Posted by admin on Jul 31, 2015 in |
A reverse phase liquid chromatography method (RP-HPLC) was developed to estimate the amount of Indapmide and Perindopril in bulk and its pharmaceutical formulations. A Waters Alliance HPLC system equipped with auto sampler, UV-Visible detector and YMC Column (150 x 4.6mm, 3µ particle size) were used for the quantification of the drugs. Separation was carried out by using potassium dihydrogen phosphate buffer of pH 2.5 and acetonitrile in the ratio 60:40 v/v as mobile phase at a flow rate of 1 ml/min and the detection was carried out at a wavelength of 230 nm. The retention time, tailing factor and USP theoretical plates were found to be 2.5 min., 1.38 and 2551 for Indapamide and 4.18., 1.75 and 2778 for Perindopril respectively. The area of the peak was proportional to the concentration of the drug in the range 15-35, 48-112 μg/ml of Indapmide and Perindopril. The values of LOD and LOQ for Indapmide and Perindopril were found to be 0.07, 27.87 and 0.23, 84.47 μg/ml respectively. The mean recoveries of...
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Posted by admin on Jul 31, 2015 in |
In the present investigation, controlled release microparticles of Glimepiride were prepared by emulsion solvent evaporation technique using cellulose acetate as release retardant polymer. The microparticles were evaluated for In-Vitro release study and In-Vivo evaluation. The microparticles were subjected to In-vitro release studies by employing 7.8 pH phosphate buffer as dissolution medium. The rate of drug release from these microparticles followed zero order kinetics and the mechanism of drug release was governed by peppas mechanism. The exponential coefficient (n) value indicating that drug release followed non fickian mechanism. The in vivo performance of pure drug and optimized Glimepiride microparticles were evaluated in rabbits in a randomized cross-over design. The estimation of glimepiride in serum was carried out by HPLC method. The parameters such as maximum plasma concentration (Cmax), time for peak plasma concentration (tmax), mean residence time (MRT) and area under curve (AUC0 – α) were significantly (P< 0.001) differed following glimepiride microparticles compared to pure drug administration. The relative bioavailability of glimepiride was increased about five fold after microparticles...
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Posted by admin on Jul 5, 2015 in |
Alpinia galanga Linn. (Zingiberaceae) presents many chemical constituents of the volatile oil extracted from the rhizome. The rhizome of Alpinia galanga is used by people in many regions for relieving toothache, abdominal pain, muscular swelling and rheumatism. This study was performed to determine the antinociceptive and anti-inflammatory activities of a methanolic extract of Alpinia galanga. Analgesic activity was evaluated by acetic acid induced writhing responses, tail flick and hot plate methods in mice. In analgesic activity the A. galanga extract significantly reduced the writhes and increased the tail withdrawal time and paw jumping in albino mice. The anti-inflammatory activity was evaluated by carrageenan induced edema in wistar albino rats. It was found that the extract inhibits the paw edema significantly (p<0.05) at both 250 mg/kg and 500 mg/kg dose levels when compared with control. The positive control drugs for analgesic and anti-inflammatory studies were employed as morphine and indomethacin, respectively. The present findings indicate the antinociceptive and anti-inflammatory effect of A. galanga extract in...
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Posted by admin on Jul 5, 2015 in |
Resurgence of public interest in traditional medicine is increasing in both the developing and developed countries. Myristica fragrans belonging to the family Myristicaceae is commonly known as jaifal and even used as home-remedies in the rural parts of the India. The fruit is used as traditional medicine more frequently for the treatment of common ailments of children in rural folklore practices at the place. Work on standardization assumes vital significance. However, no supportive data available on microscopic characteristics and standardization of the same. In this article a study has been made to fix the parameters includes botanical identification, physicochemical analysis, preliminary phytochemical analysis, HPTLC fingerprints profile, microbial screening, aflatoxin and heavy metals in order to ensure the use of only genuine and uniform material of such herbal remedies. The obtained values/ranges can be used as standards for quality control of the...
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Posted by admin on Jul 5, 2015 in |
Purpose: Bronchial Asthma is a chronic disease that affects the airways. Lung function declines faster than average in people with asthma. Overall, one study reported that 72% of men and 86% of women with asthma had symptoms 15 years after an initial diagnosis. Only 19% of these people, however, were still seeing a doctor, and only 32% used any maintenance medication. Hence a study was conducted to determine the level of medication adherence and factors associated with non adherence among patients with bronchial asthma. The objectives of the study were to assess the level of medication adherence among patients with bronchial asthma and identify the reason for non adherence to prescribed therapy and associate the level of medication adherence with the selected demographic variables. Methods: A Qualitative study design was adopted and the study was conducted in Chest OPD, Sri Ramachandra Hospital, Chennai, Tamilnadu. A total of 60 patients were selected for the study by using convenient sampling technique. Results: The result suggest that 37 (61%) has low level...
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