Posted by admin on Aug 31, 2021 in |
Context: Drug-drug interactions (DDIs) are frequently observed in hospitalized cardiac patients and associated with alteration in the effectiveness of cardiovascular therapy. Aim: The study was conducted to identify the incidence and nature of DDIs and their associated risk factors among patients with cardiovascular disease (CVD). Materials and Method: A prospective interventional study was conducted between August 2018 and April 2019 in the Department of Cardiology of a tertiary care South Indian hospital. Patients admitted with CVD on a minimum of two medications were enrolled in the study and their prescriptions were evaluated for DDIs using Micromedex interaction checker version 2.8. Results: A total of 258 patients with CVD were enrolled in the study with a mean age of 60.11±12.07 years. On average, each patient was prescribed 11.35±3.30 medications. The incidence of potential DDIs (pDDIs) was 99.61%, with 7.59±4.10 as a mean number of pDDIs per prescription. The majority of the interactions were significant in severity (50.69%), pharmacodynamic (68.24%) in nature, of unspecified onset (69.97%), and with fair documentation status...
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Posted by admin on Aug 31, 2021 in |
Bio behavioural aspects, immunity, and disease course are interlinked in a majority of conditions, particularly cancer. Peripheral stressors or their products regulate immunity by various ways. When there is added insult in an already compromised immune state such as cancer, as is done here using lipopolysaccharide (LPS), the system is said to be ‘double-hit. Behavioural and brain tissue-associated biochemical changes on LPS challenge have been assayed in a Diethylnitrosamine (DEN)-treated Wistar rat model of hepatic cancer. Peripheral and central effects of LPS challenge have been screened at levels of neuro behaviours, liver enzymes and brain enzymes. While LPS challenge reduced exploratory and grooming behaviours across groups after 2 h, the effect disappeared on testing after 4 h, 6 h post LPS injection, rats demonstrated increased open arm time, reduced closed arm time and increased entries into both arms of the elevated plus-maze. DEN treatment induced similar results. LPS challenge in DEN-treated rats however, led to reduced open arm time, increased closed-arm time, and reduced entries into both arms. LPS...
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Posted by admin on Aug 31, 2021 in |
A Novel virus, coronavirus 2 (SARS-CoV-2), in 2019 from Wuhan, China which is globally expanded, causing the severe acute respiratory syndrome. Coronavirus is transmitted human to animal or human to human through airborne droplets. Coronavirus causes respiratory infections like pneumonia, cold, sneezing and coughing, while in animals, it causes diarrhoea and upper respiratory diseases. The virus outbreak has been declared a public health emergency of international concern by the World Health Organization (WHO), Since Covid -19 rapidly spread throughout the world from the Hunan seafood market at Wuhan, South China. The virus enters into the human cell through the membrane ACE-2 exopeptidase receptor. WHO and ECDC guided to stay away from a public place and close contact with contaminated individuals and pets. Coronavirus (2019-CoV), first and foremost, was isolated on 7th January 2020 at Wuhan market China. Here we have encapsulated the current clinical features data to guide budding Covid-19 about the Prevention, Diagnosis, Treatments and Prevention. Throughout the world, the disease has caused a range of degrees of...
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Posted by admin on Aug 31, 2021 in |
A RP-UFLC method was developed &validated for simultaneous estimation of Sitagliptin Phosphate and Metformin HCl in bulk and tablet dosage form. Separation was achieved by using Phenomenex Luna C18 (150 × 4.6mm, 5µm) column. The mobile phase comprises MeOH: Phosphate Buffer of pH 7 using Triethylamine in the ratio (60:40v/v). The flow rate was 0.6ml/min at temperature 300C. The instrument used for UFLC was Shimadzu autosampler separation model LC 20AD. Quantification was achieved with UV detection using a photodiode array detector at 258nm wavelength. The injection volume was 20µl. The retention times of Sitagliptin Phosphate & Metformin HCl were found to be 7.027 & 2.802, respectively. The linearity range of Sitagliptin Phosphate was 50-100µg/ml & Metformin HCl was 10-60µg/ml. The correlation coefficient was found to be within limits, i.e., 0.999. The % purity of Sitagliptin Phosphate & Metformin HCl was found to be 101.99% and 101.73%. LOD values were found to be 2.21437 for Sitagliptin Phosphate & 1.99084 for Metformin HCl and LOQ values were found to be 6.71024...
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Posted by admin on Aug 31, 2021 in |
A simple and precise stability-indicating reversed-phase (RP-HPLC) method was developed and validated for the determination of Apixaban active pharmaceutical ingredient. The chromatographic condition was carried out on Phenomenex Luna 5 µm C18 100 Å (150 × 4.6 mm) HPLC column with prepared buffer solution 1.0 ml of Orthophosphoric acid mixed with 0.2% of potassium dihydrogen phosphate solution, use as mobile phase (a), mobile phase (b) was acetonitrile. The mobile phase flow rate at 1 ml/min and the detection wavelength is 230 nm. Forced degradation study was covered acid hydrolysis, base hydrolysis, peroxide, photolytic (UV light) and thermal degradation was performed to prove the specificity of the proposed method and degradation was achieved. The developed method has been statistically validated according to ICH guidelines and found to be simple, precise, linear and accurate with the prescribed values. Thus the proposed RP-HPLC method was successfully applied for the substance in routine quality control analysis in active pharmaceutical...
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