Posted by admin on Sep 1, 2012 in |
In the present study, the microspheres containing Verapamil hydrochloride xanthan complexes encoated with ethyl cellulose by emulsion solvent evaporation method. DSC shown absence of any new endothermic peak, disappearance of no shift of endothermic peak confirmed that there is no any interaction between drug, excipients and the drug was thermally stable. Drug polymer ratio was altered while the other formulation parameters were kept constant and percentage yield, incorporation efficiency, particle size and distribution of the microcapsules were analyzed. The microcapsules studied for effects of the increase polymer ratio in formulation. The dispersed phase viscosities were evaluated by comparing with variations in particle size and distribution of the microcapsules and the effect of the variation in polymer ratio on drug dissolution was evaluated. The percentage drug released at the end of 12th h (VXM2) was found be 95.95%. respectively. The mechanism of drug release may also be dissolution and diffusion mechanism controlled released formulation. The stability of Verapamil hydrochloride loaded microcapsules (VXM2) formulation was stored at 40oC±2oC/75% RH ±5% RH...
Read More
Posted by admin on Sep 1, 2012 in |
The purpose of present investigation was to achieve successful delivery especially to colon using microbially triggered, osmotically controlled approach. Prednisolone was chosen model drug for treatment of inflammatory bowel disease (IBD). Prednisolone-β-cyclodextrin complex were prepared and phase solubility study was carried out. Cellulose acetate solution containing chitosan was used to prepare semi-permeable. Face centered central composite design was employed to study the effect on independent variables (concentration of sodium chloride, polyethylene glycol, and chitosan), % cumulative release and disintegration time. Both independent variables, the proportion of chitosan (X1) and proportion of NaCl and PEG (X2) had an influence on the % drug release in both the media (buffers and buffer with rat ceacal content). Formulations were evaluated for their Preformulation parameters and found within the specified limit. Formulation code F8 and S8 were the optimized batches with an in vitro release of 83.77 and 99.13 % respectively. The formulations containing PEG was found to be a promising drug delivery system better release...
Read More
Posted by admin on Sep 1, 2012 in |
Oxidative stress has been implicated in the eiteology of diabetic neuropathy (DN) and anti-oxidants have shown to ameliorate this condition. Previous studies have indicated that early treatment with vitamin E acetate (VE) and methylcobalamin (MCA) attenuated surgery induced neuropathy in rats. The aim of this study was to test the effect of early co-administration of VE (50 mg/kg) and MCA (500µg/kg) (VEM) on the development of DN in laboratory rats. Male Wistar rats were divided into the three treatment groups, sham (non-diabetic, vehicle treated), diabetic rats (vehicle treated; (DU), and diabetic rats which received VEM treatment (DVEM). Diabetes was induced by single injection of alloxan monohydrate (120 mg/kg). Body weights of all subjects were noted daily and serum glucose levels (SGL) were measured at regular intervals. Behavioural assessment of thermal hyperalgesia (TH) was performed using the hot plate test and warm water tail immersion test. Finally, motor nerve conduction velocities (MNCV) were measured on day 45. Significant enhancements in SGL were noted for DU rats and this remained throughout...
Read More
Posted by admin on Sep 1, 2012 in |
A simple, precise and stability-indicating HPLC method was developed and validated for the simultaneous determination of anti-diabetic drugs Metformin hydrochloride (MT), Rosiglitazone (RT) and Sitagliptin (ST). The separation was achieved on ACE 3 150 mm X 4.6mm, 3.5µm column using gradient method. The mobile phase at a flow rate of 1.5 mL min−1 consisted of 10 mM sodium hexane sulphonate monohydrate and 10 mM Potassium dihydrogen phosphate buffer with acetonitrile and methanol in Gradient ratio. The UV detection was carried out at 210 nm. The forced degradation for these drug substances were performed under Acid, Base, Oxidative, Photolytic and Thermal stress conditions. The method was successfully validated in accordance to ICH guidelines. Further, the validated method was applied for commercially available pharmaceutical dosage...
Read More
Posted by admin on Sep 1, 2012 in |
The ethanolic extracts of aerial part of Ocimum canum (OC) were studied for its antioxidant activity and its role to prevent renal ischemia. The in vitro antioxidant models used were DPPH radical scavenging activity, Hydroxyl peroxide radical scavenging method, reducing power assay & FRAP assay. The study was carried out at different concentration (250, 500, 1000, 2000 µg/ml). Further, the antioxidant activity was studied by using in vivo method to prove its potency in preventing ischemia by incorporating renal ischemia/reperfusion model in Wistar albino rats. The animals were divided into four different groups of six rats in each group. Group-1 was served as Control and received oral saline only once daily for 28 days. Group-2 received (oral saline + RI). Group 3 received Ocimum canum ethanolic leaf extract 300mg/kg bwt dose orally for 28 days, Group-4 were pretreated with Ocimum canum ethanolic leaf extract an oral dose of 100mg/kg bwt for 28 days. After the experimental period all rats were sacrificed and antioxidant defense system and oxidative stress in...
Read More
