 370C, for 24 hours and measuring the inhibition zone diameter – IZD. The positive controls were ampicillin...
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Posted by admin on Jul 1, 2012 in |
Authorspropose a method to determine the contamination caused during the storage of colchicine stock solution, which will be helpful for the bioanalytical scientists. The process of identification of impurities, exceptionally in this case, started from the point of optimization of the compound, where in the parent ion was not detected and instead an unknown mass is being interpreted by the detector, which was further identified as a plasticizer but not as colchicine. To reconfirm the contamination by the plasticizer, a commercially available colchicine tablet was powdered and extracted with methanol and infused in Q1 MS scan mode. The supernatant solution of Colchicine extract has resulted in a transition of 400.41 m/z which was found to be more predominant. Again the tuning solution of working standard was infused with the same parameters, the same contamination parent peak was found and in addition to this experiment, confirmation against the product ion was also performed, where in the mass transition is found to be 149.177 m/z. Finally, it was found to be...
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Posted by admin on Jul 1, 2012 in |
Argemone mexicana isan indigenous herb commonly known as Prickly poppy. It belongs to the family Papaveraceae. Argemone mexicana is noted to possess medicinal benefits in traditional system of medicine. During last few decades, there has been increasing interest in the study of medicinal properties of this plant and it is reported for Antimicrobial, Antidiabetic, Antioxidant and Hepatoprotective activity. The plant was also reported for other activities like Larvicidal activity, Wound healing activity, Cancer activity, Antihelmintic activity and Neuropharmacological studies. In light of these medicinal properties, this plant can be represented as a valuable source of medicinal compound. This study depicts the overall information about the ethnobotany, phytochemistry and pharmacological activities of the A....
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Posted by admin on Jul 1, 2012 in |
Ocular inserts are sterile preparations, with a thin, multilayered, drug-impregnated, solid or semisolid consistency devices placed into cul-de-sac or conjunctiva sac. They are usually made up of polymeric vehicle containing drug. Ocular drug delivery is one of the most fascinating and challenging tasks being faced by the Pharmaceutical researchers. One of the major barriers of ocular medication is to obtain and maintain a therapeutic level at the site of action for prolonged period of time. The therapeutic efficacy of an ocular drug can be greatly improved by prolonging its contact with the corneal surface. Newer ocular drug delivery systems are being explored to develop extended duration and controlled release strategy. Some of the newer, sensitive and successful Ocular delivery systems like inserts, biodegradable polymeric systems, and collagen shields are being developed in order to attain better ocular bioavailability and sustained action of ocular...
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Posted by admin on Jul 1, 2012 in |
Topical administration of a drug in the conjunctival cul-de-sac is the treatment of choice for diseases of the anterior segment of eye. Development of ophthalmic drug delivery systems has always been challenging because of the drawbacks with this route, like non-productive absorption, drainage, induced lacrimation, tear turn over, impermeability of drugs to cornea. New approaches have been investigated for delivery of drugs to the eye by means of polymeric delivery of ophthalmic drugs to the pre-and intra ocular tissues, have been attempted to increase the bioavailability and the duration of therapeutic action of ocular drug. Certain new approaches to increase the ocular bioavailability, duration of the drug action and to reduce the undesirable side effects are by using drug carriers that regulate pre-corneal drug loss and improve the corneal contact time. Many of these systems prolong ocular bioavailability but do not control drug penetration through the cornea. Consequently, the drug concentration at the site of action might remain inadequate. Therefore, it is necessary to develop safer, efficacious and more...
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