Posted by admin on Oct 31, 2015 in |
This paper describes a simple, precise, and validated high-performance liquid chromatographic method for the simultaneous quantitative determination of Acetylcysteine and Cefixime in pharmaceutical formulation in the presence of degradation products. The separation was achieved using a Kromasil C8 reverse phase column (25X 0.46mm I.D, 5µ particle size) at room temperature with an isocratic mixture of methanol:KH2PO4 (50mM, pH 3.0) (30:70) at a flow rate of 1ml/min and detection at 228 nm. To establish stability indicating capability of the method, drug product was subjected to the stress conditions of acid, base, oxidative, hydrolytic, thermal and photolytic degradation. The degradation products were well resolved from Acetylcysteine and Cefixime. The developed method was validated as per ICH guidelines with respect to specificity, linearity, accuracy, precision and robustness. The developed methodology is an economic, time-saving, straight forward and precise assay for the determination of Acetylcysteine and Cefixime in pharmaceutical preparation. It can be readily utilized for Quality Assurance (Q.A) and Research and Development (R&D) laboratories of pharmaceutical...
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Posted by admin on Oct 31, 2015 in |
Cancer is a common and widespread cause of human death in the world. Treatments like chemotherapy and radiotherapy facilitate in prolonging the patient’s life however in some cases cancer can be prevented. Cancers like breast, prostate, colon, renal cell carcinoma are caused by the overexpression of an enzyme termed Carbonic Anhydrase IX (CAIX). In 2015, 60,290 new cases of breast cancer alone are estimated. If this overexpression is controlled using an inhibitor, then the mortality rates due to cancer can be brought down by a significant margin. The primary objective of this research is to find an inhibitor that would reduce or down regulate the overexpression of CAIX in the human body. The inhibitor would specifically target CAIX. CAIX has a region which is unique and its cavities were identified. These were docked with the chosen inhibitor. Numerous acetazolamides and sulfanilamides were tested as inhibitors. Various bioinformatics tools such as Maestro and Hex were used for docking and grid generation. Docking scores were obtained and studied. The inhibitors with...
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Posted by admin on Oct 31, 2015 in |
Objectives: The present study was conducted to evaluate and compare the effect of vanadium pentoxide and chromium oxide in normal and streptozotocin induced diabetic albino rats. Methods: Diabetes was experimentally induced by injecting intraperitoneally with a single dose of 60mg/kg. The animals were considered as diabetic, if their blood glucose values were above 300 mg/dl on the 10th day after injection. The blood glucose estimation was done by glucose oxidase method. Glibenclamide was taken as standard drug. The one-way ANOVA followed by Dunnett’s ‘t’ test was used for statistical analysis. Results: The blood glucose levels were found to be significantly (p<0.05) decreased in vanadium pentoxide and chromium oxide treated groups. Conclusion: This study suggests that vanadium pentoxide and chromium oxide have antidiabetic effect in a dose dependent...
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Posted by admin on Oct 31, 2015 in |
Sophisticated analytical methods like HPLC and Mass spectroscopic methods which being employed for analysis, are relatively expensive and hence there is a need for developing simple analytical methods. In the proposed work, such methods have been developed and applied for routine determination of ketorolac in pharmaceutical formulations and bulk dosage forms. These methods were based on the formation of colored species on binding of ferric chloride and Hydrochloric acid for method A and binding of drug and Folin Cioclataeu reagent in alkaline conditions for method B, and the colored chromogen obtained in each method was finally treated with the drug ketorolac to produce yellow colored for method A and blue colored chromogen for method B with λmax at 430 nm and 627 nm respectively. Statistical analysis of these methods exhibited Sandell’s Sensitivity of 0.8 and 0.0617 (Method A and B) respectively, and the relative standard deviation (RSD) of these methods were found equal to 0.69 and 1.90 respectively, indicating that these methods are highly reproducible, based on the principle...
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Posted by admin on Oct 31, 2015 in |
The term HbA1c refers to glycated haemoglobin. By measuring glycated haemoglobin (HbA1c), clinicians are able to get an overall picture of what our average blood sugar levels have been over a period of weeks/months. Diabetic neuropathy is generally assessed in clinical practice by a combination of objective and subjective measures. Nerve conduction studies, the distal sural sensory nerves and the bilateral common peroneal and posterior tibial motor nerve was performed in lower limb in female patient of 20-years old suffering from type-I diabetes from past 10-yeras and had poor glycemic control (haemoglobin [HbA1c] 9.56%). Studies showed motor conduction studies (MNCV) and sensory conduction studies (SNCV) of the lower extremities, revealed borderline- prolonged distal latencies, evoked CMAPs of reduced amplitude of common peroneal, posterior tibial (right and left) with reduced conduction velocity was observed and however sural SNAP amplitude recordings in lower limb was normal. F response latencies were markedly prolonged in patient. Electro-diagnostic techniques play an important role in the prognosis of diabetic neuropathy because timely intervention reduces disability and...
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