Posted by admin on Aug 31, 2020 in |
This experimental study had approved by animal ethics of Central Drug Research Institute, Lucknow, and had undertaken to evaluate the antidyslipidemic and anti-oxidant activities of Nigella Sativa (N. Sativa), Hindi name Kalonji seeds extract in two models of hyperlipidemia. 1- triton and 2- cholesterol-rich high-fat diet (HFD) induced hyperlipidemia. N. Sativa and Gemfibrogil were macerated with 2% aqueous gum acacia, and the suspension had fed orally to rats at a dose of 500 mg/Kg (b.w.p.o.) respectively. Serum lipids were found to be lowered by N. Sativa in triton induced hyperlipidemia. On the other hand chronic feeding of N. Sativa extracts to rats in cholesterol-rich high fat diet-induced hyperlipidemia for 30 days caused lowering in lipid and apoprotein levels of β-lipoproteins followed by an increase in lipid and apoprotein levels of α lipoproteins. The results of the present study also demonstrate that N. Sativa seeds extract repaired hepatic lipid synthesis, increased fecal bile acid excretion, and increased plasma LCAT activity in rats. Furthermore, N. Sativa seeds extract (100 to 400...
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Posted by admin on Aug 31, 2020 in |
In the present study, an eco-friendly, cost-efficient, rapid and simple approach was applied to the synthesis of silver nanoparticles by using a methanolic extract of bulb of Zephyranthes citrina. The metal salt was reduced to ions by metabolites present in bulb extracts. The plant extracts acts both as a reducing agent as well as a capping agent. The synthesized silver nanoparticles were characterized by various spectral techniques like UV-Visible, FT-IR, SEM, EDAX and XRD analysis. Biosynthesis of silver nanoparticles was confirmed by viewing the colour change of the extract from brown to blackish brown. The functional group which is responsible for the reduction of silver ions in the plant extract was characterized by FT-IR. UV-Visible spectrophotometer showed an absorbance peak at 277 nm for silver nanoparticles. The SEM image depicts the nanosized particles with morphological shape. The elemental composition was determined by EDAX analysis. The XRD results confirmed the crystal structure of the nanoparticles. The spectral data confirm the formation of nanoparticles with its size, morphological structure and chemical...
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Posted by admin on Aug 31, 2020 in |
The present paper reveals the socio-economic benefits for the peoples of the Uttarakhand state. The demand for medicinal and aromatic plants of Uttarakhand is quite inflated, and many of these plants grow only in the Himalayan states. The State has diverse agro-geo climate conditions, which is most suitable for Medicinal and aromatic plant cultivation. Uttarakhand is blessed with a variety of soils and agro-climatic conditions, ranging from Sub-Tropical to Alpine, which is a mega biodiversity hotspot for a wide range of wild and cultivated Medicinal and aromatic plants. Due to the low point of agricultural production, lack of industrial development, poverty, and unemployment, peoples migrate to exploit biodiversity to improve their socio-economy. There is a socio-economic pressure to migrate on the plain districts for better livelihood...
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Posted by admin on Aug 31, 2020 in |
The L-Glutaminase enzyme is a therapeutic agent that can be employed for the treatment of human cancer, specifically lymphocytic leukemia. Microbes are exploited commercially to produce L-Glutaminase on a large scale. The L-Glutaminase is an economic anti-cancer drug and can be easily administered into patients. The L-Glutaminase is also employed in food industries in the processing of fermented foods. In the present paper, the L-Glutaminase enzyme structure was developed and validated using web-based computational tools. The sequence (FASTA format) of the L-Glutaminase enzyme was obtained from UniProt Knowledgebase. The FASTA format sequence of the L-Glutaminase enzyme was used as input data in the SWISS MODEL workspace to develop the structural model of L-Glutaminase by automated mode. The quality of the modeled structure of the L-Glutaminase enzyme was checked in PROCHECK server and SPDBV (Swiss PDB Viewer) and validated. Further, L-Glutaminase activity can be enhanced using advanced bioinformatics tools. Such L-Glutaminase with improved activity can be produced by...
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Posted by admin on Aug 31, 2020 in |
Notable progress has been made in methods that encourage the use of PCR as a rapid and accurate tool in quality evaluation of pharmaceuticals. In this study, monoplex and multiplex PCR based assays were developed and compared with standard conventional methods for rapid detection of three specified topical indicator pathogens, Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans, in nonsterile pharmaceutical preparations. The detection limit of monoplex PCR assays for the microbial targets was achieved at 100 fg purified DNA and 10 CFU/ml for Pseudomonas aeruginosa, and 1fg purified DNA and 10 CFU/ml for each of Staphylococcus aureus and Candida albicans. No change in the detection limit for CFU/ml of the three tested indicator pathogens was obtained upon using mPCR assays. The results of applying both conventional and PCR detection methods for different cream and lotion preparations revealed a 100% correlation between both methods. The PCR based detection method can be completed in 8 h versus 5-6 days in case of conventional methods, but the former can’t differentiate between viable...
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