Posted by admin on Jun 30, 2015 in |
Strychnos potatorum Linn. is a medium sized deciduous tree belonging to the family Loganiaceae. The collected seeds of Strychnos potatorum were dried and powdered. Methanolic extract of these seeds was prepared by cold maceration. The preliminary phytochemical screening of the crude extract revealed the presence of flavonoids and phenolic compounds. The crude extract was partitioned (gradient) with different solvents like chloroform, ethyl acetate, acetone and water. After conducting preliminary in vitro DPPH free radical scavenging assay on these fractions, the one with maximum antioxidant activity (bioactive fraction) was selected and screened with different in vitro antioxidant methods. The different in vitro antioxidant methods include DPPH free radical scavenging assay, nitric oxide radical scavenging assay, superoxide radical scavenging assay, hydroxyl radical scavenging assay and inhibition of peroxide formation method. The percentage inhibition of free radicals in each method was compared with standard drug. Ascorbic acid and α- tocopherol were used as standard drugs. The seeds of Strychnos potatorum have shown significant antioxidant...
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Posted by admin on Jun 30, 2015 in |
Pulsatile system is gaining a lot of interest as it increases patient compliance by means of providing time and site specific drug delivery system. This work will aim at the design of a sustained release formulation with potential use of metoprolol tartrate capsule which is used in the treatment of hypertension. The design involves the preparation of cross linked hard gelatin capsules by using formaldehyde, then the drug diluents mixture loaded in, which was separated by using hydrogel plugs of different polymers, such as, HPMC 15cps, HPMC 50cps, HPMC 100cps, HPMC K4M, HPMC K15M, HPMC K100M, xanthan gum, carbomer 940, carbopol 971, sodium CMC and sodium alginate of two different amounts (75mg and 100mg respectively). Prepared formulations were subjected to evaluation of various phycal parameters and in vitro drug release studies. From the in vitro dissolution studies it was found that by increasing the amount of polymer, release rate was decreased. 75mg of HPMC 15cps showed 91.23% drug release in 5hr whereas 100mg showed 96.68% drug release in 7hr....
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Posted by admin on Jun 30, 2015 in |
In present study the electro-oxidative behaviour and determination of meropenem at a glassy carbon electrode were investigated using cyclic, differential pulse anodic adsorptive stripping voltammetry (DPAAdSV) and square wave anodic adsorptive stripping voltammetric (SWAAdSV) techniques. The dependence of the peak current and peak potentials on pH, concentration and scan rates were examined. The oxidation of meropenem gave a well-defined single irreversible peak at glassy carbon electrode vs Ag/AgCl (3M KCl) reference electrode. The oxidation process was diffusion controlled. A linear response was obtained between 1.0 x10-6to 4.0x 10-5 M in aqueous media for differential pulse and square wave techniques. The recovery studies were also achieved to check sensitivity and selectivity of the proposed methods. The proposed methods were applied for the determination of meropenem from pharmaceutical dosage forms without any time-consuming extraction, separation, adsorption steps. The value of LOD was achieved 1.27×10-7 M and 2.24×10-7 M for DPAAdSV and SWAAdSV...
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Posted by admin on Jun 30, 2015 in |
Cassia sieberiana and Khaya grandifoliola are two plants from Ivorian traditional pharmacopoeia. In this study, we have compared in vitro antibacterial potential of glycosidic and aglyconic crude extracts from both plant species against 3 reference bacterial strains (Escherichia coli ATCC 25922, Pseudomonas aeruginosa 27853, Staphylococcus aureus ATCC 25923) and 4 others clinical (Proteus mirabilis, Salmonella sp, Shigella sp, Vibrio cholerae) isolated from pathological specimen at hospital. The results have revealed on all tested bacteria, a clear antibacterial potential of aglycones compared to glycosides. Indeed, at same concentrations, total aglycones of each plant have inhibition zones more extensive than their corresponding glycosides, and their MIC are clearly lower against bacterial strains. Moreover, we note that the aglycones are active against Escherichia coli ATCC 25922 and Pseudomonas aeruginosa 27853 while when the glycosides are inactive. This suggests that in the glycoside, the sugar moiety inhibits the antibacterial...
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Posted by admin on Jun 30, 2015 in |
New simple and sensitive extractive spectrophotometric methods have been developed and described for the determination of carisoprodol in pure form and in pharmaceutical formulations. The developed methods involve formation of coloured chloroform extractable ion-pair complexes of carisoprodol with triphenyl methane dyes viz., bromocresol green (BCG), bromothymol blue (BTB) and bromophenol blue (BPB) in acidic medium. The extracted complexes showed absorbance maxima at 420, 417 and 413 nm with use of BCG, BTB and BPB respectively. The stoichiometry of the complex in three cases is found to be 1:1. Beer’s law is obeyed in the concentration ranges: 2.5-25, 2.5-30 and 3.0-30μg/ml with BCG, BTB and BPB respectively. The effect of concentration of dye, pH and interference of excipients have been studied and optimized. The limits of detection and quantification have been determined for all the three methods. These methods have been validated as per the guidelines of International Conference on Harmonization (ICH) and have been applied to the determination of carisoprodol in commercial tablets and results of analysis were validated...
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