Posted by admin on Dec 1, 2014 in |
A simple, sensitive, linear, precise and accurate RP-HPLC method for simultaneous estimation of Zaltoprofen and Paracetamol in bulk and tablet formulation was developed and validated. The proposed RP-HPLC method utilizes Phenomenex C18 column (150 ×4.6 mm i.d., 5 µm), optimum mobile phase consisted of isocratic run of Phosphate buffer (pH-4.2): acetonitrile (40:60V/V) with the effluent flow rate of 1.0ml/min, and UV detection wavelength 260 nm. The developed method was statistically validated for linearity, precision, robustness, ruggedness and specificity. The method was linear over the range of 2-10 µg/ml for Zaltoprofen and 8-40 µg/ml for Paracetamol. The mean recovery was 99.27% and 99.76% for Zaltoprofen and Paracetamol respectively. The intermediate precision data obtained under different experimental setup was quite concurrent with less critical %RSD. The proposed method can be useful in the quality control of Zaltoprofen and Paracetamol in their combined dosage...
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Posted by admin on Dec 1, 2014 in |
Aims: Detection and isolation of restriction endonucleases like topoisomerase Thermophilic Bacillus subtilus strain RC1. Methods and results: Topoisomerase like enzyme activity is detected from thermophilc Bacilus sp. The isolated enzyme is partially characterized. The molecular weight of the enzyme is ~ 33 KDa and the optimum temperature for the activity is 37 0C. The crude and gel purified enzyme activity were performed. The enzyme could be novel one, as the isolate is from hot water ecosystem. The restriction pattern of the new isolated enzyme is dissimilar to the commercially available enzymes. The enzyme retained the activity at a storage temperature of 4 0C about one to two months. Magnesium chloride is found to be essential for the activity. The enzyme activity pattern is like topoisomrase. Activity is enhance in presence of ATP. The further characterization of topo like enzyme is in contined.Conclusion: The endonuclease type of topoisomerase enzyme is detected and isolated from thermophilic Bacillus sp.The restriction pattern studies indicate that the enzyme cuts standard plasmid DNA. Significant impact...
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Posted by admin on Dec 1, 2014 in |
The present chemical analysis deals with Asparagus racemosus root for phytochemical screening and GC-MS analysis. Phytochemical constituent of the root extract was carried out according to the standard method. GC-MS analysis was performed on the methanolic extract of A. racemosus to find out the chemical constituents. The phytochemical screening revealed the presence of flavonoids, alkaloids, steroids, terpenoids, glycosides, tannins, terpenoids, saponins. GC-MS results revealed the presence of 6 different phytochemical constituents like, 2-Propanone, 1,3-dihydroxy 1), 2-Fruancarboxy aldehyde, 5-(hydroxymethyl) 2), Hexadecanoic acid 3), n-Hexadecanoic acid 4), Ethanol,2(Octyloxy)- 5), 1,9-Nonanediol 6). The presence of various biological active compounds confirms the application of A. racemosus for various diseases by conventional practitioners. The obtained phytochemical constituent results demonstrate that the methanol root extract of Asparagus racemosus could be used corn foot, anticancer, diabetic healer. However, isolation of individual phytochemical constituents might be useful to formulate a novel...
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Posted by admin on Dec 1, 2014 in |
Andrograhis paniculata is a wonder drug using in traditional systems of medicine for multiple clinical applications. This research article deals with the phytochemical and antioxidant properties of A. paniculata leaves. Qualitative and quantitative phytochemical analyses were carried out by TLC and HPLC method. Physico-chemical analysis was done to standardize the plant drug material so as to ensure the use of genuine material. The result of phytochemical studies confirmed the presence of various secondary metabolites like alkaloids, glycosides, flavonoids, proteins, tannins, phenols, saponins, terpenoids, quinones and steroids. TLC studies of chloroform extracts showed 3 spots and methanol extracts showed 5 spots at different Rf values. HPLC analysis confirmed the presence of the major active constituent andrographolide. Effect of age on quantity of andrographolide content was studied and it was found to be higher in 120 day old plants. Antioxidant property was determined by reducing power method. Maximum antioxidant activity was observed in pure andrographolide followed by methanol and water extracts of leaf...
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Posted by admin on Dec 1, 2014 in |
This study was conducted as a single blind, randomized and standard controlled clinical trial in cases of Falij Nisfi (Hemiplegia) due to ischaemic stroke. Forty eligible patients of Hemiplegia were randomized into test and control groups, comprising 20 patients in each. Test group was given Nuskha munzije balghamfor12 days, followed byNuskha Mushile Balgham, mixed with Munzije Balgham for next two consecutive days in decoction form, orally. Massage was started from 15th day with Roghan Malkangani on spinal column and paralyzed limbs for 15 minutes daily till 28th day. Control group was given tablet Piracetam Hydrochloride, 800 mg twice a day. Hypertensive patients in both groups, A and B, also received tablet Aspirin, 75 mg and tablet lisinopril, 2.5 mg, once daily for 28 days. The assessment of efficacy was based on “Stroke Rehabilitation Assessment of Movement (STREAM)”. Pre and post treatment values of STREAM were subjected to statistical analysis within and between the groups using Paired‘t’ test, Wilcoxon matched pairs signed rank test, and Kruskal-Wallis test with Dunn’s multiple...
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