Posted by admin on Dec 1, 2014 in |
Various parts of Lasia spinosa (Linn.) are widely used in North East region to manage wide range of disease but so for no scientific study was done to find out its pharmacological properties which may support its use in traditional medicine. Bioactive fraction (ethyl acetate fraction, LSE) from the methanol – water (80:20) extract of Lasia spinosa rhizome was evaluated for gastroprotective and antioxidant activity. In the safety evaluation, LD50 was found to be more than 2000 mg/kg. High performance thin layer chromatography (HPTLC) finger printing of the total extract was also carried out using gallic acid as markers in an attempt to characterize the constituents responsible for the activities and also to standardize the extract. The bioactive fraction from Lasia spinosa rhizome (LSE; 50 and 100 mg/kg body weight) was administered orally, twice daily for 5 days for prevention from Ethanol (EtOH)-, cold-restraint stress (CRS) – and pylorus ligation (PL)-induced ulcers. Estimation of antioxidant enzymes activity was carried out in CRS-induced ulcer model. LSE showed dose-dependent ulcer protective...
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Posted by admin on Dec 1, 2014 in |
This study focused on an investigation of dissolution property of solid dispersion system consisting of drug, exipients, and carrier. The purpose of this study was to enhance the dissolution of Atorvastatin Calcium, a practically water-insoluble drug by preparation of solid dispersion using hydrophilic polymers and a carrier PEG 6000. Solid dispersion formulations were prepared by using The Melt Solvent and physical mixing method. Physical mixtures (PMs) of atorvastatin and hydrophilic polymer Kollicoat IR and PVK 30 were prepared at 1:0.5, 1:1 and 1:2 ratios. Melt Solvent method was used to prepare solid dispersion of atorvastatin, PVK 30 and kollicoat IR at 1:2 ratio. Solid dispersions were characterized by differential scanning calorimetry, scanning electron microscopy and dissolution tests. Characterization studies revealed that solid dispersion of Atorvastatin prepared by Melt Solvent methods showed better dissolution compare to Physical mixing and pure atorvastatin due to the conversion of atorvastatin into a less crystalline and/or amorphous form. The order of dissolution enhancement was Kollicoat IR > PVK30 in solid dispersions as well as...
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Posted by admin on Dec 1, 2014 in |
Anti-obesity effect of Cinnamaldehyde and Orlistat on High fat diet induced obese rats was investigated. 30 male wistar albino rats were divided into 5 groups: control group fed on normal standard diet; HFD group; Orlistat (HFD+ 50mg/kg body weight; CA (HFD+ 40mg/Kg body weight) and CA (HFD+ 80mg/Kg body weight) for 8 weeks. The anti obesity activity of CA and OR was estimated in terms of Food intake, body weight gain, feed efficiency ratio, lipid profile, AI, CRI and faecal lipids. Obesity induced group of rats showed significant (p< 0.001) increase in body weight, feed consumption, TG, TC, LDL-c, VLDL-c in serum, AI, CRI and decrease with HDL-c level (p< 0.001) compared with the normal group of rats. The BWG and FER were reduced in CA treated rats. CA treatment also resulted in significant (p< 0.001) decreases in serum TC, TG, LDL-c, AI and CRI and increased (p< 0.001) HDL-c concentrations in a dose dependent manner compared with untreated obese rats. The results were comparable with Orlistat, a standard...
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Posted by admin on Dec 1, 2014 in |
An accurate, precise, specific and rapid RP-HPLC method was developed and subsequently validated for the determination of Dapagliflogin in API. Better separation of the drug was achieved on BDS column (250×4.5mm, 5µ) with a mobile phase consisting of a mixture of ortho phosphoric acid and acetonitrile (45:55 v/v) at a flow rate of 1ml/min, with detection at 245nm using Photo Diode Array (PDA) detector. The developed method was validated for different parameters such as linearity, accuracy, precision, limit of detection(LOD), limit of Quantitation (LOQ), robustness and the results were found to be within the limits according to ICH (International Conference on Harmonization) guidelines. The retention time was found to be 2.963 min. The method was found to be linear in the range of 25-150µg/ml with a correlation coefficient (r2) of 0.999. The LOD and LOQ of the method were calculated to be 0.6 and 1.8µg/ml respectively. The Precision was estimated by employing repeatability; intra-day and inter-day studies and the results were calculated as %RSD values and were found to...
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Posted by admin on Dec 1, 2014 in |
Objective: Potato (Solanum tuberosum L.) is a major world food crop next in production to maize, rice and wheat. Potatoes are a source of dietary energy due to their carbohydrate level and also contain a high value of vitamins. The objective of this study was determine the phytochemical constituents and antimicrobial activities of four common potato varieties developed by CPRI (Central Potato Research Institute, Shimla, India) i.e. Kufri Bahar, Kufri Badshah, Kufri Pukhraj and Kufri Puskar. Methods: The antimicrobial activity of selected plant spices against the mentioned microorganisms and their effectiveness were assessed by the presence or absence of inhibition zones and zone diameter by using Agar well diffusion technique and some chemical tests were performed for analysis of phytochemical constituents. Results: The antimicrobial activity of all the potato extracts showed the significant inhibitory activity against all the bacteria and fungi strain. Conclusion: The demonstration of broad spectrum activity of potato varieties may help to discover new chemical classes of antibiotic substances that could serve as selective agents for...
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