Posted by admin on Sep 30, 2026 in |
Objective: The present study aimed to develop and validate a simple, rapid and environmentally acceptable HPTLC method for the quantitative estimation of bupropion hydrochloride in tablet dosage form and to evaluate its greenness profile using AGREE, GAPI, NEMI and Analytical Eco-scale tools. Methods: Chromatographic separation was achieved on silica gel 60 F254 precoated aluminium plates using methanol: chloroform: glacial acetic acid (1.5:8.5:0.3, v/v/v) as the mobile phase. Densitometric analysis was performed at 252 nm after a chamber saturation time of 15 min. The method was validated according to ICH Q2(R1) guidelines. Results: Bupropion hydrochloride exhibited a compact spot with an Rf value of 0.37 ± 0.001. The method showed linearity over the concentration range of 150–350 ng/band with a correlation coefficient of 0.9984. The assay of marketed tablets was found to be 98.44%. The recovery range was –%, indicating good accuracy. Precision studies showed %RSD values below 2%, confirming method precision. The LOD and LOQ were found to be 7.36 ng/band and 22.31 ng/band, respectively. Robustness studies indicated that...
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Posted by admin on Sep 30, 2026 in |
This study examined the phytochemical composition, bioactivity, and biosafety of the hydroethanolic extract of Cissus quadrangularis, a plant traditionally employed in the management of diabetes mellitus. Phytochemical analysis identified a rich presence of alkaloids, flavonoids, glycosides, and phenolic compounds, with the ethanolic fraction yielding a high total phenolic content (60.96 mg GAE/g). The in-vitro anti-inflammatory potential of the extract was assessed via the egg albumin denaturation assay across concentrations up to 1000 µg/mL and benchmarked against Diclofenac, a standard non-steroidal anti-inflammatory drug. In parallel, acute oral toxicity and biosafety were evaluated in female Sprague-Dawley rats over a 14-day period, with dosage escalation up to 5000 mg/kg body weight. The hydroethanolic extract exhibited significant, concentration-dependent anti-inflammatory activity, attaining a maximum inhibition of69.30±1.57% at 1000 µg/mL, which was comparable to the 83.45±1.27% inhibition observed with Diclofenac. Acute toxicity evaluation indicated an excellent safety profile, with no mortality or adverse clinical signs, and normal behavior, food and water intake, and weight gain observed across all groups, including the highest dose cohort. In...
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Posted by admin on Sep 30, 2026 in |
Background: Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder among women of reproductive age and is frequently associated with obesity, insulin resistance, dyslipidaemia, and impaired psychological well-being. Lifestyle modification remains the first-line approach for PCOS management. Traditional food-based interventions incorporating Garcinia cambogia have attracted increasing attention because of their hydroxycitric acid (HCA) content and potential role in weight management. Objective: To evaluate the effects of a structured traditional dietary intervention incorporating a Garcinia cambogia-based functional food on body composition, biochemical parameters, dietary intake, and psychological well-being among young women with PCOS. Methods: A 12-week single-group pre-post intervention study was conducted among 34 young women aged 19–23 years diagnosed with PCOS according to the Rotterdam criteria. Participants received individualized calorie-controlled dietary plans, weekly nutrition education and lifestyle counselling, and daily supplementation with a Garcinia cambogia based functional bite. Anthropometric measurements, body composition, biochemical parameters, dietary intake, and psychological well-being were assessed at baseline and after completion of the intervention. Hydroxycitric acid content of Garcinia cambogia powder was...
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Posted by admin on Sep 30, 2026 in |
Aim: The present study aimed to develop and validate a simple, rapid, accurate, precise, stability-indicating, and environmentally friendly RP-HPLC method for the quantitative estimation of Vonoprazan Fumarate (VPZ) in pharmaceutical dosage forms and to evaluate its greenness using AGREE-based assessment tools. Study Design: Experimental analytical method development and validation study based on ICH guidelines. Place and Duration of Study: Department of Pharmaceutical Chemistry and Department of Pharmaceutical Analysis, Delonix Society’s Baramati College of Pharmacy, Maharashtra, India. The study was carried out during the academic research period of 2025–2026. Methodology: Chromatographic separation was performed on C18 column (250 × 4.6 mm, 5 µm) with an isocratic mobile phase of methanol and aqueous phase containing 1% HCl (70:30 v/v) at a flow rate of 1.0 mL/min. Detection was carried out at 268 nm. The developed method was validated for specificity, linearity, accuracy, precision, robustness, ruggedness, limit of detection (LOD) and limit of quantification (LOQ) as per ICH requirements. Forced degradation studies were performed under acid, base, oxidative, thermal and photolytic stress...
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Posted by admin on Sep 30, 2026 in |
A series of rare-earth metal complexes incorporating a Kynurenic acid ligand were successfully synthesized and characterized using elemental analysis, molar conductance measurements, and spectroscopic techniques, including FT-IR, UV–Visible, and Mass spectroscopy. The analytical and spectral data confirmed the coordination of the ligand to the metal ions through the donor atoms, leading to the formation of stable coordination compounds. The antimicrobial activity of the synthesized complexes was evaluated against selected Gram-positive and Gram-negative bacterial strains as well as representative fungal pathogens using standard in-vitro assays. Comparative studies revealed that the metal complexes exhibited significantly enhanced antimicrobial activity relative to the free ligand. The observed improvement in biological efficacy may be attributed to increased lipophilicity and facilitated penetration of the complexes through microbial cell membranes upon chelation. Furthermore, variations in antimicrobial performance among the complexes indicate that the nature of the rare-earth ion and the coordination environment play important roles in determining biological activity. The findings demonstrate that quinoline-based rare-earth metal complexes represent a promising class of bioactive compounds with potential...
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