Posted by admin on Sep 1, 2014 in |
Bryophytes, a small group of lower plant phylogenetically placed between algae and the vascular plants comprise of hornworts, liverworts, and mosses. They are the second largest group of land plants and extremely rich in a variety of biologically active compounds viz. terpenoids, phenols, glycosides, and fatty acids. This small slow-growing group of plants is stockroom of naturally occurring materials and have been investigated for the antimicrobial, antioxidant, anti-inflammatory, anti-venomous, and anti-leukemic activity. In recent years, bryophytes have emerged as a potential biopharming tool for the production of complex biopharmaceuticals. Even though bryophytes could be used in medicine, the use of bryophytes for applied research with implications for human health is still not fully explored. Investigations are hindered commonly because of minute size and difficulties in identifying diverse species of bryophytes. In the present review, we focused on therapeutic uses of bryophytes in detail that will widely open the door for the use of different bryophytes in plant biotechnology and to meet the demand of novel drug...
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Posted by admin on Sep 1, 2014 in |
Coumarin (2H-1-benzopyran-2-one) nucleus and it’s natural, as well as synthetic derivatives, has been a field of interest due to its unique tendency to adopt varying categories of substitutions which provides the basis for modifying this nucleus for multidisciplinary actions. The extensive literature review reflects that coumarin derivatives have been synthesized by several structural modifications and exhibit numerous activities like anti-inflammatory, anti-tumor, anti-HIV, anticoagulant, antimicrobial, antioxidant, anti-hepatitis, hepatoprotective, anti-pyretic, analgesics, anti-thrombotic, vasodilatory, anti-mutagenic, antioxidant, anti-fungal, anti-viral, anti-hypertensive, anti-tubercular, anti-convulsant, anti-adipogenic and anti-hyperglycemic. They also inhibit lipooxygenase, cyclooxygenase, MAO-A, MAO-B, and TNF-alpha enzymes. The purpose of this study is to review the anti-inflammatory potentials of coumarins and to generate a comparative SAR that may support to improve their anti-inflammatory activity by newer and potent diverse structural modifications of the coumarin nucleus. The review shows that wide varieties of coumarin derivatives have been synthesized and have shown anti-inflammatory potentials. These derivatives can be further modified with the help of SAR generated in the present work to improve the anti-inflammatory potentials of the...
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Posted by admin on Sep 1, 2014 in |
Objective: To investigate the cardioprotective effect of methanolic extract of Gardenia gummifera root (MEGG) on Isoproterenol (ISO) induced myocardial infarction (MI) in rats. Methods: LCMS analysis of MEGG was done for the identification of cardioprotective constituents. Myocardial infarction was induced by the subcutaneous injection of ISO (6mg/100g body weight) at an interval of 24 h for 2 days. MEGG (125 and 250 mg/kg, p.o) was given to rats once daily for 45 days before the ISO challenge. The myocardial damage was assessed by quantifying the serum levels of cardiac marker enzymes (LDH, AST, ALT, CK-MB), serum iron and iron binding capacity, uric acid, and ceruloplasmin. Antioxidants such as catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione-S-transferase (GST) and reduced glutathione (GSH) were altered in MI rats. The level of lipid peroxidation was measured as malondialdehyde (MDA), triphenyl tetrazolium chloride (TTC) test, and quantification of histopathological changes also supported the dose-dependent protective effects of MEGG. Results: MEGG significantly (p ≤ 0.05) protected the above-mentioned parameters from falling from normal...
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Posted by admin on Sep 1, 2014 in |
The present study is a simple rapid, sensitive, selective and reproducible RP-HPLC method has been developed for the estimation of Triclosan in dental formulations. In present work a simple, sensitive and specific method (RP-HPLC assay, stability indicating RP-HPLC) has been developed by using a phenomenex, BDS C18, column having 5 µm particle size and 150 mm × 4.6 mm in length and gradient mode, with mobile phase containing acetic acid buffer (pH 2.5, adjusted with formic acid) and acetonitrile in the ratio of 30:70. The flow rate was 1.2 mL/min and effluents were monitored by PDA detector at 281nm. Injection volume was 20 µL. The method is linear (1-50 µg/mL). The regression equation was y = 7311x-2307 (r2 = 0.997). The method was validated for linearity, precision, accuracy, ruggedness, and forced degradation studies were performed. Estimation of triclosan was done in 3 dental formulations. Recoveries from formulations were between 98% and 102%. Limit of detection and limit of quantification was 0.101433 and 0.3333µg/mL, respectively. All the validation parameters are...
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Posted by admin on Sep 1, 2014 in |
Subcutaneous implantation of the drug is known to be the first medical approach aiming to achieve prolonged and continuous administration of drugs. The purpose of the research was to achieve sustained delivery of Tramadol Hydrochloride from biodegradable Gelatin-Sodium Alginate polymeric implant. Implants were prepared by using Gelatin-Sodium Alginate polymer in two ratios 70:30 & 80:20% w/w by heating and congealing method and then exposed to formaldehyde vapor for different periods (3, 6, 12 & 24 h) for hardening. Implants formulated with 80:20 Gelatin-Sodium Alginate ratio and hardened for 12 h were chosen for further studies based on drug loading and release performance. Effects of different excipients were studied on drug loading efficiency and drug release profile. Morphology of implant matrices, as studied by SEM, supported the experimental results. The release kinetics of drug was evaluated by fitting the data in four different kinetic models, namely, Zero order, First order, Higuchi and Korsmeyer‐Peppas. Implants were found to follow Korsmeyer Peppas Model the best in most cases. Good correlations were obtained...
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