Posted by admin on Mar 30, 2015 in |
An efficient and sensitive RP-HPLC method was developed and validated for the estimation of related substances in Eletriptan hydrobromide drug substance which were identified and characterized by LCMS, FTIR, 1HNMR, 13CNMR techniques. The method was carried out on a Purosphere STAR RP-8e column at temperature 20°C using a phosphate buffer at pH 4.0 and solvent mixture consists of acetonitrile, water and tetrahydrofuran in the ratio of 70:30:1v/v/v in gradient mode of pump. The flow rate is 1.5 ml/min and detection was done at 225nm.The developed RP HPLC method was validated by testing specificity, precision, detection limit, quantification limit, linearity, accuracy, robustness and range. The linearity of the method was confirmed over the range of 0.09 to1.4µg/ml for related substances with correlation coefficients greater than r = 0.999. The accuracy of the method was found to be 98.6 to 102.3% and RSD as found to be less than 2.0% indicating high degree of accuracy and precision for the proposed method. The effective recovery and lower %RSD prove the highness of...
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Posted by admin on Mar 30, 2015 in |
The main objective of present investigation is to formulate the sustained release tablet of lamotrigine using 32 factorial design. Lamotrigine, a weakly basic and anticonvulsant drug belongs to BCS Class II, having high permeability and poor solubility. The sustained release tablets of lamotrigine were prepared employing different concentrations of HPMC K4M and HPMC K100M in different combinations as a rate retarding polymer by wet granulation technique using 32 factorial design. The quantity of polymers, HPMC K4M and HPMC K100M required to achieve the desired drug release was selected as independent variables, X1 and X2 respectively whereas, time required for 10% of drug dissolution (t10%) and 50% (t50%) were selected as dependent variables. Totally nine formulations were designed and are evaluated for hardness, friability, diameter, thickness, % drug content, In-vitro drug release. From the Results it was concluded that all the formulation were found to be within the Pharmacopoeial limits and the In-vitro dissolution profiles of all formulations were fitted in to different Kinetic models, the statistical parameters like intercept...
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Posted by admin on Mar 30, 2015 in |
Diabetes mellitus (DM) is a complex metabolic disease characterized by chronic hyperglycaemia commonly associated with oxidative stress. The current study aims at assessing the antioxidant and antidiabetic potential of various concentrations of hydroalcoholic extract of Centratherum anthelminticum. The in vitro antioxidant activity was assessed using 1,1-Diphenyl-2-picrilhydrazyl (DPPH), nitric oxide, hydroxyl ion and hydrogen peroxide for free radical scavenging properties of the extracts, and determining their phenol and flavanoid contents. The antidiabetic activity was tested in rats following streptozotocin diabetes induction. The in vitro antioxidant activity using DPPH, nitric oxide radical, hydroxyl radical and H2O2 radical showed IC50 value 68.27 ± 0.34, 82.05 ± 0.46, 113.49 ± 0.86 and 94.71 ± 0.78 μg/ml respectively. The hydroethanol extract of plant seed (250, 500 and 750 mg/Kg) exhibited significant antidiabetic activities with 40.46%, 56.37% and 52.15% glycaemia reduction, respectively. These findings suggest that C. anthelminticum seeds possess both antidiabetic and antioxidant properties, and therefore could be used as starting point for the development of herbal medicines and/or source of new drug molecules...
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Posted by admin on Mar 30, 2015 in |
The objective of this study was to evaluate the possible antimicrobial properties of water, methanol, chloroform and Petroleum ether extracts of leaves of different Indian medicinal plants including Mangifera indica Linn, Ixora parviflora Vahl, Psydium guajava Linn, Calotropis procera Ait. The extracts were investigated individually for in vitro antibacterial activity by cup plate (well diffusion) method against nine pathogenic bacteria cultures. Of all the plants investigated for antimicrobial potential, the leaf extracts of Psydium guajava showed high antimicrobial activity against the tested microorganisms. Methanolic leaf extract of Psydium guajava was found to exhibit highest antimicrobial activity. Water leaf extracts of Mangifera. Indica and Ixora parviflora did not show antimicrobial activity. Phenolic content of Psydium guajava could contribute towards its antibacterial activity. The antibacterial activities of the investigated plants could make it useful in microbial...
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Posted by admin on Mar 30, 2015 in |
Nilavembu Kudineer, a Siddha Polyherbal formulation, is a decoction concentrate widely used in Siddha Medicine to combat major type(s) of fevers including viral origin such as Dengue. Methodology: Study type: Prospective case-control with Retrospective data collection. Study Setting: National Institute of Siddha – it is a central Government Siddha Hospital, located at Chennai, Tamil Nadu and considered as an apex Institute for Siddha Medicine in India. Study Subjects: Patients reported at OPD. Inclusion Criteria: Fever patients with clinical symptoms of viral fever and / or Thrombocytopenia as “Cases”. Patients without symptoms of viral fever but age-sex matched with case-subject as “Controls”. A person who has consumed minimum 5 days of Nilavembu Kudineer was considered as Nilavembu consumed person. Exclusion Criteria: Malarial fever, Enteric fever, Filarial fever, Sepsis, Leptospirosis etc. Sample size: 176 cases and 352 controls. Study period: 4 months. Statistical tools: Odds Ratio, Conditional Logistic regression. Results: The odds of Nilavembu Kudineer consumed persons for developing viral related fever was 0.24 (CI: 0.13 to 0.45) which was statistically...
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