Posted by admin on Feb 25, 2015 in |
The antioxidant activity of cultured mycelia of ten species of the oyster mushroom, Pleurotus species namely P. ostreatus, P. flabellatus, P. sapidus, P. citrinopileatus, P.roseus, P.sajor-caju, P.florida, P.cystidouses, P.fossulatus, and P.eryngii was examined. The extracts were screened for their ability to scavenge DPPH, hydroxyl, superoxide radicals and also for ferric reducing power and lipid peroxidation inhibiting activity. Among the ten species studied, all were found to possess DPPH radical scavenging and lipid peroxidation inhibiting properties. P florida scavenged DPPH radicals (94.02%) and P.citrinopileatus inhibited the lipid peroxidation (76.65%) more efficiently than others. Five among the ten Pleurotus species showed ferric reducing power, and SOD activity. P.ostreatus efficiently reduced ferric radicals (51.06%) and scavenged super oxide radicals (74.61%) when compared to others. From the ten species studied all except P.roseus scavenged hydroxyl radicals and the maximum activity was showed by P. sajor-caju (76.17%). The present study thus reveals the profound free radical scavenging activity of cultured mycelia of oyster mushrooms. The findings suggest the potential use of cultured mycelia of...
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Posted by admin on Feb 25, 2015 in |
Oral route of drug administration have wide acceptance up to 50-60% of total dosage forms. Solid dosage forms are popular because of ease of administration, accurate dosage, self-medication, pain avoidance and most importantly the patient compliance. This study was aimed at development of Propranolol hydrochloride and flunarizine dihydrochloride conventional dissolving tablets in combined dosage form. Propranolol competes with sympathomimetic neurotransmitters such as catecholamines for binding at beta (1)- adrenergic receptors in the heart, inhibiting sympathetic stimulation and Flunarizine is a selective calcium entry blocker with calmodulin binding properties and histamine H1 blocking activity both are effective in the treatment of coexistence of hypertension &...
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Posted by admin on Feb 25, 2015 in |
A moderately halophilic strain of actinomycete HB-11, having ability to produce antifungal compound was isolated from soil samples collected from the region of Mumbai sea port, India. The strain HB-11 was identified as Streptomyces werraensis on the basis of its morphological characteristics and 16S rRNA gene sequencing. It is found to be active against both filamentous as well as yeast fungi including Aspergillus parasiticus, Aspergillus niger, Aspergillus flavus, Ustilago maydis, Helminthosporium gramineum, Alternaria solani, Mucor liomelis, Rhizopus oryzae, Sclerotium rolfsii, Fusarium moniliforme, Fusarium oxysporum and Candida albicans. The cultural conditions for the production of antifungal metabolite were optimized by using various parameters and using U. maydis as indicator test strain. The antifungal metabolite was extracted with organic solvents, purified by column chromatography and its polyene nature was determined by Ergosterol plate assay. The optimization studies for antifungal compound production revealed that the suitable period of incubation as 8 days, pH as 8.0, salinity (5%), starch and ammonium sulphate as suitable carbon and nitrogen sources respectively and presence of magnesium...
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Posted by admin on Feb 25, 2015 in |
The aim of this study is to investigate the cholesterol lowering property of bacterial isolates. Different cultures of the bacteria were isolated from cow milk. Out of the cultured bacterial isolates Lactobacillus species, Micrococcus luteus and Staphylococcus aureus were screened. Three isolates such as Lactobacillus plantarum, Lactobacillus fermentum, Lactobacillus casei were identified based on morphological, physiological and biochemical characteristics. Sugar fermentation test was performed to identify Lactobacillus species. The sugars such as D- glucose, galactose, lactose, mannitol and D-fructose were used. The five isolates were inoculated on Nutrient agar medium with cholesterol (1mg/1ml) concentration and incubated for 24 hours to check the cholesterol utilization. Cholesterol lowering activities of these organisms were checked at different concentration of cholesterol, different pH, different temperature and different incubation time. The results of this study, suggests that Lactobacillus plantarum was an effective organism for cholesterol utilization at pH 7, temperature at 35ºC and incubation time was at 24 hrs. Hence Lactobacillus plantarum has been reported as cholesterol lowering...
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Posted by admin on Feb 25, 2015 in |
In the traditional system of Indian medicine Caesalpinia bonducella Flem. is widely used for its medicinal properties. All parts are valuable as it contains various secondary metabolites. Seeds contain 20-30% of oil. On the basis of phyto-chemical analysis of the seed it is known to contain alkaloids, flavonoids, glycosides, saponins and furano di terpenes. The plant possesses antidiabetic, antioxidant, antitumor, antiproliferative and anti imflammatory activities. “Cytotoxicity” is the ability to kill the cells. In the present study experiments were carried out to check for the cytotoxic nature of seeds and callus using Daphnia cultures. The method used is simple, inexpensive and even a low toxin amounts are sufficient to perform the test. Cytotoxic nature was tested using aqueous extracts of seed powder as well as callus. From the experiments it was observed that extract from seed powder as well as callus expressed cytotoxic activities, however, seed powder extract was more effective compared to callus...
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