Posted by admin on Sep 1, 2014 in |
The objective of the present study is to develop and validate a method for the estimation of gatifloxacin (GTX) in the tablet dosage form. An isocratic reversed-phase high-performance liquid chromatographic (HPLC) method was developed to estimate gatifloxacin using X terra C18 column (4.6 mm ´ 150 mm I.D., 5µm particle size) and the mobile phase containing phosphate buffer (pH adjusted to 3.5 with dilute phosphoric acid) and acetonitrile (60:40 v/v) at the flow rate of 0.5 ml/min. The UV detection was carried out at 298 nm. The retention time of gatifloxacin was found to be 2.340 min. The developed method was validated concerning accuracy, precession, linearity, selectivity, ruggedness, and robustness. Linearity for gatifloxacin was observed in the concentration range 0.01 to 0.50 µg/ml. Percent recovery was found to be 99.67. The results confirmed that the proposed method is specific, rapid, reproducible, and suitable for the routine determination of...
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Posted by admin on Sep 1, 2014 in |
Swertia chirayita (SC) has been traditionally used for the treatment of several metabolic disorders related to health problems. We have investigated the antidyslipidemic and antioxidant potential of Swertia chirayita (SC) plant, a potent antidiabetic, and its isolated markers. The LC-ESI-MS standardized hot water extracts of different batches from the whole plant of Swertia chirayita, were tested in-vivo for their antidyslipidemic activity in Triton (WR-1339) and high-fat diet (HFD) induced dyslipidemic Charles Foster rats and in vitro for their antioxidant activity. Further solvent fractionation and isolation resulted in the identification of antidyslipidemic Mangiferin (MG) and Amarogentin (AM). Treatment of hyperlipidemic rats with MG and AM at 100 and 200mg/kg doses caused reduction in plasma levels of Low density lipoprotein (LDL) 24, 26% and very low – density lipoprotein (VLDL) 19, 25% as well as increase in levels of High density lipoprotein (HDL) 18, 22% respectively. In rats with HFD, MG and AM significantly reduced levels of VLDL lipids (26% and 27%) as well as Low-density lipoprotein-Total cholesterol (LDL-TC) 27% and...
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Posted by admin on Sep 1, 2013 in |
Emesis is a clinical condition which requires immediate treatment. The present work is aimed to formulate Orodispersible Tablet (ODT) of BCS class II drug Domperidone using Kyron T-314 as superdisintegrant and Avicel 102 as disintegrant by direct compression method. The drug-excipients compatibility study was carried out by Fourier Transform Infrared (FTIR) and Differential Scanning Calorimetry (DSC) which reveals no interaction between drug and excipients. A 32 factorial design was used to investigate effect of independent variables viz. superdisintegrant (Kyron T-314) and disintegrant (Avicel 102) on dependent responses like friability, disintegration time and percent drug release. Sodium lauryl sulphate (SLS) was used in the formulation to aid the dissolution of drug. All the formulations were evaluated for hardness, friability, disintegration time and dissolution rate. The factorial batch F4 containing 1.5 mg Kyron T-314 and 5 mg Avicel 102 has shown better results for all evaluation parameters with drug release of 99.22% and disintegration time of 29 sec. Therefore, factorial batch F4 was selected as the optimized...
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Posted by admin on Sep 1, 2013 in |
Kamrup District of Asasm is bounded by Udalguri and Baksa districts in the north, Meghalaya in the south, Darrang and Kamrup Metropolitan in the east and Goalpara and Nalbari district in the west. The rural part of kamrup district is mostly inhabitated by different tribes such as Boro, Khoronia Kochari, Rajbonghi, and Nepalis. An ethno botanical survey was conducted during the year 2011-2012 in some selected villages to explore the medicinal plants used by the local traditional healers known as ‘bej’ and ‘Ohjha’ for treatment of diabetes mellitus. The data’s were collected using questionnaire and personal interviews with few local healers. The investigation revealed 56 species of plants belonging to 38 families which are used to cure diabetes. These traditional ethno medicinal plants are consumed either in form of juice, powder, or boiled extract of leave, stem, root, seed, fruit, bark, rhizome and flower. In some cases it was found that the whole plant is directly used as potent...
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Posted by admin on Sep 1, 2013 in |
The present study was undertaken to know about the free radical scavenging activity of plants located near Thermal power plant, Mettur, Salem. In this study, Polyalthia longifolia, Derris indica, Holopetela integrifolia, Tamarindus indica, Terminalia tomentosa, Psidium guajava, Nerium indicum, Azadirachta indica, Calotropis gigantea, Ricinus communis, Carica papaya, Ficus benghalensis, Embilica officinalis, Tectona grandis, Ficus religosa, Mangifera indica, Moringa oleifera, Acacia Arabica, Opuntia ficus indica are the plants that were collected during March to April 2013. Among the plants selected, Holopetela integrifolia, Mangifera indica, Tamarindus indica, Acacia arabica, Azadirachta indica were found to have high flavonoid content. Total antioxidant activity was measured in terms of phospho-molybdenum activity was high in Carica papaya when compared to the other entire antioxidant assay, Acacia arabica showed maximum ability to chelate metal...
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