Posted by admin on Sep 30, 2025 in |
Introduction: In urothelial carcinomas, the degree of invasion plays a major role in prognostication and treatment assessment. In certain instances, evaluating invasion on standard histopathological sections may be challenging. Actin-bundling protein fascin is implicated in the migration of tumour cells and is expressed more intensely in invasive tumours. Objectives: To evaluate the expression of fascin-1 in urothelial carcinoma of urinary bladder and correlate it with different grades of tumor and level of invasion and other clinicopathological parameters. Material and Methods: Observation study was conducted after approval from the institutional ethical committee. Fascin-1 immunoreactivity was studied in 45 confirmed cases of urothelial neoplasms using monoclonal antibody against fascin by immunohistochemistry. The extent, intensity, and combined immunoreactivity score of fascin-1 cytoplasmic immunoreactivity were evaluated semi quantitatively. Results: 29 cases (64.4%) had high grade urothelial carcinoma, 14 cases (31.1%) had low grade urothelial carcinoma, and two cases (4.4%) had papillary urothelial neoplasm of low malignant potential (PUNLMP) based on histological classification. In stage pTa were two cases (4.4%), of stage pT1 were...
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Posted by admin on Sep 30, 2025 in |
This study aimed to assess the anti- anemic activity of Macrotyloma uniflorum grains and Curcubita pepo seeds against Phenylhydrazine- induced anemia in albino rats. It was done by two extraction methods. In extract-1, Macrotyloma uniflorum was collected and soaked in water for whole night and next morning the grains were tied in a clean cloth and left for 3-4 days (Sprouted). The sprouted grains were dried under shade. Then the sprouted grains are fine powdered in a mill. The powder was macerated with 50% of alcohol for 7 days and filtered. The filtrate was evaporated after the complete evaporation of the crude extract. This extract was given to the animals for 14 days and on 15th day, the blood samples were collected and examined. In extract-2, Curcubita pepo was collected, outer shell was removed and dried under shade. The dried material was fine powdered in a mill. The powder was macerated with 50% of alcohol for 7 days and filtered. The filtrate was evaporated. After the complete evaporation of...
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Posted by admin on Sep 30, 2025 in |
The active compounds present in plants gained attention for their biological activity and this makes them important for the pharmaceutical industries. Butea monosperma has significant role in traditional medicines. Its leaves, bark, and flower have an important role against diabetes, filariasis, ulcers, inflammations, etc. However, till date no detailed phytochemicals profiling in flowers of B. monosperma along with their antioxidant and antidiabetic potential has reported. This study aims to measure antioxidant activity, total phenols, flavonoid contents in methanol and ethanol extract of the flower, and to identify their compounds through Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Biological activity was determined by available literature present on NCBI, PubChem. However, Antioxidant actions were measured by DPPH and FRAP assays. Further antidiabetic potential of compound extract was analyzed using in-silico approach. It was found that methanolic extract showed better antioxidant potential than ethanolic extract. Total Phenol and flavonoid content were investigated but no significant difference was observed between the extracts. 42 major compounds were identified in methanolic extract using GC-MS, among which, 30...
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Posted by admin on Sep 30, 2025 in |
Necrotizing Enterocolitis (NEC) is a severe gastro-intestinal disease primarily affecting premature infants, especially those born at or before 32 weeks of gestation, and is associated with high morbidity and mortality rates, characterized by intestinal inflammation and necrosis. The purpose of this study was to investigate the role of genetic factors in the development of necrotizing enterocolitis, mortality cases, and potential links between these two conditions. A total of 44 infants were included in this study. Based on survival during the neonatal period, NEC were divided into two subgroups: those who survived (35 infants) and those who died 9 infants). SIGIRR gene methylations were identified through DNA isolation and standard PCR reactions. It was found that changes in the %C33, %C35, %C51 and %C102 gene loci are potential predictive markers for distinguishing infants who died from NEC from those without this pathology Genetic screening could help identify infants at a higher risk of developing NEC, allowing for timely, targeted intervention and personalized care...
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Posted by admin on Sep 30, 2025 in |
Current study aims to evaluate stability of Rebaudioside M, a sweet component of Stevia rebaudiana, using a typical pH (2.8, 3.2, 3.8 and 4.2) and temperature (5 °C, 20 °C, 30°C, 40 °C) for 26 weeks, range that simulated both relevant and extreme beverage storage conditions. Photostability of mock beverages at pH 3.8 was determined at 25 °C /60% relative humidity (RH) with fluorescent light exposure. Rebaudioside M (Reb M) was evaluated in mock beverage solutions by simulating formulations used in commercial soft drinks and were subjected to various temperature conditions (5 °C, 20 °C, 30 °C, 40 °C) for 26-weeks at each pH. Samples were analyzed at scheduled intervals throughout the 26 weeks for Reb M, known impurities, known degradation products, and unidentified compounds greater than or equal to 0.100% of the starting concentration of Reb M. There was minimal degradation of the Reb M when exposed to fluorescent light, and appearance did not change throughout the study. Thus, Reb M in mock beverages under relevant conditions of...
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