Posted by admin on May 30, 2015 in |
The antioxidant potential of the aqueous and hydro/ethanolic (ethanol/water) extracts of Crinum jagus were explored considering their total phenolic, flavonoid and proanthocyan contents, 1.1-Diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, ferrous ions chelating ability and nitric oxide radical inhibiting activity. Hydroethanolic extract has exhibited the highest total polyphenols and flavonoids content as well as the highest DPPH inhibition value (56.71 ± 0.05 % at 320 µg/mL) and ferrous ions chelating ability (60.25 ± 0.22 % at 6 mg/mL), when the aqueous extract showed the highest proanthocyanes content, nitric oxide inhibiting activity comparable to that of ethanol/water extract (respectively 64.52 ± 0.03 % and 65.90 ± 0.43 % with 320 µg/mL extract). The IC50 values of the DPPH and the ferrous ions inhibition were respectively 25.21 µg/mL and 4.69 mg/mL; the IC50 value of nitric oxide inhibiting ability was 16.48 µg/mL and 16.14 µg/mL for the aqueous and hydroethanolic extracts respectively. The results indicate that, Crinum jagus might possess effective in vitro antioxidant activity which could be more marked with ethanol/water...
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Posted by admin on May 30, 2015 in |
Unani and Ayurvedic heath care system is considerable areas of medical treatment in Bangladesh where patient can avail it from different Govt. or private hospital. This cross-sectional study was undertaken to find out the satisfaction of patients attending in this health care system from January to April 2014. A total of 240 respondents were interviewed with an interviewer administered semi-structured questionnaire. Among the respondents majority (54.2%) of them was aged ranging from 21- 40 years. The study revealed that more than 71% respondents were married and majority of them (55.4%) have history of use of Unani/Ayurvedic medicine before. The study found that majority (79.6%) of the respondent has found satisfactory and good quality behavior from the doctor of these systems and it was also found that a good number of respondents (more than 80%) commented doctor’s service as satisfactory and good. It was found that more than 80% respondent got satisfactory and good behavior from pharmacist and concerned stuff of the hospital. Regarding health service delivery system of the...
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Posted by admin on May 30, 2015 in |
The leaves and fruits of Smilax perfoliata are traditionally used for treatment of various ailments such as rheumatism, lumbago, nourishing the functions of spleen, stomach, muscle and bone. The intent of the present study was to evaluate the antioxidant and anti-ulcer activity of ethanolic leaves and fruits extract of Smilax perfoliata Linn in albino rats. Gastric ulcers were induced by employing pylorus ligation method of induction. The anti-ulcer activity was analyzed by estimation of free acidity and the total acidity. The antioxidant potential was analyzed by method of DPPH free radical scavenging activity. The results obtained showed that the leaves and fruits of Smilax perfoliata possess antioxidant potential and are capable in the ministration of gastric...
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Posted by admin on May 30, 2015 in |
In the present study, the hydro-alcoholic and hexane extracts of the plant, Valeriana jatamansi (Family: Valerianaceae) were screened for antimicrobial activity against pathogenic and drug resistant strains. The non polar extracts did not showed any activity against any of the pathogenic strains. Amongst these extracts the hydro-alcoholic extracts of the plant, Valeriana jatamansi, showed potent antimicrobial activity against almost all the pathogens studied. These extracts also showed potent antibacterial activity against multidrug resistant Staphylococcus aureus and Pseudomonas aeruginosa in the ranges from 0.3 mg/ml to 0.7 mg/ml. The potent hydro-alcoholic extract was further subjected for isolation and characterization of potent antimicrobial compound via chromatographic and spectroscopic techniques. The pure compound isolated, Valerenic acid was obtained as colorless liquid with a penetrating aroma; slightly soluble in water, soluble in alcohol, and ether. It melts at -34 0C and boils at 186 0C. The compound showed Rf value 0.37 similar to that of the standard compound, Valerenic acid as detected by TLC. The retention time of the pure compound purified was...
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Posted by admin on May 2, 2015 in |
A simple, rapid, precise, cost effective, stability indicating RP-HPLC method has been developed and validated for the simultaneous estimation of Doxycycline monohydrate (DOXM) and Ornidazole (ORN) in Bulk and pharmaceutical dosage form. The chromatographic separation was achieved on Hypersil BDS C18 column (250mm ×4.6mm, 5μm) using a mobile phase consisting of Buffer : Acetonitrile (55:45 v/v) pH 4 adjusted with ortho phosphoric acid at a flow rate of 1ml/min. Detection wavelength was found 260 nm. The retention time found for the drugs DOXM and ORN were 2.8 min and 4.3 min. respectively. The linearity of the method was over the range 12.5 – 75 µg/ml and 62.5– 375 µg/ml for DOXM and ORN, respectively. The validation of method was carried out utilizing ICH guidelines. The described RP-HPLC method was successfully employed for the analysis of pharmaceutical formulations containing combined dosage form. Developed HPLC method can resolve all degradant peaks of both the drugs, so this method is stability indicating in...
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