Posted by admin on Mar 31, 2020 in |
In-vitro investigations toward DNA damage reported that the biological effect of genotoxic exposures could influence the life cycles of different human cells. Sister chromatid exchange (SCE) assay is broadly used, sensitive cytogenetic biomarker of exposure to genotoxic agents. Naturally available products may play a significant role against the cytogenetic damage but available research data is not consistent. The purpose of this study was to evaluate the anti-genotoxic potential of beet root extract (BRE) against hair dye induced genotoxicity in cultured human peripheral blood lymphocytes (PBLs) using SCE assay. For this, PBLs were treated with different doses of hair dye ingredients along with BRE. Significant (p<0.05) rise in SCE frequency indicates genotoxicity of hair dye ingredients (p-Phenylenediamine-200 µg/ml, resorcinol-200 µg/ml, and hydrogen peroxide-6.168 µg/ml). Treatment of BRE along with hair dye showed significant (p<0.05) reduction in SCE frequency in a dose-dependent manner as compared to hair dye treated...
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Posted by admin on Mar 31, 2020 in |
Objective: Mycoplasma hominisis a gram-negative bacteria belonging to class Mollicutes that is commonly present in men and women of reproductive age. At present, there is no effective prophylaxis for Mycoplasma hominis, as it has gained resistance for the drugs hence an in-silico approach was undertaken to find out the peptide-based vaccine for the pathogen. Methods: In women, it affects the genital tract and involved in causing pelvic inflammatory disease, ectopic pregnancy, early delivery, miscarriage and prolonged infection may lead to infertility. M. hominis can also be transmitted from mother during childbirth and cause fever and infection in the new-born baby. All the 529 protein sequences of Mycoplasma hominis were taken in FASTA format from the UniProt proteome database. Antigenicity study was done for all the proteins of the pathogen using VaxiJen v2.0 and the proteins with high antigenicity scores were taken for further analysis like structural and functional analysis using InterPro, molecular docking using Autodock and molecular simulation study was carried out using Charmm. Results: The study identifies 50S ribosomal protein L28,...
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Posted by admin on Mar 31, 2020 in |
Lymecycline is a tetracycline broad-spectrum antibiotic. Present work describes a simple, accurate, precise, economical and reproducible spectrophotometric method in ultraviolet region has been developed and validated for the assay of Lymecycline in bulk and in Pharmaceutical formulations in diluent. Lymecycline is a tetracycline broad-spectrum antibiotic. Lymecycline like other tetracyclines is used to treat a range of infections Lymecycline exhibits absorption maxima at 270 nm in diluent. Beer’s law was found to be obeyed in the concentration range of 7.5-22.5 μg/ml. The optimum concentration of the Lymecycline was found to be 15 µg/ml. This concentration of Lymecycline was shown good absorbance valves at respective wavelengths was found to be 0.490. Linearity studies were carried out and the range was found to be 7.5-22.5 µg/ml for Lymecycline in diluent. The regression coefficient value of Lymecycline was found to be 0.999 which was not less than 0.995. The method is accurate, precise and economical. In this proposed method, there was no interference from common pharmaceutical excipients. The results of the analysis were...
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Posted by admin on Mar 31, 2020 in |
A number of strategies to deliver antifungal using nanocarriers are developed to facilitate drug targeting infected cells that result in improved antifungal activity. Objective: Ciclopirox olamine has a broad spectrum of action against dermatophytes, yeasts, filamentous fungi and bacteria. It has a biological half-life of 1.7 h and bioavailability of < 5% with prolonged use. A remarkable feature of the drug is that no single case of fungal resistance has been reported so far. Therefore, ciclopirox olamine loaded niosomes entrapped in suitable gel delivery systems may successfully deliver the drug for prolonged time in treatment of fungal infection. Material and Method: A Ciclopirox Olamine loaded niosomes were developed using various surfactants along with cholesterol in different proportions by ether injection and film hydration method. The prepared niosomes were evaluated for various characteristics such as shape and size, entrapment efficiency, in-vitro drug release studies. Result and Discussion: Among all, formulation CNFS61 showed optimal drug release with maximum entrapment efficiency having smaller vesicular size. So, formulation CNFS61 was considered as promising...
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Posted by admin on Mar 31, 2020 in |
Remarkable pharmacological effects exhibited by Guggulsterone (GS) establish its potential as an effective anticancer agent. GS is widely known for its anti-inflammatory and hypolipidemic activities. At the molecular level, GS is known to modulate the activity of various nuclear receptors, growth factors and transcription factors. However, the complete sequence of the underlying anticancer mechanism of GS is not completely understood. Prostate cancer (PCa) initiation is marked with inflammatory events and disease advances towards castration-resistant prostate cancer (CRPCa) through constitutively activated androgen receptor and hyperactivated survival signaling such as NF-kB; therefore PCa cell lines offer a good model system to study the anticancer activities of GS. Considering the fact that GS is both anti-inflammatory and androgen receptor (AR) antagonist, it can be widely employed to achieve two objectives simultaneously – chemoprevention as well as an anti-cancer agent. Evidence from preclinical trials and prescribed usage in Ayurvedic medicine has promoted the use of guggul as a health supplement. However, the use of GS is not completely free of side effects and...
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