Posted by admin on Aug 31, 2020 in |
Notable progress has been made in methods that encourage the use of PCR as a rapid and accurate tool in quality evaluation of pharmaceuticals. In this study, monoplex and multiplex PCR based assays were developed and compared with standard conventional methods for rapid detection of three specified topical indicator pathogens, Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans, in nonsterile pharmaceutical preparations. The detection limit of monoplex PCR assays for the microbial targets was achieved at 100 fg purified DNA and 10 CFU/ml for Pseudomonas aeruginosa, and 1fg purified DNA and 10 CFU/ml for each of Staphylococcus aureus and Candida albicans. No change in the detection limit for CFU/ml of the three tested indicator pathogens was obtained upon using mPCR assays. The results of applying both conventional and PCR detection methods for different cream and lotion preparations revealed a 100% correlation between both methods. The PCR based detection method can be completed in 8 h versus 5-6 days in case of conventional methods, but the former can’t differentiate between viable...
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Posted by admin on Aug 31, 2020 in |
Background: Self-Nanoemulsifying Drug Delivery System (SNEDDS) has been employed extensively by the formulation of scientists to tackle the low solubility issues of various drugs and lift the bioavailability profile. But the potential of SNEDDS is not limited to augment the dissolution profile only. Objective: The objective of the study was to develop, optimization, and characterization of SNEDDS of anti-lipase inhibitors. Method: Orlistat was added in an accurately weighed amount of oil into a screw-capped glass vial and heated in a water bath at 40 ºC. The surfactant and co-surfactant were added to the oily mixture using positive displacement pipette and stirred with a magnetic bar. The formulation was further sonicated for 15 min and stored at room temperature until its use in subsequent studies. A 23 full factorial design was selected because an experiment may be designed to focus attention on a single independent variable or factor. Results: The drug loading efficiency for all Orlistat SNEDDS formulae was found in the range of 92.37% ± 0.75% (PF1) to 99.09%...
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Posted by admin on Aug 31, 2020 in |
Innovation in technology is required to change the world. Bioelectronic medicine is the consolidation of molecular medicine, neuroscience, engineering, and computing to develop a device to diagnose and treat diseases. The mechanisms of Bioelectronic medicine for neural control of a biological process that underlie disease and the development of devices to modulate these specific neural circuits as therapy using electrons instead of drugs. Bioelectronic medicine has emerged at a convergent epicenter in health care, technology, and science. Bioelectronic medicine is a new way to treat disease. Today patients are treated by either drug, which can lead to a side effect or drive up costs, which can mask pain signals but they usually can’t mask the central cause of disease. With the rapid rise in technology for the precision detection & modulation of electrical signaling patterns in the nervous system is a new class of treatment known as bioelectronic medicines. Specifically, the peripheral nervous system will be at the center of this advance, as the functions it controls in chronic...
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Posted by admin on Aug 31, 2020 in |
Anti-inflammatory activity and the toxicity of Bohadschia vitiensis water extract were investigated by in-vitro models to evaluate the consumption of the extract by the local community to reduce arthritic-related pain. Specimens of B. vitiensis were collected from Mannar, Sri Lanka, and the water extract (WE) was prepared by removing visceral organs and incubating diced samples in distilled water, followed by freeze-drying. The WE was used to test acute toxicity, based on Danio rerio embryotoxicity assay and to evaluate anti-inflammatory activity based on in-vitro models; egg albumin denaturation, erythrocyte membrane stability, Nitric Oxide (NO) scavenging and Hydrogen Peroxide (HP) scavenging activities. The IC50 was calculated for each assay and compared with a standard reference drug. The qualitative zoo-chemical analysis was carried out while the presence of major nutrients was evaluated. According to the results, WE had a LC50 of 151.59 µg/ml for Danio rerio embryotoxicity assay. The anti-inflammatory activity against egg albumin denaturation (IC50 of 277.51 µg/ml) was reported while a maximum percentage inhibition of 32.09% was reported at 250...
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Posted by admin on Aug 31, 2020 in |
Salvia hispanica L. Well known as chia is gaining popularity day by day due to its nutritional value. This plant is native to Mexico, belonging to family Labiatae / Laminacea, and it was used as a superfood from ancient times. Chia is valued more due to its oil content as it consists of omega -3-alpha linolenic acid in higher amount along with various types of other nutrients, e.g., proteins, dietary fibers, antioxidants, etc. which is very beneficial to keep a person healthy and also it helps in prevention of many diseases like CVS, diabetes, obesity cancer and also it gives strength to our immune system. In the past few decades, we have noticed a certain growth in the market value of chia, and it is increasing day by day, not in a particular area or region but around the whole globe. In this paper, we have discussed all the important characteristics of chia (from its morphological characters to its pharmacological properties along with nutritional values and its today’s marketing...
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