Posted by admin on Aug 31, 2016 in |
Overweight and obesity rates have been increasing sharply over recent decades in all industrialized countries, as well as in many lower income countries. According to the WHO World health statistics report 2012, globally one in six adults is obese and nearly 2.8 million individuals die each year due to overweight or obesity 1. Obesity is a close marker of important aspects of individual life styles, such as diet and physical activity, and is also an important risk factor for major chronic diseases, such as diabetes, heart disease, stroke and certain type of cancer. Obesity is also associated with negative labour market outcomes, in terms of both wages and employment, particularly for women 2. Several studies show that education has a positive impact on health and well-being 3.This is a prospective observational study, conducted with 750 patients in Kerala. The study conducted strictly observed the prevalence of obesity in a particular area of kerala, risk factors, concomitant disorders and awareness of patients about obesity and related illness in detailed and...
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Posted by admin on Aug 31, 2016 in |
Background: Urinary tract infection is a common medical problem for women throughout their lifetime. Recurrence after urinary tract infection (rUTI) is common in adult women. The majority of recurrences are believed to be reinfection from extra urinary sources such as rectum or vagina. The treatment of recurrent urinary tract infection also inflicts an economic burden on the patient’s life. Hence, we must use an effective antibiotic that prevents the recurrence of UTI and improve the functional capacity and quality of life in women. This study aims to study the effectiveness of different antibiotics in increasing the functional capacity and reducing the economic burden in UTI women. Methodology: This is a prospective, observational study, conducted in a total of 50 patients at General Medicine Department of a tertiary care hospital in Coimbatore for a period of six months. The patient demographic details were collected from the medical records or direct patient interview. Urine analysis, urine culture and antibiotic sensitivity reports were also collected and entered in the data collection form....
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Posted by admin on Aug 31, 2016 in |
Chloramphenicol was introduced as the effective antibiotic in the treatment of typhoid fever. Resistance started to develop within two years of its introduction. The emergence of antibiotic resistant strains of bacteria is closely linked to the irrational use of antibiotics. The sensitivity/resistance pattern of Salmonella spp. had been varying with time and geographical locations. Changing trends in antibiotic resistance patterns especially of chloramphenicol have been recorded in different parts of India. So the study was done to evaluate the minimum inhibitory concentration of chloramphenicol. Two hundred clinically suspected cases of enteric fever were included in the studies during the period of one year and nine months. Positive blood culture for Salmonella spp. was 8%. Isolates of Salmonella Typhi were 100% sensitive to cotrimoxazole, chloramphenicol, and cefotaxime. 12 (75%) were sensitive to ampicillin, 7 (43.7%) to azithromycin, 14 (87.50%) to ceftriaxone and 10 (62.5%) were sensitive to cefixime. The isolates show 100% resistance to ciprofloxacin and nalidixic acid. The minimum inhibitory concentration (MIC) for all the chloramphenicol sensitive isolates ranged...
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Posted by admin on Aug 31, 2016 in |
The present paper describes a new, simple, accurate, environmentally friendly, economic spectrophotometric method for simultaneous estimation of Diclofenac salt and Eperisone HCl in bulk and Capsule Dosage Form. Acetate buffer pH5.5 was used as solvent. The wavelength selected for analysis is 276nm & 261.20nm for estimation of Diclofenac Salt and Eperisone HCl respectively. Beers law was obeyed in the range of 10-50µg/ml and 5-25µg/ml with correlation coefficient of0.999 and 0.998 respectively. Detection limit and quantification limit were found to be 0.6031g/ml and 1.87g/ml for Diclofenac sodium and 0.208g/ml and 0.6302g/ml for Eperisone hydrochloride respectively. The developed method was found to be simple, accurate, precise and reproducible. Assay results were found to be 99.20% and 101.13% for Eperisone hydrochloride and Diclofenac sodium respectively. The value of relative standard deviation and percentage recovery was found to be satisfactory. The obtained results proved the method can be used for routine analysis of Diclofenac sodium and Eperisone Hydrochloride in capsule dosage form. The method where tested and validated as per ICH...
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Posted by admin on Aug 31, 2016 in |
Docking and site directed mutagenesis approach was used to explore mode of binding and inhibition for human acetylcholinesterase (hAChE) and organophosphates (OPs). More than 200 OP molecules were investigated using Glide docking module of Schrodinger suit as co-crystal structure between two are not available in Protein Data Bank. In initial screening Trp86 was found to be involved in maximum Pi-Cation interaction on anionic subsite of hAChE other than Ser203 (Catalytic site). With extra precision glide docking Phoxim Ethyl Phosphonate (PEP) tops among 200 OPs based on glide docking score and interacted with Trp86, Gly121 and Ser203 whereas MM-GBSA score shows less binding affinity than heptenophos and dichlorovos. Trp86 preferred Pi interaction with ring bearing OPs and hydrophobic interactions with smaller OPs without ring bearing structures. Site directed mutagenesis at Trp86 (Trp86 to Ala86) shown the deterioration of the binding site in terms of size reduction, loss of electrostatic and geometric stabilization in binding cavity and significant reduction in binding of OPs in preferred orientation. Dock score of both wild...
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