Posted by admin on Aug 1, 2013 in |
Evaluation of the anti-hepatotoxic activity of methanol-dichloromethane (MDM) extract of Oroxylum indicum (OI) whole plant on carbon tetrachloride (CCl4)- induced hepatotoxicity in rat liver explant cultures (in-vitro) and chronic liver damage in rats (in-vivo). In toxicant (CCl4) treated explants culture set (in-vitro), the marker enzymes GOT, GPT and ALP level increased significantly (p<0.001) when compared to control set. Treatment of CCl4-intoxicated liver explant culture sets with MDM extract of OI whole plant at a concentration of 3.3 mg/ml attenuated the marker enzymes GOT, GPT and ALP levels in culture supernatants significantly (p<0.001) as compared to CCl4 treated explant culture sets. A significant hepatocellular damage as evident from significant elevation in serum activities of GOT, GPT, ALP, bilirubin concentration (total and direct) with decrease in albumin and total protein were found in CCl4: liquid paraffin (1:1) treated animals (in-vivo) when compared to normal values. The MDM extract of OI whole plant (200 mg/kg/day, p.o.) exhibited a significant reduction (p<0.001) in CCl4: liquid paraffin (1:1) induced increased serum levels of GOT,...
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Posted by admin on Aug 1, 2013 in |
With the advent of newer technologies, in expert opinions, nanobiotechnology is expected to revolutionize or at least significantly improve the pharmaceutical and life science market within the next 15 years. The problem associated with reproducibility prevailed, a need for an economical polymer is experienced by all the researchers so as to repeat and check the reproducibility of systems. To meet all these requirements, Gelatin comes in front as the best alternate. Gelatin (or gelatin, from Latin: gelatus = stiff, frozen) is a translucent, colorless, brittle (when dry), flavorless solid, derived from collagen obtained from various animal by-products. It is commonly used as a gelling agent in food, pharmaceuticals, photography, and cosmetic manufacturing. Substances containing gelatin or functioning in a similar way are called gelatinous. Gelatin is an irreversibly hydrolyzed form of collagen. Gelatin being biodegradable in nature is a polymer of choice for the delivery of various proteins and peptides, can be an efficient and safe vaccine adjuvant,can be used forin vitro delivery of immunogenic CpG oligonucleotides, can be...
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Posted by admin on Aug 1, 2013 in |
The fruit of Phoebe cooperiana U.N Kanjilal ex A. Das is highly priced and one of the most preferred folk food among the Indigenous people of Arunachal Pradesh, India. The methanol extract of fruit collected from East Siang District of Arunachal Pradesh, India were evaluated for total phenolic content, total flavonoid content and antioxidant potential. The methanol extract of fruit were subjected to 2, 2-Diphenyl-1-picrylhydazyl (DPPH) assay for free radical scavenging property and ABTS radical cation scavenging activity to determine antioxidant potential. Folin-Ciocalteu’s method for quantification of total phenolic content and colorimetric method for quantification of total flavonoid content were used in the experiment. Ethnobotany, mode of cooking, demand of fruit in the local market and unscientific harvest from forest and resultant threat on the plant is discussed in the paper. The methanol extract contains considerable phenolic (33.93±1.14 mg GAE/g) and flavonoid (941.5 ±5.31 µMRE/g) but no significant correlation between the total content of phenolic and flavonoid and antioxidant potential was found as IC50 value of 94.6±1.35µg/ml was found ...
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Posted by admin on Aug 1, 2013 in |
The icaADBC gene was first identified in Staphylococcus epidermidis, and is also present in Staphylococcus aureus and other Staphylococcal species. PIA is produced by the gene products encoded by the icaADBC operon. Asymptomatically colonized patients and health care workers are the major sources of MRSA in the hospital environment. MRSA-infected patients in burns units are particularly problematic because the big surface area of denuded skin can produce a large inoculum of organisms that can be easily transmitted to other patients via the hands of health care workers. Extensive skin lesions also result in heavy shedders of MRSA. Clinical isolates of Staphylococcus aureus can express the icaADBC-encoded polysaccharide intercellular adhesin/poly-N–acetylglucosamine (PIA/ PNAG). The icaADBC dependent and independent pathways will be stimulated using different chemicals and level of biofilm formation as well as PIA/PNAG level will be assayed. Besides, proteomics and transcriptomics analysis will be performed to get insights in the interaction of various factors of the pathways involved in the biofilm formation in wild type as well as mutant strains. The biofilm development...
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Posted by admin on Aug 1, 2013 in |
Air pollution tolerance index was assessed with plant species located at ChinnaTirupathi, Salem, Tamil Nadu, India. This study was conducted during March, 2013. Since, all the lands are converted to buildings, it is essential to know more about plants and its benefits offered to the environment and the dangers, risks caused by cutting trees. In this study, our aim is to evaluate, whether the plant is toleranant or sensitive to pollution. From the results obtained, we can infer that all the plants collected were having APTI value in the range of 9.41 to 15.46. Selected plants having APTI score of less than 16 are grouped under plants sensitive to pollution and are biological indicators of air pollution. Sensitivity of certain plants to air pollutants is variable from place to...
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