Posted by admin on Aug 31, 2025 in |
At present, the significant cause of chronic kidney disease (CKD) is said to be Diabetic Kidney Disease (DKD). This condition considerably impacts cost of healthcare, mortality and morbidity worldwide. Current treatment options for DKD basically focusses on Blood Pressure (BP) regulation, glycemic control along with suppression of Renin-Angiotensin-Aldosterone System (RAAS). The advancing nature of pathophysiology of DKD demands additional treatment options to reduce the ongoing chronic inflammation and oxidative stress as they are the key operators of renal dysfunction. In the current review, we put forward a novel, rationally designed antioxidant-Dapagliflozin combination as a single tablet formulation incorporating N-Acetyl Cysteine (NAC), Taurine, Alpha-Lipoic Acid (ALA) and Coenzyme Q10 (CoQ10) with Dapagliflozin to boost renoprotection in DKD. In this review, we have explored the pathophysiological rationale underlying this novel drug combination, its mechanism, synergistic potential, efficacy in preventing development of DKD at the cellular level and challenges in drug formulation. This is the first article to propose this novel combination by incorporating SGLT-2 inhibitor with antioxidants based on scientific rationale...
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Posted by admin on Aug 31, 2025 in |
Background: Pharmacology education has evolved with the introduction of competency-based medical education (CBME). Traditional live animal experiments in pharmacology practical face ethical, logistical, and regulatory challenges. Computer-assisted learning (CAL) using animal simulation software offers an alternative that aligns with CPCSEA guidelines, emphasizing the 4Rs (Reduction, Refinement, Replacement, and Rehabilitation). Objectives: This study aims to evaluate the effectiveness of CAL as a teaching-learning and assessment tool for 2nd MBBS students and to analyze student feedback on the use of CAL software in pharmacology practical. Methods: A prospective observational study was conducted among 125 2nd MBBS students at Tezpur Medical College. Students participated in a CAL session on the “Effect of drugs on the rabbit eye”. Pre-test and Post-test MCQs scores were recorded. Assessment was conducted using CAL software in an examination mode. Feedback was collected using a structured questionnaire based on the Likert scale. Data analysis was done. Results: The mean pre-test score was 36%, while the post-test score improved significantly to 71% (p<0.05). The proportion of students scoring between...
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Posted by admin on Aug 31, 2025 in |
Bioanalysis is most widely used for drug discovery and development. Bioanalytical methods have been working for the quantitative estimation of drugs, or its metabolites and biomarkers in its biological samples. The steps involved in bioanalysis comprise of many steps that are from sample collection to sample analysis and then data reporting. The first step involves sample collection from clinical or preclinical studies. After that, the analysis of the sample is done in the laboratory. The second step involves the sample cleanup the cleanup process should be carried out carefully to eliminate the interferences present in the sample matrix. And it increases the performance of the analytical system. The last step involves sample analysis and detection of a separated compound and detection of techniques used are HPLC and Liquid chromatography coupled with double mass spectrometry (LCMS-MS) and are used in the bioanalysis of drugs in the body. Each one of these has its advantage and disadvantage. For bioanalysis of small or large molecules the chromatographic methods used are HPLC, and...
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Posted by admin on Aug 31, 2024 in |
Ovarian cancer, a major concern in India, is among the leading causes of cancer-related deaths in women. Early detection is crucial due to frequent late-stage diagnoses. Red Cell Distribution Width (RDW), a marker for red blood cell size variability, is promising but lacks extensive study in ovarian cancer. This research aims to correlate RDW with hematological parameters in ovarian cancer patients, potentially improving early detection and prognosis. This study examined data from ovarian cancer patients at Mc Gann’s Teaching District Hospital in Shivamogga. Hematological parameters, RDW, were obtained from records, with imaging and histopathological analysis confirming diagnoses. Inclusion criteria Involved ovarian malignancies or benign tumors, while exclusions involved comorbidities or recent treatments impacting blood parameters. Significant differences were observed in RDW (p = 0.033), Platelet-to-Lymphocyte Ratio (PLR) (p = 0.004), Neutrophil-to-Lymphocyte Ratio (NLR) (p = 0.001), Lymphocyte count (L) (p = 0.0006), and Neutrophil count (N) (p = 0.003) among the case, control, and normal groups. Post hoc analysis revealed significant disparities between the case and control groups for...
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Posted by admin on Aug 31, 2024 in |
Silymarin, an intricate blend of flavonolignans obtained from Silybum marianum, or milk thistle, has drawn a lot of interest because of its possible medical uses. This study reports on the development and validation of an analytical technique that employs high performance thin layer chromatography (HPTLC) to estimate silymarin in pharmaceutical dosage forms (tablet) and bulk drug. Chloroform: methanol (6:4 v/v) was the solvent system used in the chromatographic separation of the medication on aluminium plates pre-coated with silica gel 60 GF254, (10 cm × 10 cm with 250 μm layer thickness) as the stationary phase. At 288 nm, a densitometric analysis of the divided zones was carried out. Densitometric scanning was carried out at 288 nm using a Camag TLC scanner 3, which was controlled by Win CATS software. The radiation source utilised for the scan was a deuterium lamp with slit size of 5.00 x 0.45 mm. It was discovered that the retention factor was 0.42. In the 200–1200 ng/band range, linearity R2= 0.993 was used to evaluate...
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