Posted by admin on Jul 31, 2015 in |
Objective: Immune-compromised patients are vulnerable to microbes, which are usually safe and sound for healthy individuals. The aim of this study is to provide a quality fresh fruits such as apple, grape, guava, pear and plum for immune-compromised personnel sing different doses of gamma radiation. Methods: Irradiation impacts on microbial number, in treated fruits were assessed and compared to sanitary microbial safety criteria for immune-compromised patients as suggested by IAEA. Fresh-cut fruits were exposed to various doses of gamma radiation (0, 0.5, 1.0 and 1.5 kGy) and microbial load was analyzed. Results: Microbiological analysis showed that aerobic plate counts in case of guava, grape and pearwere4.53, 3.42 and 3.24 log CFU/g respectively which were eliminated at 1.0 kGy. Aerobic spores, except apple, were totally eliminated just at 0.5 kGy. Similarly, at 0.5 kGy, total Coli form in plum, pear, guava and apple, which were 4.43, 2.25, 2.0 and 1.04 CFU/g respectively, as well as pathogenic Listeria spp. 3.3 log CFU/gin guava were also eradicated. Staphylococcus aureus were detected only...
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Posted by admin on Jul 31, 2015 in |
Ghaafis (Gentiana olivieri Griseb) has been recommended in Classical Unani literature as an important ingredient of many antipyretic formulations. Singularly, it is advocated in both crude and extract form. The present study was conducted to investigate the antipyretic activity of crude and aqueous extract of Gule Ghaafis on yeast induced pyrexia in animal model. The study was carried out by the method described by Vogel. Animals were divided into six groups of six animals each. Group I received distilled water, Group II was given subcutaneous injection of 15% suspension of Brewer’s yeast, Group III and IV were injected subcutaneous injection of 15% suspension of Brewer’s yeast, in the dose of 10ml/kg followed by powder of Gule Ghaafis in the dose of 600mg/kg and 1200mg/kg, respectively. Group V and VI were given subcutaneous injection of 15% suspension of Brewer’s, in the dose of 10ml/kg followed by aqueous extract of Gule Ghaafis in the dose of 162 mg/kg and 324mg/kg, respectively. The rectal temperature of the animals in different groups was...
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Posted by admin on Jul 31, 2015 in |
Introduction: Khat is chewed traditionally in Ethiopia, Somalia, Yemen and Kenya. In Ethiopia, khat chewing is now becoming an every-day substance of abuse for the general population regardless of its adverse effects on human health. Therefore, this study was conducted to investigate the effects of crude extract of khat fresh leaves and soft twigs on liver function in rats. Methods: Adult male Wistar rats (N = 35) aged between 14 to 16 weeks were randomly allocated into five groups of 7 animals each. Rats in group I were given 1mL of distilled water, and served as control. Rats in group II, III and VI were treated with crude extract of 150, 250, 350 and 450 mg/kg doses of khat respectively. At the end of the experiment, blood samples were collected and serum level of liver enzymes, total protein and bilirubin were determined. Results: Serum level of AST, ALT and ALP were significantly elevated while the total protein was significantly decreased (P < 0.05). The total and direct bilirubin were...
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Posted by admin on Jul 31, 2015 in |
Liver defend the PAMPs or DAMPs via regulation of innate immunity through modulation of “TOLL LIKE RECEPTORS and NOD LIKE RECEPTORS” A breakdown of tolerance may induce in appropriate immune response leads to acute and chronic liver diseases like NASH/NAFLD thus considered as disorders of immune response. HFD is itself a basic component for generating metabolic disorders and major source for formation of endotoxins in body through leaky gut and sensitization of the TLR4. NAFLD is one of the groups of disease which links the innate immunity through the metabolic disorder. Agonist of TLR4 – LPS is the primary and NLRP3 agonist MDP is secondary signal transducer for the sensitization of the Caspase activation cascade and TNFα activation serves as the proinflammatory cytokines in disease like NAFLD. The present study was elucidated to develop combinations of Lipopolysaccharide and Muramyl dipeptide induced experimental model of NAFLD in HDF fed rats. Various parameters such as body weight, SGOT, SGPT, ALP, Direct Billirubin, serum triglyceride, weight of liver, and ratio of liver...
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Posted by admin on Jul 31, 2015 in |
Objective: The current study was designed to study Acute Dermal Irritation potential of Mastilep gel (supplied by M/S Ayurvet Limited, Baddi, India) according to OECD guidelines. Materials and Methods: Mastilep gel, a new herbal ointment for topical use applied on the udder, has been developed as an alternative method for controlling mastitis in ruminants. Mastilep gel’s active ingredients including herbal extracts of Cedrus deodara, Curcuma longa, Glycyrrhiza glabra and Eucalyptus globulus are expected for their antibacterial, anti-inflammatory, analgesic, antihistaminic and immunomodulatory effects. For new substances it is the recommended stepwise testing approach for developing scientifically sound data on the corrosivity/irritation of the substance. Three female rabbits were used for the study. Each animal served as its own control. After application of Mastilep gel the degree of irritation/corrosion was read and scored. Results: No severe erythema, oedema or any skin lesion was observed after Mastilep gel application. The results revealed no irritation potential of Mastilep gel. Conclusion: MASTILEP gel is safe for...
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