Posted by admin on Feb 25, 2015 in |
Plants in Combretaceae family are traditionally reputed to be anthelmintics and antimicrobial agents. Dried aerial parts of Combretum sordidum (Exell) were ground to fine powder and extracted using ethanol. Ethanol extract was partitioned to obtain hexane and ethylacetate extracts successively. These extracts were screened for secondary metabolites using standard methods and evaluated for their antimicrobial potentials against two Gram positive bacteria; Bacillus subtilis (ATCC 14579) and Bacillus cereus (ATCC 33923); two Gram negative bacteria; Proteus mirabilis (ATCC 21784), Salmonella typhi (ATCC 25179) and a fungus; Candida albicans (NCTC 227) by tube dilution method. Essential oil from its leaves was obtained by hydrodistillation method and analyzed by GC and GC-MS. Preliminary phytochemical screening revealed the presence of alkaloids, saponins, tannins, flavonoids, cardiac glycosides, steroids and anthraquinones in the ethylacetate and ethanol extracts. Both extracts displayed broad spectrum activities against the organisms used with minium inhibitory concentration (MIC) at 400µg/mL. Five compounds comprising 99.99% of the total peak area were identified from the volatile oil. The most abundant constituent was 7-...
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Posted by admin on Feb 25, 2015 in |
The investigation of terrestrial plants origin aimed at searching new natural biologically active compounds is a central issue of this kind of studies, through structure elucidation combined with biological tests. We investigated the biologically active secondary metabolites from Leucas lavandulaefolia towards various effects on the behavior of Aphanomyces cochlioides zoospores and with antimicrobial activity. We purified the following compounds acacetin, chrysoeriol, luteolin, acacetin 7-O-β-D-glucuronide, acetoside, isoacetoside, salicylic acid and caffeic acid. Their structures were elucidated by spectroscopic analyses and compared with those of the reported data. Five compounds (luteolin, acacetin 7-O-β-D-glucuronide, acetoside, isoacetoside, and salicylic acid) were isolated from L. lavandulaefolia for the first time ever. It was found that luteolin showed a strong attractant and encystment activity toward zoospores of the phytopathogenic fungus A. cochlioides zoospores. Chrysoeriol also showed an attractant activity towards A. cochlioides zoospores. This is the first research report of L. lavandulaefolia regarding zoospores bioassay. Antibacterial assay on these compounds indicated that they have antimicrobial activity against the tested bacteria Bacillus subtilis and Staphylococcus aureus....
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Posted by admin on Feb 25, 2015 in |
The Antimicrobial (antifungal and antibacterial) properties of the different solvents extracts (benzene, pet ether, acetone, chloroform, methanol and aqueous) of Rumex vesicarius Linn plant plants, on four bacterial (Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus) and four fungal isolates (Aspergillus niger, Tricoderma reesei, Penicillium funiculosum and Fusarium oxysporum) were investigated by well diffusion assay. The minimum inhibitory concentration (MIC) of these solvents extracts evaluated at different concentration (3.25 mg/ml, 6.25 mg/ml, 12.5 mg/ml, 25 mg/ml and 50 mg/ml) for the tested organisms. The widest spectrum of antimicrobial activity was recorded for pet ether extract of leaf against P. funiculosum (30±1.2mm), F. oxysporum (22±0.8mm), E. coli(20±0.9mm) and P. Aeruginosa (10±0.7mm). This was followed by the benzene extract of leaf, which inhibits P. funiculosume t4l(20±0.8mm), F. oxysporum (18±0.4mm), E.coli(10±0.7mm). Acetone extract of flower work against P. funiculosum (30±1.4mm), A. niger, (12±0.7mm), E. coli(20±0.6mm) and S.aureus (18±0.8mm). The inhibitory effect of chloroform extract of flower against A.niger, (18±0.3mm) and F. oxysporum (16±0.3mm). The antimicrobial activity of methanolic and aqueous extract...
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Posted by admin on Feb 25, 2015 in |
Habenaria plantaginea Lindl. (Orchidaceae) is used as a medicinal plant in several countries. In this study, ethanol, chloroform and water extracts of Habenaria plantaginea were evaluated for antibacterial activity against bacterial (MTCC) strains. Antibacterial activity of this plants extracts were performed using agar disc diffusion method. The antibacterial activity of ethanol extracts showed more effective followed by water and chloroform extracts against all the bacterial strains. Preliminary phytochemical studies were performed for the presence and absence of secondary metabolites in the whole plant. The Minimum Inhibitory concentration (MIC) exhibited by H. plantaginea extract against the bacterial strains ranged between 0.125 mg/ml to 6 mg/ml. The presence of bioactive constituents is associated with the antimicrobial activity of various solvent extracts of this plant was carried in attempt to support the use by medicinal practitioner for the treatment of various diseases. Medicinal importance of this orchid will target to meet the therapeutic...
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Posted by admin on Feb 25, 2015 in |
A new RP-HPLC method was developed and validated for the simultaneous determination of amprolium hydrochloride and sulfaquinoxaline sodium in a powder dosage form. The chromatographic separation was achieved by isocratic mode with Phosphate buffer and Acetonitrile (30:70; v/v), as mobile phase and using C-18 RP-Amide (25.0cm × 4.6 mmi.d, 5µ particle size) as stationary phase, with a flow rate of 1.0 ml/min at ambient temperature. The quantification of the analytes was monitored by UV detection at a wavelength of 263 nm. The optimized method was subjected to validation for specificity, accuracy, precision, linearity, range, ruggedness and robustness. The calibration graphs were linear in the concentration range of 0.08 – 0.12 mg/ml. The results were evaluated as per the International Conference on Harmonization (ICH) guidelines. The precision of the method in terms of RSD is not more than 2% for both the analytes. The method is sensitive, specific, fast and accurate with minimum sample...
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