Posted by admin on Jan 30, 2015 in |
Lannea kerstingii Engl. & K. Krause stem bark (Anacardiaceae) is used to treat anaemia, malaria, infections and Burili ulcer. The antioxidant, antihelmintic and trypanocidal properties have been demonstrated using appropriate in vitro and in vivo assays. But the effective doses have demonstrated toxic effects such as a significant decrease of body weight of the experimental animals. This work aimed to evaluate the 60 days repeated-dose toxicity of hydroalcoholic extract from Lannea kerstingii stem bark. After 60 days administration, L. kerstingii induced 50% of rats death; accumulation of gas in the digestive tract and a significant decrease of rats body weight. The determination of the toxic mechanism has shown that L. kerstingii decreased the daily food intake and the intestinal motility. The decrease of the intestinal motility by I.P. administration was higher than by P.O. administration. Naloxone, inhibited the effect of L. kerstingii on the intestinal motility. L. kerstingii stem bark hydroalcoholic extract possess a significant inhibition effect on ileum contraction. Therefore it can be toxic in prolonged use at...
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Posted by admin on Jan 30, 2015 in |
The association between chronic bronchitis and cataract is debating. While some referred it to the corticosteroid therapy commonly prescribed to bronchitis, other named different immunological pathways that may be involved in this relationship. We tried to find out the possible immunological benefit of phosphodiesterase IV inhibition in these two common associated conditions. The rats were divided into 4 groups; I: control , II: Selenium (Se), III: roliprampretreated and IV: rolipram post treated groups. Lenses and lung tissues were collected by the end of 2nd week. Upper lobe of right lungs was used to calculate the wet/dry ratios. Histological examinations using H&E, Malloryʼs trichrome, Proliferation cell nuclear antigen (PCNA) and nuclear factor (NF) Kappa B immunohistochemistry study of the lung were done. Scanning electron microscope and H&E studies of lenses were performed. The lung showed significant (P<0.05) elevation of wet/dry ratios with Se administration. There was infiltration by inflammatory cells, congested blood vessels with thickened wall, collagen fibers were increased (P<0.05) with intense NF-Kappa B immuno-reaction. Moreover, there was increase...
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Posted by admin on Jan 30, 2015 in |
The glucosamine-6-phosphate isomerase and Jacob glycoprotein gene expression levels were assessed during E. histolytica excystation from the feces of five patients. Excystation was performed at 0, 24, 48 and 96 h in Robinson medium, and RNA was extracted from the cysts and trophozoites. cDNA was synthesized to amplify the genes using real-time RT-PCR. The results demonstrated differences according to the incubation time and/or conversion of the cystic phase to the trophozoite, where the most important expression in the genes was observed at 96 h excystation. The expression levels of the genes studied positively correlated with the population of trophozoites during...
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Posted by admin on Jan 1, 2015 in |
Two flavonoids were isolated by performing column chromatographic separation of methanol extract of ariel parts of Derris brevipes (Benth) Baker. Structural elucidation of purified compounds was done on the basis of spectral analysis using IR, 1H NMR, 13C NMR 2D NMR spectroscopy and Mass spectrometry. The structures of the flavonoids were elucidated as 2’, 4’-dihydroxy-4-methoxy-3’-prenylchalcone (compound1) and Leutolin-3′-O-xilofuranose (compound...
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Posted by admin on Dec 31, 2014 in |
Hypobaric hypoxia induced systolic heart dysfunction and arrhythmia is prevalent in ascendants. L-type calcium channel blocker amlodipine plays important role in modulating cardiac function. We tested if amlodipine could impart cardio protection and restore oxidative balance under hypobaric hypoxia, by modulating HIF-1α activity and calcium accumulation, thus, protects from cardio-toxicity. Experimental animals, male Sprague-Dawley rats (180-200 g), were randomized into four groups, i.e. normoxia control (N), normoxia + amlodipine (N+AML), hypoxia control (H) and hypoxia + amlodipine (H+AML) (n=8), and exposed to hypobaric hypoxia for three time points i.e. 24 h, 48 h and 72 h to assess cellular damages. Amlodipine treatment not only restored systolic blood pressure and heart rate to normal levels under hypobaric hypoxia, but also regulated HIF-1α expression, Akt/p-Akt levels and intracellular Ca2+ levels ([Ca2+]i).Biochemical markers of oxidative damage, i.e. glutathione reduced (GSH), glutathione oxidized (GSSG), lipid peroxidation (MDA) and free radical generation by reactive oxygen species (ROS) estimation were significantly restored by intraperitonial administration (10 mg / kg b.w.) of amlodipine. Amlodipine was found...
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