Posted by admin on Mar 1, 2011 in |
Tinospora cordifolia, belonging to the Menispermaceae family, has been investigated for isolation of its secondary metabolites and evaluation of biological activities with special emphasis to the antimicrobial screening and cytotoxic study. The crude methanolic extract of Tinospora cordifolia along with its Pet ether, carbon tetrachloride, and chloroform soluble fractions were subjected to microbiological investigation by the disk diffusion method. The CCl4 soluble fraction was found to have mild to moderate anti-microbial action. In the brine shrimp lethality bioassay, the Pet ether, CCl4, chloroform and aqueous soluble fractions of crude methanolic extract were found to show LC50 1.918 mg/ml, 0.402mg/ml, 0.691 mg/ml and 2.246 mg/ml respectively. This indicated that the carbon tetrachloride and chloroform fractions were highly cytotoxic....
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Posted by admin on Feb 1, 2011 in |
Some novel benzimidazole derivatives were synthesized with ibuprofen and evaluated for its activity. This compound showed notable antibacterial activity against gram-positive organisms and antifungal activity against when compared with respective...
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Posted by admin on Dec 1, 2010 in |
The aim of present study was to investigate the phytochemical screening and to compare the antimicrobial activity of oils of Clove bud and Cardamom. Clove bud was successively extracted by steam distillation and isolated with Dichloromethane. The phytochemical analysis revealed the presence of alkaloids, glycoside, steroids, carbohydrates, terpenoids, tannins and phenolic compound. The dichloromethane extract was chromatographed over silica Gel (60-120) and eluted with pure toluene, toluene: Dichloromethane (9:1), toluene: Dichloromethane (8:2), toluene: Dichloromethane (7:3), fraction were monitored by T.L.C. similar fractions were combined and concentrated .eleven fractions were obtained and were labelled as f1, f2, f3 to f11. Cardamom fruit was successively extracted with petroleum ether. The phytochemical analysis revealed the presence of alkaloids, glycoside, steroids, protein, carbohydrates, terpenoids, tannins and phenolic compound. The Petroleum ether extract was chromatographed over silica Gel (60-120) and eluted with pure Benzene, Benzene: chloroform (9:1), Benzene: chloroform (8:2), Benzene: chloroform (7:3), Benzene: chloroform (6:4), Benzene: chloroform (5:5), Benzene: chloroform (4:6), and with pure chloroform. Fractions were monitored by T.L.C. similar fractions were...
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Posted by admin on Nov 1, 2010 in |
The aim of this study was to evaluate antimicrobial activity of the leaves of the Bauhinia racemosa L. In the current study, the aqueous and methanol extract of leaves of B. racemosa L. was tested against standard bacterial and fungal cultures. In vitro antimicrobial test was performed by agar well diffusion method on Mueller hinton agar and Sabouraud dextrose agar for bacterial and fungal cultures respectively. Minimum inhibitory concentration test was performed by modified agar well diffusion method. Methanol extract showed significantly higher inhibitory effect compared to aqueous extract on tested organisms. The methanol extract showed a broad spectrum of antimicrobial activity as it inhibited Gram negative bacteria (Escherichia coli, Micrococcus luteus, and Pseudomonas aeruginosa), Gram positive bacteria (Bacillus subtilis) and fungi (Candida albicans and Aspergillus niger). Both extracts showed maximum relative percentage inhibition against A. niger. MIC values for methanol extract varied from 1.5-25 mg/ml. Phytochemical analysis of crude methanol and aqueous extract suggested the presence of protein, oil and fats, phenolic compounds, flavonoids, saponins tannins and carbohydrates...
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Posted by admin on Oct 1, 2010 in |
Sphaeranthus indicus Linn (Asteraceae) is a common annual spreading herb found in rice field throughout India. Six crude extracts were prepared from the whole plant Sphaeranthus indicus using different solvents by cold maceration process. The extracts were subjected to screening to detect potential antimicrobial activity against S. aureus, B. substillus, P. Gentamycin and Nystatin as standard by cup plate agar diffusion method. The aim of our present study was to find out the antimicrobial activity of the different extracts of entire part including flower heads of Sphaeranthus indicus. The different extracts such as hexane, chloroform, ethyl acetate, ethanol, methanol and aqueous extract exhibits comparable antimicrobial activity with the...
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