Posted by admin on Mar 31, 2021 in |
Objective: To assess the efficacy of Ma-ul-Sha’eer (Barley water) as a dietary intervention in Intermediate Hyperglycemic subjects. Methods: A hospital-based prospective, pre and post interventional studies, conducted on 40 diagnosed cases of Intermediate Hyperglycemia (Pre-diabetes) as per WHO and ADA criteria at OPD of Majeedia Unani Hospital and A & U Tibbia College and Hospital, Delhi. Results and Discussion: The efficacy of Ma-ul-Sha’eer (Barley water) was mainly observed on blood sugar fasting and HbA1c. The mean value of blood sugar fasting before the intervention was 113.6±6.7, and after the intervention was 103.0±5.4. Paired ‘t’ test was applied to the observations recorded before the intervention and after the intervention. It was observed that t=14.5 (p <0.0001) and were statistically highly significant. The most probable mechanism encountered in reducing blood sugar fasting may be the presence of β-glucan in Ma-ul-Sha’eer. The mean value of HbA1c before the intervention was 6.0±0.21 and after the intervention was 5.4±0.27, Paired‘t’ test was applied to the observations recorded before the intervention and after the intervention....
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Posted by admin on Mar 31, 2021 in |
Medicinal plants are one of the most important resources for developing new lead therapeutic compounds used in various traditional medicines worldwide for thousands of years due to the presence of natural bioactive components, which help in preventing various human diseases. Piper betle Linn belongs to the Piperaceae family, commonly known as Betel and Paan. Betel leaves possess several bioactivities, and these are used in traditional medicinal systems. Syzygium aromaticum L. (Cloves) is a tree belonging to the Myrtaceae family. Closed flowering buds are used as a spice in food preparations all over the world. Eugenol is an active compound of both plants (Piper betle and Syzygium aromaticum). Hence, we attempted to study the effect of the methanolic extracts of Piper betle and Syzygium aromaticum, along with their active component, eugenol, using an in-vitro approach. The in-vitro cytotoxicity was assessed against lung adenocarcinoma cell lines (A549) using the MTT and SRB assays, and the type of cell death was characterized by differential staining. The results showed that the plant extracts,...
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Posted by admin on Mar 31, 2021 in |
A simple, accurate, and economical, least time-consuming method has been developed for Losartan potassium (LP) by using UV spectrophotometer. This method was developed using phosphate buffer solution pH 7.4 (PBS) for quantifying the amount of LP released from the colon-specific formulations. The wavelength maximum (λmax) of LP in phosphate buffer pH 7.4 was found to be 228.5 nm. The developed UV spectroscopic method exhibited linearity in the range of 2-10 μg/mL with a correlation coefficient (R2) value of 0.999 and was validated with respect to linearity, specificity, accuracy (recovery), and precision. These parameters were determined according to International Conference on Harmonization (ICH) guidelines. Results of the analysis were validated statistically and by recovery studies, which proved the suitability of the developed method for the routine estimation of LP in colon-specific control release formulation. Hence, the reported method for the estimation of LP from the colon-specific formulations was simple, accurate, and least...
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Posted by admin on Mar 31, 2021 in |
Poor bioavailability by the oral route is noticeable with the majority of new active pharmaceutical ingredients due to its dissolution rate-limited absorption. Gliclazide is an oral anti-hyperglycemic drug, though it is a class II drug, results in poor oral bioavailability. The present investigation was undertaken to prepare polymeric nanosponges of an oral anti-hyperglycemic drug Gliclazide to achieve improved solubility. Nanosponges using ethyl cellulose as a polymer and glutarldehyde as a cross-linker were prepared successfully by the emulsion solvent diffusion method. Drug polymer compatibility study was performed by FTIR and DSC. To obtain optimized batch, 32 factorial designs were performed. Optimized batch exhibited particle size 398. 78 nm, % drug content 90.504 ± 0.296, % entrapment efficiency 81.25 ± 0.266, % drug release 97.725 ± 0.186. An SEM and TEM image of optimized batch shows the spongy and spherical nature of formulations were converted into tablets to achieve immediate release drug delivery for oral route. These tablets were prepared using crospovidone and A SEM and TEM image of the optimized...
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Posted by admin on Mar 31, 2021 in |
The present study shows the cytotoxic effect of Dendrophthoe falcata (L. f) and Tridax procumbens Linn. extracts against HaCat (Skin cancer) cell line by MTT and SRB assay method. The phytochemical screening of extracts dichloromethane: methanol (DM), methanol: water (MW), and ethanol: water (EW) revealed the presence of secondary metabolites such as alkaloids, flavonoids, saponins, tannins, sterols, and triterpenes. % Cell inhibition by MTT assay of DFDM extract shows 90.00 ± 0.09% and TPDM 91.29 ± 0.02% at 80 µg/ml. IC50 values of DFDM extract <20 µg/ml and TPDM extract 20 µg/ml compare to IC50 values of standard 5-fluorouracil <10 µg/ml by MTT assay. SRB assay method DFDM extracts shows 86.96 ± 0.07% and TPDM shows 89.14 ± 0.27 with IC50 values < 20 µg/ml compare standard 5-fluorouracil <10µg/ml. HPTLC fingerprint analysis of active DFDM extract showed 11 peaks at 366 nm Rf values 0.06-0.87 & TPDM shows showed 12 peaks Rf values 0.33- 0.83 at 366 nm. Active extracts were analyzed agilent 7890A GC coupled with triple quadrupole...
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