Posted by admin on May 30, 2015 in |
The present study deals with the evaluation of the antimicrobial activity in methanolic extracts of in vivo (leaf, stem and seed) and in vitro (callus) plant parts of Merremia dissecta and Merremia aegyptia belonging to family Convolvulaceae. The antimicrobial activity against six different microbial strains, four bacterial viz. Bacillus thuringiensis, Bacillus subtilis, Eschrechia coli and Pseudomonas putida and two fungal strains viz. Candida albicans and Aspergillus niger was studied. Initially, plant extracts prepared in four different solvents (Aqueous, Methanol, Ethanol, and Petroleum Ether) were screened for their antimicrobial activity through TTZ test (Tetrazolium Chloride Test). It was assessed that alcoholic sample extracts possessed highest antimicrobial activity amongst all. Later the antimicrobial activity of the methanolic extracts was confirmed using Disc-Agar Diffusion technique. The inhibition zone observed was representative of the antimicrobial potency of that particular sample extract. Zones of inhibition ranging from 6mm to 14mm were observed. The largest zones of inhibition were observed from the leaf extracts of these plants and the leaf extract of Merremia aegyptia also...
Read More
Posted by admin on May 30, 2015 in |
In the present study the anti-arthritic activity of ethanolic extracts of leaf and seed of Elaeocarpus serratus L. was evaluated in vitro using two models namely on the inhibition of protein denaturation and anti-proteinase activities. Indomethacin was used as a standard drug. Results revealed that the ethanolic leaf and seed extracts of E. serratus at two different concentrations (200 and 400 µg/ml) possessed notable activity as compared to the standard drug indomethacin, justifying its usefulness in the treatment of inflammation associated diseases like...
Read More
Posted by admin on May 30, 2015 in |
Diaporthephaseolorum is endophytic fungi isolated from Jatitree (Tectonagrandis L.f). Endophytic microbes were microbes that spent part or all of their life live within plant tissue of a host plant. They produce secondary metabolites with potent anti microbial activity. Jatitree (Tectonagrandis L.f) was one of herbal plants that act as host plants for endophytic microbes that produce secondary metabolites with anti-microbial activity. The current study focussed on isolation of the endophytic microbes from branches of Jati tree and their secondary metabolites. Isolation of endophytes were performed in PDY (potato dextrose yeast) and further extracted using EtoAC. Four fractions (F I – F IV) was obtained from EtOAc extract through column chromatography (n-Hex: EtoAc, ratio 50:1). Further, agar diffusion method was performed to assess their anti-microbial activity. Results demonstrated that F IV has potent towards Staphylococcus aureus (11.10mm). Its GC-MS profile strongly indicated the presence of fatty acid and phenolic related compound. In conclusion, secondary metabolites of endohpytes isolated from Jati tree branches contained predominantly phenol and fatty acid related compound...
Read More
Posted by admin on May 30, 2015 in |
In this present study, the leaf extracts of Citrus hystrix were subjected to a comparative evaluation of preliminary phytochemical screening, membrane stabilizing activity, thrombolytic activity and In-vitro antioxidant activity. The thrombolytic and membrane stabilizing activities were assessed by using human erythrocyte and the results were compared with standard streptokinase (SK) and standard anti-inflammatory drug, acetyl salicylic acid (ASA), respectively. For thrombolytic activity and membrane stabilizing activity ethanolic leaf extract exhibited clot-lysis value of 13.69% against standard SK (37.43 %) and highest percentage of hemolytic value of 74.40% against standard ASA (93.24%) respectively. DPPH free radical scavenging activity, Cupric Reducing Antioxidant Capacity (CUPRAC), Total Phenolics Content, Total Flavonoids Content, Nitric oxide scavenging assay and total antioxidant capacity were done in the present study. In case of in-vitro antioxidant evaluation all extract forms showed a significant positive results. Phytochemical investigation suggested the presence of alkaloids, carbohydrates, flavonoids, glycosides, phenols, steroids and...
Read More
Posted by admin on May 30, 2015 in |
In the present work a simple, accurate and precise method has been developed and validated for the simultaneous estimation of Perindopril Erbumine (PDE), Amlodipine Besylate (AMD)and in their combined dosage form by UV Spectrophotometric Methods. The Method A employs estimation of drugs by simultaneous equation method (SEM) using 298.3 nm and 231.9nm i.e. λmax values of PDE and AMD respectively. Method B employs the estimation of drugs by Absorption Correction method (ACM) at 250.4nm for PDE where absorbance of AMD is zero. Absorbance correction method was based on the property of additivity of absorbances. At 250nm, Perindopril erbumine showed some absorbance while Amlodipine besylate showed zero absorbance. The method involved measurement of absorbance at two wavelengths 250 and 231 nm. For estimation of AMD, corrected absorbance was calculated at 231 nm due to the interference of PDE at this wavelength. Calibration curves were linear with correlation coefficient between 0.999 over the concentration range of 10-150μg/mL and 0.5-10 μg/mL for PDE and AMD respectively. The percent recoveries of the drugs...
Read More
