Posted by admin on Dec 1, 2010 in |
This paper describes the analytical method suitable for validation of Candesartan Cilexetil (CDN) by reversed Phase liquid chromatography (RP-LC) method. The method utilized RP-LC (Shimadzu LC-10AT with UV detector) model and Hypersil ODS C-18 (250 x 4.6 mm, packed with 5 micron) column was used for the separation. The mobile phase was consists of acetonitrile:0.05 M KH2PO4 buffer in the ratio of 65:35 at flow rate of 1.5 ml/ min. Validation experiments were performed to demonstrate System suitability, Specificity, Precision, Linearity, Accuracy study. The method was linear over the concentration range of 0.5-400 mg/ml. The method showed good recoveries (99.54- 100.41%) and the recovery studies were carried out by adding different amounts (80%, 100% & 120%) of bulk samples of Candesartan cilexetil along with internal standard were 100.04%, 99.98% & 99.64% respectively. The proposed method is Precise, Accurate, Reproducible and rapid for the determination of Candesartan cilexetil in bulk and their pharmaceutical dosage...
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Posted by admin on Dec 1, 2010 in |
The present investigation deals with the development of a new, simple, specific, sensitive, rapid and economical procedure for simultaneous estimation of Carvedilol in its dosage form. The method is based on the ultraviolet absorbance maxima of the above drug at 285 nm. The drug obeyed Beer’s law in the concentration range of 4-36 μg/ml with molar absorptivity of 12.6x 103 l/mol.cm in methanol. The proposed methods were successfully applied for the simultaneous determination of drug in commercial tablet preparations. The results of the analysis have been validated statistically and by recovery...
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Posted by admin on Dec 1, 2010 in |
Calotropis procera (Asclepiadaceae) is an erect, perennial shrub luxuriantly thriving in wastelands. It is commonly distributed in most parts of India and also distributed in Persia, tropical Africa and other tropical countries of the World. The aqueous extract of some parts have laxative property and beneficial in skin diseases, ulcers, enlargement of the spleen, abdominal glands, liver diseases, promotes digestive power and also useful in colics. The milky exudate is useful in skin diseases, tumour and ascitis. It is a very good purgative. Here an attempt has been taken to evaluate the antioxidant activity, CNS depressant activity and also antimicrobial activity of methanolic extract of fresh flowers of C. procera. In result, the methanolic flower extract showed a significant activity against DPPH free radicals and the IC50 value is 64.87µg/ml. On the other hand, CNS depressant activity of the extract was also observed by using In Vivo model. In antimicrobial activity five different fungal species were inhibited by this extract and from the above study the flowers extract showed...
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Posted by admin on Dec 1, 2010 in |
Garcinia indica is well spread in tropical rain forest of Western Ghats of India. Extract of dried fruit rind of kokum was prepared using methanol. Extract was evaluated for antioxidant and antihyperlipidemic activity. Garcinia was tested for antihyperlipidemic activity using cholesterol induced hyperlipidemic model in Albino Wistar rats. It exhibited significant reduction in total cholesterol, triglycerides, LDL-C, VLDL-C levels and increase in level of HDL-C when compared to Lovastatin. Artherogenic index and LDL-C: HDL-C ratio was also reduced to significant extent. Significant antioxidant and antihyperlipidemic activity may be due to the presence of polyphenols like Garcinol and hydroxycitricacid in...
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Posted by admin on Dec 1, 2010 in |
In the present study, Solanum melongena Linn. (Brinjal; Solanaceae), was evaluated for its antiepileptic activity against experimental seizures. The roots ethanolic extract of Solanum melongena protected mice against tonic convulsion induced by maximal electroshock, Pentylenetetrazole and Picrotoxin. It was found that ethanolic extract up to a dose of 300 mg/kg p.o. decreased the duration of tonic extensor phase and delayed the onset of time of jerk against maximal electroshock induced seizure and pentylenetetrazole induced seizure. In Picrotoxin induced seizure, the extract at a dose of 200 mg/kg significantly increased the onset of clonic- tonic convulsion. These findings suggest that extract of the roots of Solanum melongena posses anticonvulsant effect and reduce mortality. The further studies to determine the mechanism(s) and compound(s) involved in the anticonvulsant...
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