Posted by admin on Aug 31, 2020 in |
At present, the whole world fronting a very challenging situation of the pandemic, severe acute respiratory syndrome due to coronavirus (COVID-19). Today, COVID-19 is spreading rapidly in every part of the world; these pandemic affected billions of people. This virus is found as a new human pathogen. However, currently, there is no hasty therapy available, which will provide fruitful results in this regard. As per previously reported data, human convalescent serum (plasma) will be a great choice for the rescue patients from COVID-19 infection, and it will be readily available if there are adequate numbers of people who have recovered and can donate immunoglobulin consisting serum. It includes the administration of antibodies against a given agent to an affected individual for the cause of treating an infectious disease due to the virus. In disparity, active vaccination involves the induction of an immune response that takes time to develop and varies depending on the vaccine recipient. The plasma therapy has a storied history going back and founds as well reported...
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Posted by admin on Aug 31, 2020 in |
The current pandemic of the novel coronavirus (SARS-CoV-2) termed as COVID-19 is the third recorded blowback of an animal coronavirus to a human being in the last twenty years. The pandemic is originated from China and currently impacted more than 205 countries worldwide (as on dated 2nd April 2020). This pandemic is not only affecting human life but also has questioned the worldwide status of healthcare facilities and resulted in a slowdown of the economy. But the fact is the SARS-CoV-2 virus, which causes Covid-19, has less virulence but a high infection rate than SARS-CoV-1, which caused an outbreak of SARS in 2003. The disease is mainly transmitted either through close contact with an infected person via respiratory droplets or by touching a contaminated surface. Treatment involves a combination of certain potent antiviral drugs. SARS-CoV-1 and SARS-CoV-2 share 80 to 90 percent genetic similarity; thus, the treatments which are effective against SARS-CoV-1 can be adjuvant therapeutic strategy against SARS-CoV-2. Some of the essential oils with certain active compounds show...
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Posted by admin on Aug 31, 2020 in |
A simple, sensitive, and rapid Liquid Chromatography-Mass Spectroscopy method was developed and validated for the quantification of Isoproterenol in human plasma using Dobutamine as an internal standard. The method utilized simple liquid-liquid extraction using a mixture of diethyl ether and dichloromethane for the sample preparation involved prior to LCMS analysis. The analytes were chromatographed on Prontosil ODS C18 Column with isocratic elution using methanol, Acetonitrile and Tri ethyl-amine in the ratio of 60:25:15 (v/v) at pH 6.3 as the mobile phase at a flow rate of 0.9 mL/min and the UV detector response of the column eluents were recorded at a wavelength of 248 nm. Quantification was performed in multiple-reaction-monitoring mode with the ion transitions m/z 212.19 → 135.21 for Isoproterenol, m/z 302.19 → 107.05 for Dobutamine. Good linearity was obtained in the range of 0.50–300 ng/mL (r2 = 0.999). The method was fully validated with accuracy, precision, matrix effects, recovery and stability. The results of the stability study confirm that the method was found to be stable. The...
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Posted by admin on Aug 31, 2020 in |
In this planed research work, aryl piperazine derivatives will be synthesized because aryl piperazine currently the most important constructive block in drug discovery with positive pharmacological evaluation. A series N- (4- (benzo[d]thiazol-2-yl) phenyl)-2-[4-(aryl-substituted) piperazines-1-yl]acetamide, N-(4-(benzo[d]oxazol-2-yl)phenyl)-2-[4-(arylsubstituted)piperazines-1-yl]acetamide and Synthesis of N-(4-(benzo[d]imidazol-2-yl) phenyl)- 2- (4- (arylsubstituted)piperazin-1-yl) acetamide will be synthesized with their characterization such as melting point determination, Thin-layer chromatography (TLC) and spectral analysis. After that, pharmacological evaluation, such as antibacterial activity, will be performed for synthesized compounds. All the synthesized compounds were reported for determination of zone of inhibition (mm), minimum inhibitory concentrations (MIC) were also calculated for effective derivatives, with an objective to offer some potent antimicrobial agents to human beings. Antimicrobial activity is determined based on their in-vitro activity in pure cultures. In-vitro susceptibility testing is done by two methods i.e., Turbidimetric/photometric/tube dilution method and agar diffusion/cup-plate/cylinder plate...
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Posted by admin on Aug 31, 2020 in |
SARS-CoV-2 or COVID-19 is one of the deadly pandemics faced by the world population, which has infected 7 million and claimed the lives of 0.4 million people. In spite of a few drugs available to control the pandemics, a formal vaccine is the least that the world expects under the current scenario. However, the release of a vaccine is expected to come at the cost of its own time. SARS-CoV-2 replicates in the host cells with the aids of the molecular machinery of a complex formed by three non-structural proteins (NSPs) viz., nsp12, nsp8, and nsp7. Recent studies reveal that among the three NSPs, nsp12 is vital for viral replication and is the target for drugs. Several studies have linked the viral infection to a weaker immune system, which is quite likely to be targeted by the virus. In search of such a natural compound that might increase the immunity and block the viral replication within the host, we selected C-Phycocyanin of Spirulina plantesis to study its anti-viral property...
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