Posted by admin on Aug 31, 2017 in |
Molecular docking studies of 102 newly designed N-substituted imidazoles was performed in the non nucleoside inhibitory binding pocket of reverse transcriptase enzyme of wild type as well as resistant strains of HIV-1 virus with PDB ID 1RT2, 1JLB, 3BGR and 1FK9 respectively by using Glide 5.0 to carry out a binding mode analysis of the designed imidazoles. Results generated from this study indicate that most of the compounds dock into the active site of different enzymes such as 1RT2, 1JLB, 3BGR and 1FK9 showing excellent Docking scores comparable to the standard TNK 651. Compounds SRS 21, SRS 34, SRS 45, SRS 46, SRS 73, SRS 76 and SRS 77 exhibited the highest docking scores and were found to be most effective as compared to standard TNK...
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Posted by admin on Aug 31, 2017 in |
Objectives: Tuberculosis (TB) remains one of the most substantial infectious causes of death in the world. Studies focusing on the quality of life (QOL) among TB patients are limited and the effect of counselling on patients QOL has not been well-studied. The aim of this study was to assess the effect of patient counselling on QOL among patients with Tuberculosis. Methods: A quasi-experimental study was conducted over 6 months at a tertiary care hospital after permission from the Institutional Ethics Committee. Tuberculosis patients between 18 and 65 years and were taking anti-tubercular therapy were included. Their physical component scoring (PCS) and mental component scoring (MCS) were assessed using SF-12®questionnaire. Counselling was provided to all the patients and PCS and MCS were assessed again after 14 days. Results: A total of 109 patients were taken for the study. There was an improvement in physical and mental status of TB patients. Conclusion: Improved physical and mental status implies the positive impact of patient...
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Posted by admin on Aug 31, 2017 in |
Recently, biological synthesis of nanoparticles (NPs) has attracted attention because of the need to develop new cost-effective and efficient synthesis protocol. In this paper, we account for the use of cell-free extract of Spirulina platensis in the biosynthesis of copper oxide nanoparticles (CuO NPs) of dimensions 30 – 40 nm. The biosynthesised CuO NPs were confirmed visually by appearance of dark brown colour formation in mixture and surface plasmon resonance band observed at 259 nm by using UV-Visible Spectroscopy. The size and morphology of the nanoparticles were confirmed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) analysis. Furthermore, these NPs were found to exhibit high antibacterial activity against pathogenic gram-negative i.e. Escherichia coli – MTCC-9721, Proteus vulgaris – MTCC-7299, Klebsiella pneumonia – MTCC-9751 and gram-positive i.e. Staphylococcus aureus – MTCC-9542, S. epidermidid -MTCC- 2639, Bacillus cereus – MTCC-9017 bacteria. The CuO NPs had shown maximum zone of inhibition (ZOI) i.e. 28.0 ± 0.41 mm in P....
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Posted by admin on Aug 31, 2017 in |
Marine algae are one of the largest sources for biogenic compounds. They produce a wide variety of chemically active metabolites. They are found to possess significant antioxidant, and phytochemical activities. Macroalgae provides a great variety of metabolites and natural bioactive compounds with antimicrobial activity, such as polysaccharides, polyunsaturated fatty acids, phlorotannins and other phenolic compounds, and carotenoids. Several species of brown algae have been investigated for their bioactive natural products. The aim of the present study was to investigate the antioxidant, antibacterial activity and GC-MS analysis of active compounds present in the methanol extract of the brown algae, Padina tetrastromatica. For antioxidant activity, DPPH radical assay, ABTS radical cation scavenging assay, phosphomolybdenum reduction assay and Fe3+ reducing power assay were carried out. The maximum DPPH radical scavenging activity was 54.78%. The extract also exhibited significant antibacterial activity against the clinical pathogens, Vibrio cholera, Shigella flexneri and Pseudomonas aeruginosa. The phytochemical analysis showed the presence of alkaloids, terpenoids, steroids, flavonoids and phenolic compounds. GC-MS analysis revealed the presence of fatty...
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Posted by admin on Aug 31, 2017 in |
The trigeminal ganglion consists of pseudounipolar neurons surrounded by satellite glial cells and processes innervating craniofacial region. The trigeminal ganglion consists of neurons which are of first order pseudounipolar carrying sensory pathway from the receptors from face, mouth, scalp and duramater. The gap junctions are trans-membrane proteins formed between the cell membranes of adjacent cells and calcitonin gene related peptide are neuro-peptides secreted by sensory neurons. In present study the immune histochemical localization for connexin 36 gap junctions and CGRP was done in the trigeminal ganglion of male Wistar rats. The result showed connexin 36 was present between the satellite glial cells, between satellite glial cell and neuron and in the schwann cells. CGRP was localized densely in the cytoplasm of small neurons. The excited neuron can influence the surrounding satellite glial cells and neurons through gap junctions and by paracrine actions altering its environment leading to pathological role in inducing painful conditions like...
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