Posted by admin on Apr 30, 2015 in |
The objective of the present study was to i) Extract ursolic acid , a pentacyclic triterpenoid from Ocimum sanctum; ii) develop a HPTLC based method for its quantification; and iii) investigate ursolic acid dependent adaptive response to cytotoxic effect of hydrogen peroxide. To elucidate the possible underlying mechanism(s), lymphocytes were co-incubated with hydrogen peroxide (0, 10, 25 and 100 mM) and ursolic acid (20 and 40 mg/ml incubation medium). After 2 h of incubation we investigated the profile of DNA strand breaks, intracellular reactive oxygen species (ROS), apurinic/apyrimidinic sites (AP sites) and 8-hydroxy-2′-deoxyguanosine (8-OH-dG) residues in DNA. Results showed that ursolic acid inhibited hydrogen peroxide induced DNA strand breaks. Ursolic also showed significant free radical scavenging as indicated by reduced ROS and decreased AP sites. The formation of 8-hydroxy-2′-deoxyguanosine (8-OH-dG) in lymphocyte DNA was also inhibited. These findings clearly showed mechanistic basis for protective effect of ursolic acid against intracellular ROS and consequent generation of AP sites in genomic DNA. In conclusion, our studies clearly suggest that ursolic acid...
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Posted by admin on Apr 30, 2015 in |
Objective– To evaluate the antidepressant activity of chronic administration of Tramadol alone and when combined with Fluoxetine in low doses in albino mice. Methods– The antidepressant effect was determined by recording the immobility time in Despair Swim Test. The mice were randomized into seven groups, with six mice in each group. Group 1 mice were given normal saline (10 ml/kg) which served as control. Mice of groups 2, 3 and 4 received Tramadol in graded doses (10, 20 and 40 mg/kg). Fluoxetine (standard drug) was given to mice of groups 5 and 6. Tramadol and Fluoxetine in low doses (10ml/kg each) were co-administered to mice of group 7.All drugs were given intraperitoneally for 7 days and were subjected to despair swim test on the day 7 of drug administration. Results– Tramadol and Fluoxetine have shown significant antidepressant activity when compared to the control. There is dose dependant increase in antidepressant activity of Tramadol. The antidepressant of Tramadol 20 mg/kg was comparable to Fluoxetine 20 mg/kg. Tramadol potentiated the antidepressant...
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Posted by admin on Apr 30, 2015 in |
Spices are the potential source of natural products and naturally derived compounds. These compounds show antioxidant, antimicrobial properties. The aim of this study is to evaluate the antimicrobial properties of Cinnamomum zeylanicum (Cinnamon). In this study, antimicrobial activities of 70% ethanolic extracts of Cinnamomum zeylanicum were evaluated against different bacterial strains (E.coli, Pseudomonas aeruginosa, Candida albicans) by agar well diffusion method & MIC determination by broth dilution method. The extract showed a broad spectrum of antimicrobial activities by inhibiting the growth of respective microorganism in Agar well diffusion assay. The present study supports the immense medicinal properties of Cinnamomum zeylanicum. It may be insight for future researches in area of new drug development of herbal...
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Posted by admin on Apr 30, 2015 in |
The objective of present study was to formulate and evaluate Tariquidar bimesylate subcutaneous in situ implants in order to minimize the frequency of doses and toxicity and to improve the therapeutic efficacy. The anthranilic acid derivative, tariquidar (XR9576), is a potent and selective P-gp inhibitor being developed clinically for the treatment of multidrug resistant tumors. Tariquidar bimesylate in situ implants were prepared by polymer precipitation method using two different grades of polymer poly (Lactide -co-glycolide) (PLGA) with three different concentrations. The preparation involves dissolving the biodegradable polymer poly (Lactide -co-glycolide) PLGA 85:15 and PLGA 75:25 in N-methyl-2- pyrrolidone. To this solution drug was added and the polymer drug solution was injected in to the aqueous mediato forma solid implant. Based on initial burst release, optimized formulations were selected and evaluated for surface morphology, in vitro drug release and accelerated stability studies. It was observed that the formulations with more polymer concentration and in combination with polyethylene glycol(PEG) and Benzy l benzoate (BB) showed increased entrapment efficiency and exhibited moderate...
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Posted by admin on Apr 30, 2015 in |
The objective of present study is to develop and validate a method for the estimation of sucralfate and oxetacaine in suspension dosage form. An isocratic reversed phase high performance liquid chromatographic method was developed to estimate sucralfate and oxetacaine using thermohypersil BDS (4.6 x 150mm, 5). The mobile phase was prepared by mixing ammonium acetate (pH- 4.4) and methanol in the ratio of (60:40 v/v) which is run isocratically at the flow rate of 0.8ml/min. the wavelength at which detector (PDA) was set is 263nm. The retention time of oxetacaine and sucralfate is 2.08 and 3.4 minutes. Linearity was observed in the concentration range of 20-60g/ml for oxetacaine and 1000-3000g/ml for sucralfate. The percent recovery of sucralfate and oxetacaine was found to be 100. The limit of detection of oxetacaine and sucralfate is 0.178 g/ml and 2.93 g/ml and the limit of quantification of oxetacaine and sucralfate is 0.541 g/ml and 8.879 g/ml. The formulation was subjected to acid, base, peroxide, sunlight and heat and stressed samples were analysed...
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